Diagenode

Bioruptor® Pico sonication device





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DNA methylation of the Tacr2 gene in a CUMS model of depr...

BRAIN RESEARCH

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Best Workflow Practices for
ChIP-seq Analysis
Temperature-controlled
Easy to use
All-in-one solution
Processing of 6-16 samples
Sample size 5 µl – 2 ml
No soundproof box
  •  Protocols
  •  Documents
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Catalog Number
Format
B01060010
1 unit

The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. The built-in cooling system (Bioruptor® Cooler) ensures high precision temperature control resulting in higher quality samples. 

  • All-in-one shearing solution : fully-validated tubes and kits available
  • Simultaneous sonication of 6-16 samples
  • Small, light, and easy to use
  • Temperature-controlled

Ideal for

  • DNA shearing for Next-Generation-Sequencing (5 - 100 µl)
  • Chromatin shearing (10 µl - 2 ml)
  • RNA shearing
  • Protein extraction from tissues and cells
  • FFPE DNA extraction
  • Protein aggregation studies

Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.

TESTIMONIAL

We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.

Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany
TESTIMONIAL

High throughput sequencing has dramatically reduced sequencing costs and the development of duel indexing strategies makes it possible to combine 384 samples in a single sequencing run. By developing library preparation methods to enrich for retroviral integration sites and retroviral transcripts we have been able to produce large numbers of low cost libraries to track the evolution of infection and to characterise patterns of expression from our virus of interest. The Bioruptor® Pico has been essential part of our protocols and provides straightforward and consistent shearing of our samples.

Dr Keith Durkin, Unit of Animal Genomics, GIGA-R, Université de Liège , 4000 Liège, Belgium
TESTIMONIAL

My group is mostly focused on epigenetic reprogramming and I have been using Diagenode products for the last 5 years. My experience with both antibodies, ChIP-Kits and the Bioruptor is nothing but positive. Diagenode products are unique for reproducibility, and this has always been a great plus for the success of my experiments.

Dr. Raffaele Teperino - Environmental Epigenetic Group - Institute of Experimental Genetics, Helmholtz Zentrum Muenchen GmbH, Germany
  • User manual
  • Recommended settings for DNA shearing with Bioruptor® Pico

    Follow our guidelines and find the good parameters for your expected DNA size: DNA shearing with the Bioruptor® Pico

  •  Testimonials

    Neonatal screening cards provide a valuable bio-bank for retrospective studies. We have used the Bioruptor Pico and IP-Star Compact instrument along with the Auto MeDIP and IPure v2 kit to generate genome-wide DNA methylation profiles using only minute DNA inputs. Using the Diagenode instruments and products has generated reproducible results and saved us hands-on time. We are satisfied with the level of service and technical support and would recommend Diagenode to all others working in the field of epigenetics.

    Read the full abstract for Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots

    Dr. Nicklas Heine Staunstrup, Faculty of Health, Institute of Biomedicine, Aarhus University, Denmark

    I work with Diagenode’s Plant ChIP-seq kit and shear the DNA on the Bioruptor Pico for the last year and I have to say that these two products saved my PhD project! Some time ago, our well-established ChIP protocol suddenly stopped to work and after long time of figuring out the reason, we invested into Bioruptor Pico. I am very satisfied from the way it works, plus it’s super quiet! Combining the sonicator with the Plant ChIP-seq kit we finally got things working. I have also decided to try the Microplex Library Prep kit, which is amazing. I have been working with other kits and I find this one efficient and very easy to use. Recently, I have tested one of the epigenetics antibody (H3K4me3) and it works very well on the plant tissue, together with the ChIP-seq kit and Bioruptor.

    Thanks Diagenode for saving my PhD!

    Kamila Kwasniewska, Plant Developmental Genetics, Smurfit Institute, Trinity College, Dublin

    I have extensively used the antibodies against the histone modifications H3K4me3, H3k27me3, H3K9ac, H4k8ac and H3K18ac provided by Diagenode. The high level of specificity and selectivity of these antibodies in mouse brain samples, confirmed by using several negative and positive controls run in parallel with mouse brain tissue samples, ensured successful and reproducible results. I have been a Diagenode costumer for over one year now and I am extremely satisfied with the efficiency of the Bioruptor Pico for chromatin shearing as well as all of the ChIP materials (i.e., antibodies, blocking peptides, primer pairs for qPCR) provided by this company. Many thanks.

