Diagenode

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  • Shearing Technologies

    Exceptional shearing and sonication of biological samples for any field of study Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more. We offer three types of sonication/shearing devices to match your exact needs for your applic... Learn more

    Bioruptor®

    Bioruptor ultrasonicators with adaptive cavitation technology for highest shearing performance The Diagenode Bioruptor is a state-of-the-art sonication system, cited in more than 2,000 publications. The Bioruptor can simultaneously process up to 12 samples with volume flexibility from 0.5 ml to 50 ml to accurat... Learn more

    Megaruptor®

    Long reads is key for the de novo sequencing, the detection of structural variant and the single molecule sequencing. The Megaruptor® was designed to provide researchers with a simple, automated, and highly reproducible device for the fragmentation of DNA from 3kb - 75kb. Shearing performance is independent of... Learn more

    One®

    The One is the desktop solution for DNA shearing. Learn more

  • Automation

    Diagenode offers a variety of end-to-end systems to streamline DNA methylation and chromatin immunoprecipitation workflows. Diagenode’s IP-Star® Compact is a simple, automated bench-top instrument that standardizes different epigenetics applications (i.e. ChIP, MeDIP or NGS Library preparation) and library preparation. The platform offers full workflow support for epigenetics research, utilizing our complete kits and laboratory-validated protocols to rapidly deliver high-quality and consistent data.  View more TESTIMONIAL Neonatal screening cards provide a valuable bio-b... Learn more

    IP-Star® Compact

    Walk-away automated system Magnetic-bead technology for excellent reproducibility Open platform allows changes in protocol parameters Simple touch screen interface Minimal footprint Processing up to 16 samples Protocols optimal for 100 µl or 200 µl volumes Automated r... Learn more

  • Semi-automation
  • Cell culture

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    3D cell culture plates

    Higher physiological relevance with 3D cell culture plates ChIP-seq results from 3D cell culture showed equivalent reproducibility to 2D cell culture for H3K4me3 and H3K27ac. However, 3D cell culture is advantageous over 2D cell culture with: Better enrichment Higher physiological relevance from spheroid cells ... Learn more

    HeLa-histone cell lines

    HeLa Cell line stably expressing FLAG-HA tagged histones Save time, effort and money thanks to our new HeLa-histone tagged cell lines ! New! Validated HeLa cell lines stably expressing N-terminal FLAG-HA tagged human histone for: Functional gene analysis Cellular localization Target identification Isolat... Learn more

  • Genome editing (CRISPR/Cas9)

    CRISPR/Cas9 The CRISPR/Cas genome editing system uses a RNA-guided endonuclease to introduce site-directed double-strand breaks (DSBs) in DNA. The endonuclease Cas9 can be used for DNA cleavage at specific sites defined by guide RNAs and has been adopted as a technology of choice for genome engineering. Cas9-mediated cleavage relies on the presence of a protospacer adjacent motif (PAM) in the target DNA. The Non Homologous End Joining (NHEJ) DNA repair pathway plays a role in repairing Cas9-induced DSBs. As the repair is prone to errors, insertions and deletions (indels) may occur that subsequently disrupt gene function. DSBs m... Learn more

    Cas9 Nuclease protein

    The CRISPR/Cas genome editing system consists of a “guide” RNA (gRNA) and the Cas9 endonuclease. The gRNA is comprised of a synthetic RNA with a “scaffold” sequence to which Cas9 binds as well as a user-selected ∼20 nucleotide “targeting” sequence for the genomic target to be ... Learn more

  • Kits

    Diagenode’s epigenetic reagents include: DNA methylation kits and antibodies - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3. ChIP and ChIP-seq kits for industry-leading specificity and sensitivity - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification r... Learn more

    クロマチン機能

    Diagenodeはクロマチン研究を容易に、またアクセスしやすくするための多数のユニークなソリューションとキットを提供しています。 当社のクロマチン免疫沈降キットは、植物や哺乳類のヒストン、転写因子、低細胞開始インプットの研究など、幅広い研究ニーズに対応するように設計されています。 また、クロマチン免疫沈降のための手動、半自動、完全自動のソリューションを取り揃えています。 Learn more

    DNA methylation

    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers products for three main appr... Learn more

    DNA/RNA purification

    Diagenode offers a variety of optimized DNA purification methods allowing for recovery of high quality DNA after chromatin immunoprecipitation or methylated DNA immunoprecipitation, essential for ideal results in downstream applications such as qPCR or next generation sequencing. Our purification technology in o... Learn more

    Exosomes

    The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that exosomes, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including ... Learn more

    DNA/RNA library preparation

    Diagenode provides a complete portfolio of library preparation kits for Next-Generation Sequencing, covering RNA-seq, ChIP-seq, and DNA sequencing.  Our library preparation solutions can be used for low inputs, from as little as 10 pg of starting material. Our kits offer simple and fast workflows, high yiel... Learn more

    Protein Extraction

    Diagenode offers optimized protein extraction kits for use with the Bioruptor® sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor®. Learn more

  • Antibodies

    Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of your ChIP and ChIP-seq experiments, so we emphasize strict quality standards and actual validation for all of our antibodies. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every ChIP assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-s... Learn more

