Diagenode

Epigenetics Chromatin Analysis

ChIP-qPCR

Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.



  1. Chromatin preparation: cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.
  2. Chromatin shearing: fragmentation of chromatin by sonication down to desired fragment size (100-500 bp)
  3. Chromatin IP: protein-DNA complexe capture using specific ChIP-grade antibodies against the histone or transcription factor of interest
  4. DNA purification: chromatin reverse cross-linking and elution followed by purification
  5. qPCR and analysis: using previously designed primers to amplify IP'd material at specific loci

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

Made for ChIP-qPCR

Cat. No.ProductFormatPrice
C01010171 Auto iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns $1,995.00
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns $1,995.00
C01010153 Auto Universal Plant ChIP-seq kit
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns $495.00
C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-li...
600 µl $125.00
C01010180 iDeal ChIP-qPCR kit
Diagenode’s iDeal ChIP-qPCR kit is a highly optimized solution for ChIP-qPCR assays. The kit provides high yields with excellent specificity and sensit...
24 rxns $480.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C01010130 True MicroChIP Kit
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. T...
16 rxns $495.00
C01010152 Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ...
24 rxns $495.00
B01020001 Bioruptor® Plus sonication device
The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...
1 unit
C03030001 DNA co-precipitant
This IP grade product is optimized to be used as carrier for DNA purification after chromatin immunoprecipitation (ChIP) and after methylated DNA immunopreci...
100 µl $30.00
C03030002 DNA precipitant
This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DN...
1 ml $25.00
C03080030 DNA purifying slurry
The DNA purifying slurry can be used for easy and fast DNA purification and provides you with DNA suitable for qPCR analysis. If you need DNA of higher purit...
10 ml $145.00
C01010120 Elution Module
Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.
1 unit $125.00
C01010063 HighCell# ChIP kit protein G
The Diagenode HighCell# ChIP kit protein G contains protein G-coated paramagnetic beads and negative IgG from mouse. This kit is recommended to perform ChIP ...
48 rxns $1,235.00
C01010062 HighCell# ChIP kit protein A
The Diagenode HighCell# ChIP kit protein A contains protein A-coated paramagnetic beads and negative IgG from rabbit. This kit is recommended to perform ChIP...
48 rxns $1,235.00
C01010100 Histone ChIP kit
Perform robust ChIP (Chromatin Immunoprecipitation) on 100.000 cells per IP. *Number of cells that can be lysed and sheared with this kit.
18 rxns $270.00
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $240.00
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $240.00
C01010072 LowCell# ChIP kit protein A
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns $960.00
C01010073 LowCell# ChIP kit protein G
The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 100...
48 rxns $960.00
C17040004 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl $30.00
C17040005 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl $30.00
C09010010 MethylTaq DNA polymerase
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...
250 units $215.00
C03040001 MicroChIP DiaPure columns
Diagenode's MicroChIP DiaPure columns are ideal in combination with the True MicroChIP kit allowing for the purification and elution of ChIP'd DNA in volumes...
50 rxns $95.00
C01010080 OneDay ChIP kit
Perform ChIP (Chromatin Immunoprecipitation) on large cell numbers. Check out our ChIP-Seq grade antibodies
60 rxns $330.00
C15410048 p53K372me1 polyclonal antibody - Classic
Alternative names: TP53, P53, TRP53, LSF1 Polyclonal antibody raised in rabbit against human p53 (tumor protein p53), methylated at K372, using a KLH-conjug...
50 µg/25 µl $295.00
C17040011 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl $30.00
C17040010 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl $30.00
C17040008 Rice seedlings OsChr4-reg9 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from Rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly t...
50 µl $30.00
C17040009 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl $30.00
C17040006 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $30.00
C17040007 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $30.00
C01010090 Transcription ChIP Kit
The Transcription ChIP kit will be soon discontinued. The last kits are still available - please, contact us if you are interested in this kit. Perform robu...
18 rxns $325.00

Events

  • Epigenetic Mechanisms in Health and Disease 2017
    Barcelona, Spain
    Oct 25-Oct 26, 2017
  • 35ème Congrès de la Societé Française de Toxicologie Génétique
    Marseille, France
    Oct 26-Oct 27, 2017
  • 7th Australian Epigenetics Conference
    Brisbane, Australia
    Oct 29-Nov 1, 2017
  • Australian Genomic Technologies Association (AGTA 2017 Annual Conference)
    Hobart, Tasmania
    Oct 29-Nov 1, 2017
 See all events

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