    Dr. Ermelinda Lomazzo, Institute of Physiological Chemistry, AG Prof. Beat Lutz. University Medical Center of the Johannes Gutenberg University Mainz, Germany

    We are particularly satisfied with the Bioruptor® Pico that we have recently purchased. Since we have started using this novel machine, all experiments for our epignetics research have been much more easy to perform. This machine is really a great thing for us. The whole department has now started using this novel device.

    Dr. Marco Pontoglio, Head of the Team "Expression Génique, Développement et Maladies" (EGDM) and Director of the “Development, Cancer and Reproduction”, Department Institut Cochin, Université Paris-Descartes

    Diagenode has provided a Bioruptor® Pico demo instrument to us for 2 weeks in September. We were getting very reproducible results between different tubes in the same sonication as well as between replicate experiments. We have obtained considerably improved results especially  for cross-linked chromatin from mammalian cells that has always been a problem for us with the old Bioruptor. A bonus also was a reduced sonication time, although the preparation time is slightly delayed due to the glutinous nature of the beads.

    Dr. Tatyana Nesterova, Department of Biochemistry, University of Oxford

    The new Bioruptor® Pico machine has reduced the amount of time spent sonicating Chromatin by a massive amount. Some protocols require quite harsh fixing conditions which meant fragmenting DNA on the old machine was taking many rounds and several times. With the new Bioruptor® Pico machine these sonications were taking just one round of 10 cycles thereby reducing the fragmentation time substantially. Following sonication, I have used the new IDeal ChIP-seq kit. This is a nice straight forward kit that if followed with an appropriate chip validated antibody gave amazing chip-seq results that worked time and again with several different transcription factors. I would recommend both kits for good, consistant chromatin work.

    Dr. Karen Dawson, RNA Biology Group, Cancer Research UK Manchester Institute at the University of Manchester

    The Bioruptor® NGS* is a robust user-friendly sonication system that is key for both reproducible chromatin shearing and DNA shearing. The Bioruptor® NGS consistently delivered optimal DNA shearing that was critical in generating homogeneous sequencing libraries for our experiments. We also observed excellent chromatin shearing result that were crucial for our epigenetic mapping projects.

    Dr. Sascha Tierling, Department of Genetics and Epigenetics, Prof . Dr. Jörn Walter's lab, Universität des Saarlandes, Germany.
  •  Protocols
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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    Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
    This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...
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    Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
    HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleu...
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    Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
    Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin Shearing...
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    Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
    The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
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    Deparaffinization - Procedure for FFPE nucleic acid extraction with the Bioruptor®
    Deparafinization of FFPE samples is typically performed using a non-polar solvent, such as xylene...
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    DNA Shearing for Bioruptor® Pico - Standard 100 μl Volume
    Standard protocols for programmable DNA size distributions, excellent reproducibility, and high d...
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    DNA Shearing for Bioruptor® Pico - 50 μl Volume
    For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptor...
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    DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
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    DNA Shearing with Bioruptor® Pico - Small Volume (10 µl)
    For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. ...
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    FFPE DNA extraction protocol using the Bioruptor®
    The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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    iST Sample Preparation with the Bioruptor®
    The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e...
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    PCR amplicons shearing using the Bioruptor Pico
    With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR ...
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    Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
    Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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    Re-shearing of decrosslinked immunoprecipitated DNA using the Bioruptor® Pico
    ChIP-seq is a common tool used to study protein-DNA interactions in cells and tissues on a genome...
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    RNA Shearing for Bioruptor® NGS and Pico
    The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, how...
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    Ultrasensitive proteomics for microdissected fresh frozen tissue samples using the Bioruptor® Pico for sample preparation
    Histology-defined proteomics investigations are a fundamental tool in modern molecular pathology....
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  • Available chromatin shearing kits

    It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.

    Chromatin Shearing Kit Low SDS (for Histones) Chromatin Shearing Kit Low SDS (for TF) Chromatin Shearing Kit High SDS Chromatin Shearing Kit (for Plant)

