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The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. The built-in cooling system (Bioruptor® Cooler) ensures high precision temperature control resulting in higher quality samples.
Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.
My group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
High throughput sequencing has dramatically reduced sequencing costs and the development of duel indexing strategies makes it possible to combine 384 samples in a single sequencing run. By developing library preparation methods to enrich for retroviral integration sites and retroviral transcripts we have been able to produce large numbers of low cost libraries to track the evolution of infection and to characterise patterns of expression from our virus of interest. The Bioruptor® Pico has been essential part of our protocols and provides straightforward and consistent shearing of our samples.Dr Keith Durkin, Unit of Animal Genomics, GIGA-R, Université de Liège , 4000 Liège, Belgium
Follow our guidelines and find the good parameters for your expected DNA size: DNA shearing with the Bioruptor® Pico
The Bioruptor® NGS* is a robust user-friendly sonication system that is key for both reproducible chromatin shearing and DNA shearing. The Bioruptor® NGS consistently delivered optimal DNA shearing that was critical in generating homogeneous sequencing libraries for our experiments. We also observed excellent chromatin shearing result that were crucial for our epigenetic mapping projects.Dr. Sascha Tierling, Department of Genetics and Epigenetics, Prof . Dr. Jörn Walter's lab, Universität des Saarlandes, Germany.
The new Bioruptor® Pico machine has reduced the amount of time spent sonicating Chromatin by a massive amount. Some protocols require quite harsh fixing conditions which meant fragmenting DNA on the old machine was taking many rounds and several times. With the new Bioruptor® Pico machine these sonications were taking just one round of 10 cycles thereby reducing the fragmentation time substantially. Following sonication, I have used the new IDeal ChIP-seq kit. This is a nice straight forward kit that if followed with an appropriate chip validated antibody gave amazing chip-seq results that worked time and again with several different transcription factors. I would recommend both kits for good, consistant chromatin work.Dr. Karen Dawson, RNA Biology Group, Cancer Research UK Manchester Institute at the University of Manchester
Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford
We are particularly satisfied with the Bioruptor® Pico that we have recently purchased. Since we have started using this novel machine, all experiments for our epignetics research have been much more easy to perform. This machine is really a great thing for us. The whole department has now started using this novel device.Dr. Marco Pontoglio, Head of the Team "Expression Génique, Développement et Maladies" (EGDM) and Director of the “Development, Cancer and Reproduction”, Department Institut Cochin, Université Paris-Descartes
I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany
I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.
Thanks Diagenode for saving my PhD!Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin
Neonatal screening cards provide a valuable bio-bank for retrospective studies. We have used the Bioruptor Pico and IP-Star Compact instrument along with the Auto MeDIP and IPure v2 kit to generate genome-wide DNA methylation profiles using only minute DNA inputs. Using the Diagenode instruments and products has generated reproducible results and saved us hands-on time. We are satisfied with the level of service and technical support and would recommend Diagenode to all others working in the field of epigenetics.
|Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico|
Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
|Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico|
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...
|Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl|
HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleu...
|Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml|
Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin Shearing...
|Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder|
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
|Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor® |
Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene...
|DNA Shearing for Bioruptor® Pico - Standard 100 μl Volume|
Standard protocols for programmable DNA size distributions, excellent reproducibility, and high d...
|DNA Shearing for Bioruptor® Pico - 50 μl Volume |
For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptor...
|DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder|
Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
|DNA Shearing with Bioruptor® Pico - Small Volume (10 µl) |
For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. ...
|FFPE DNA extraction protocol using the Bioruptor®|
The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
|iST Sample Preparation with the Bioruptor®|
The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...
|PCR amplicons shearing using the Bioruptor Pico|
With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR ...
|Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico|
Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
|Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico|
ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome...
|RNA Shearing for Bioruptor® NGS and Pico|
The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, how...
|Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation|
Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology....
In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critic... Read more
The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
|FFPE DNA extraction|
Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraf... Read more
|Next Generation Sequencing|
DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA libr... Read more
Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such... Read more
|Methylbinding domain protein|
The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containin... Read more
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichme... Read more
|Bioruptor® Sonicator Brochure BROCHURE|
Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
|Bioruptor® DNA shearing for Agilent SureSelect library preparation: a perfect combination APPLICATION NOTE|
The Cologne Center for Genomics (CCG), headed by Prof. Dr. Peter Nürnberg, shows that t...
|Which tubes for which Bioruptor®? QUICK GUIDE|
Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
|Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing CASE STUDY|
Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center,...
|True MicroChIP and MicroPlex kits APPLICATION NOTE|
From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the ...
|ChIP-seq from human tumor tissue APPLICATION NOTE|
Application of the Bioruptor® Pico for shearing chromatin fragments for ChIP-seq from human t...
|ChIP kit results with True MicroChIP kit POSTER|
Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the g...
|Bioruptor® NGS System – Designed Exclusively for Fast & Accurate DNA Shearing POSTER|
The Bioruptor®NGS is the DNA shearing device of choice for sequencing applications, providing...
|Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS APPLICATION NOTE|
DNA shearing is important step for next-generation sequencing library preparation. Important is r...
|DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder PROTOCOL|
Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
|Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder PROTOCOL|
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
|Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing APPLICATION NOTE|
Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o...
|Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit APPLICATION NOTE|
Next Generation Sequencing workflows include a number of critical steps which can have a signific...
|Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico APPLICATION NOTE|
The chromatin shearing is very important step for all kind of ChIP analysis followed by next-gene...
|Ultrasensitive proteomics for microdissected fresh frozen tissue samples APPLICATION NOTE|
This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quant...
|Bioruptor® Pico Sonication System Manual MANUAL|
The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as...
Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage
Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress
Analysis of Meiotic Double-Strand Break Initiation in Mammals
Differential α-synuclein expression contributes to selective vulnerability of hippocampal neuron subpopulations to fibril-induced toxicity
CRL7SMU1 E3 ligase complex driven H2B ubiquitination functions in sister chromatid cohesion by regulating SMC1 expression
Lyar-mediated recruitment of Brd2 to the chromatin attenuates Nanog downregulation following induction of differentiation
The transcription factors Runx3 and ThPOK cross-regulate acquisition of cytotoxic function by human Th1 lymphocytes
β1-integrin impacts Rad51 stability and DNA double-strand break repair by homologous recombination
TGFβ signaling hyperactivation-induced tumorigenicity during the derivation of neural progenitors from mouse ESCs
MAX to MYCN intracellular ratio drives the aggressive phenotype and clinical outcome of high risk neuroblastoma
SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-κB pathway
A novel long noncoding RNA LINC00844 regulates prostate cancer cell migration and invasion through androgen receptor signaling
APRDX1 mutant allele causes a MMACHC secondary epimutation in cblC patients
TBR2 antagonizes retinoic acid dependent neuronal differentiation by repressing Zfp423 during corticogenesis
The CUE1 domain of the SNF2-like chromatin remodeler SMARCAD1 mediates its association with KRAB-associated protein 1 (KAP1) and KAP1 target genes
Repeated methamphetamine and modafinil induce differential cognitive effects and specific histone acetylation and DNA methylation profiles in the mouse medial prefrontal cortex
Super enhancer associated RAI14 is a new potential biomarker in lung adenocarcinoma
Transcription Factor YY1 Modulates Lung Cancer Progression by Activating lncRNA-PVT1
Epigenetic control of epilepsy target genes contributes to a cellular memory of epileptogenesis in cultured rat hippocampal neurons
The cardiac calsequestrin gene transcription is modulated at the promoter by NFAT and MEF-2 transcription factors
The CCAAT box in the proximal SERCA2 gene promoter regulates basal and stress-induced transcription in cardiomyocytes
Coordinated Transcriptional Control of Adipocyte Triglyceride Lipase (Atgl) by transcription factors Sp1 and PPARγ during Adipocyte Differentiation
Novel Molecular Mechanism of Regulation of CD40 Ligand by the Transcription Factor GLI2
Egr2 and 3 Inhibit T-bet–Mediated IFN-γ Production in T Cells
Foxa2, a novel protein partner of tumour suppressor menin, is deregulated in mouse and human MEN1 glucagonomas
m6A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition
A set of novel automated quantitative microproteomics protocols for small sample amounts and its application to kidney tissue substructures
Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase
DNA fragmentation and quality control analysis
Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
UMI-4C for quantitative and targeted chromosomal contact profiling
Characterization of novel Bovine Leukemia Virus (BLV) antisense transcripts by deep sequencing reveals constitutive expression in tumors and transcriptional interaction with viral microRNAs
Parental epigenetic asymmetry of PRC2-mediated histone modifications in the Arabidopsis endosperm
Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock
Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.
TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint
The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis
Desensitization and incomplete recovery of hepatic target genes after chronic thyroid hormone treatment and withdrawal in male adult mice
Transducin-like enhancer of split-1 is expressed and functional in human macrophages
Standardizing chromatin research: a simple and universal method for ChIP-seq
The Deacetylase Sirtuin 1 Regulates Human Papillomavirus Replication by Modulating Histone Acetylation and Recruitment of DNA Damage Factors NBS1 and Rad51 to Viral Genomes
The MEF2-HDAC axis controls proliferation of mammary epithelial cells and acini formation in vitro
LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2
Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration
Concurrent whole-genome haplotyping and copy-number profiling of single cells.
High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq.
Mitogenomic phylogeny of the common long-tailed macaque (Macaca fascicularis fascicularis).
Phylogenomics of Phrynosomatid Lizards: Conflicting Signals from Sequence Capture versus Restriction Site Associated DNA Sequencing
Modulation of p53 and met expression by Krüppel-like factor 8 regulates zebrafish cerebellar development.
Chromatin DNA RNA
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