    SDS concentration

    < 0.1%

    0.2%

    1%

    0.5%

    Nuclei isolation

    Yes

    Yes

    No

    Yes

    Allows for shearing of... cells/tissue

    100 million cells

    100 million cells

    100 million cells

    up to 25 g of tissue

    Corresponding to shearing buffers from

    iDeal ChIP-seq kit for Histones

    ChIPmentation Kit for Histones

    iDeal ChIP-seq Kit for Transcription Factors

    iDeal ChIP qPCR kit

    True MicroChIP kit

    Universal Plant
    ChIP-seq kit

  •  Documents
    Bioruptor® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
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    Bioruptor® DNA shearing for Agilent SureSelect library preparation: a perfect combination APPLICATION NOTE
    The Cologne Center for Genomics (CCG), headed by Prof. Dr. Peter Nürnberg, shows that t...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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    Bioruptor® NGS: Unbiased DNA shearing for Next-Generation Sequencing CASE STUDY
    Wouter Coppieters, PhD, and his colleagues operate the genomics core facility at the GIGA center,...
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    True MicroChIP and MicroPlex kits APPLICATION NOTE
    From minuscule amounts to magnificent results: reliable ChIP-seq data from 10,000 cells with the ...
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    ChIP-seq from human tumor tissue APPLICATION NOTE
    Application of the Bioruptor® Pico for shearing chromatin fragments for ChIP-seq from human t...
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    ChIP kit results with True MicroChIP kit POSTER
    Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become the g...
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    Bioruptor® NGS System – Designed Exclusively for Fast & Accurate DNA Shearing POSTER
    The Bioruptor®NGS is the DNA shearing device of choice for sequencing applications, providing...
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    Application of the Bioruptor® Pico for best shearing of yeast genomic DNA for NGS APPLICATION NOTE
    DNA shearing is important step for next-generation sequencing library preparation. Important is r...
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    DNA shearing for Next-Generation Sequencing with the Bioruptor® pico, 0.2 ml Bioruptor® microtubes and the corresponding holder PROTOCOL
    Optimal data generation using NGS platforms relies on a few sample-preparation prerequisites, amo...
    Download
    Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder PROTOCOL
    The first critical step of a successful ChIP experiment is the preparation of sheared chromatin w...
    Download
    Workflow for FFPE-derived DNA shearing prior to Next Generation Sequencing APPLICATION NOTE
    Formalin fixation and paraffin embedding (FFPE) is a standard method for long-term preservation o...
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    Efficient and streamlined solution for high-quality NGS library preparation using the Bioruptor® Pico and JetSeqTM Flex DNA Library Preparation Kit APPLICATION NOTE
    Next Generation Sequencing workflows include a number of critical steps which can have a signific...
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    Comparison of the S. cerevisae chromatin shearing with Covaris™ S2 and Bioruptor® Pico APPLICATION NOTE
    The chromatin shearing is very important step for all kind of ChIP analysis followed by next-gene...
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    Ultrasensitive proteomics for microdissected fresh frozen tissue samples APPLICATION NOTE
    This protocol, specifically designed for ultrasensitive proteomics, enables high throughput quant...
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    Bioruptor® Pico Sonication System Manual MANUAL
    The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as...
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    Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology APPLICATION NOTE
    Dr. Kelvin Luk’s lab at the University of Pennsylvania focuses on understanding Parkinson&r...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Bioruptor® Pico sonication device (Diagenode Cat# B01060010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Corticosteroid receptors adopt distinct cyclical transcriptional signatures
    Florian Le Billan, Larbi Amazit, Kevin Bleakley, Qiong-Yao Xue, Eric Pussard, Christophe Lhadj, Peter Kolkhof, Say Viengchareun, Jérôme Fagart, and Marc Lombès
    Mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs) are two closely related hormone-activated transcription factors that regulate major pathophysiologic functions. High homology between these receptors accounts for the crossbinding of their corresponding ligands, MR being activated by both aldostero...

    BCG Vaccination Protects against Experimental Viral Infection in Humans through the Induction of Cytokines Associated with Trained Immunity.
    Arts RJW, Moorlag SJCFM, Novakovic B, Li Y, Wang SY, Oosting M, Kumar V, Xavier RJ, Wijmenga C, Joosten LAB, Reusken CBEM, Benn CS, Aaby P, Koopmans MP, Stunnenberg HG, van Crevel R, Netea MG
    The tuberculosis vaccine bacillus Calmette-Guérin (BCG) has heterologous beneficial effects against non-related infections. The basis of these effects has been poorly explored in humans. In a randomized placebo-controlled human challenge study, we found that BCG vaccination induced genome-wide epigenetic repr...

    Modulation of p53 and met expression by Krüppel-like factor 8 regulates zebrafish cerebellar development.
    Tsai MY, Lu YF, Liu YH, Lien HW, Huang CJ, Wu JL, Hwang SL
    Krüppel-like factor 8 (Klf8) is a zinc-finger transcription factor implicated in cell proliferation, and cancer cell survival and invasion; however, little is known about its role in normal embryonic development. Here, we show that Klf8 is required for normal cerebellar development in zebrafish embryos. Morphol...

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