    All antibodies

    All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (requires less antibody per reaction) Batch-specific data is av... Learn more

    New antibodies

    Diagenode’ selection of antibodies is exclusively dedicated for epigenetic research. Our R&D is continually working on the development of novel antibodies of the highest quality. Have a look at our latest antibodies. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-e... Learn more

    Sample size antibodies

    Validated epigenetics antibodies – care for a sample?  Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-spe... Learn more

    Premium Antibodies

    The scientific community trusts Diagenode’s antibodies BLUEPRINT, a part of the IHEC consortium is a large-scale research project which involves 42 leading European universities, research institutes, and industry entrepreneurs. The project aims to generate 100 reference epigenomes of distinct ty... Learn more

    ChIP-seq grade antibodies

    Unparalleled ChIP-Seq results with the most rigorously validated antibodies Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, ... Learn more

    ChIP-grade antibodies

    Chromatin immunoprecipitation (ChIP) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies us... Learn more

    Chromatin associated proteins

    Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chro... Learn more

    Histone modifying enzymes

    Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (re... Learn more

    CRISPR / Cas9抗体

    CRISPR / Casゲノム編集システムは、RNA依存性エンドヌクレアーゼ技術を用いて、ゲノムの任意の位置でのインデル変異、特定のシーケンス置換、挿入および大きな欠失、ゲノム再編成を誘導します。.さらに、この技術により特定の内因性遺伝子のアップレギュレーションまたはダウンレギュレーションの仲介、ヒストン修飾またはDNAメチル化を変更が可能になります。最も知られているCRISPR関連ヌクレアーゼは、Streptococcus pyogenes(化膿レンサ球菌)およびStaphylococcus aureus(黄色ブドウ球菌)由来のCas9タンパク質です。最近、Cpf1(Lachnospiraceae細菌およびAcidamino... Learn more

    Isotype controls

    Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse.  These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively. Diagenode’s highly validated antibodies: H... Learn more

    DNA modifications

    The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylat... Learn more

    Histone antibodies

    Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are l... Learn more

    Transcription

    The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors.  Check the list below to see our targets. Diagenode’s highly validated antibodies:         Highly sen... Learn more

    Exclusive customized epigenetic antibodies

    For many years, Diagenode has acquired a strong and reliable experience in the production of custom epigenetic antibodies, allowing us to become one of the most reliable partner in several EU projects in this field of studies. Following our success in optimizing and developing our epigenetic antibody production proc... Learn more

  • Reagents

    Diagenode offers full and validated solutions for every step of your epigenetics sample preparation, making epigenetics research easy, accessible, and reliable. Beside our complete kit solutions please find here below an exhaustive list of all high quality reagents realated to your epigenetics research. Learn more

    Proteins

                                           Diagenode now offers a broad range of tools to study nucleosomes and histones, the structural and functional units of chromatin. We provide solutions for any architectural l... Learn more

    Beads and accessories

    DiaMag beads are intended for isolation of immune complexes in ChIP experiments or MeDIP experiments and have been extensively validated for these assays. The beads are compatible for immunoprecipitation of a number of IgGs and polyclonal antibodies across a number of species.   Learn more

    Primer pairs

    Our primer pairs have been carefully designed for high specificity to specific genomic regions. Primers have been tested and optimized to be used in a number of applications including DNA amplification after ChIP, ChIP-seq library validation, qPCR, and RT-qPCR.   Learn more

    Plasmids

    Our yeast low copy BirA expression plasmids are designed for in vivo yeast biotinylation systems for sensitive detection of protein-DNA complexes using chromatin immunoprecipitaion. The constructs include a nuclear localisation signal. We also offer biotinylation tagging cassettes for C- and N- terminal tagging. Learn more

  • Epigenomics Profiling Services

    Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.  CHROMATIN analysis Histone modification ChIP-seq analysis Promoter analysis Enhancer analysis Transcription factor ChIP-seq analysis Customized NGS s... Learn more

    RNA-seq

    RNA sequencing uses next-generation sequencing (NGS) to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome. Diagenode utilizes the unique capture and amplification by tailing and switching (CATS) methodology for generating RNAseq libraries which is optimal for low o... Learn more

    DNAメチル化

    DNAメチル化およびヒストン修飾のパターンは、細胞機能を維持する上で不可欠な役割を果たします。 異常なDNAメチル化 - 過剰メチル化および低メチル化 - は、癌または他の疾患のような有害転帰をもたらし得ます。ゲノムワイドDNAメチル化分析による5-mCの定量化は、疾患の検出および予後のための情報を提供することが可能です。 DiagenodeのDNAメチル化解析サービスは、あらゆる種のゲノムDNAメチル化解析の標的となる全ゲノムDNAメチル化解析用の多数の重亜硫酸塩シークエンシングオプションをご提供いたします。 表現の重亜硫酸塩シークエンシング(RRBS RRBSは、ゲノム全体の重亜硫酸塩シークエン... Learn more

    クロマチン プロファイリング サービス

    クロマチンは、DNA、ヒストンおよび非ヒストンタンパク質からなります。 クロマチンを形成する際に、ヒストンの周りはDNAにしっかりと包まれています。 一般に、より高度に濃縮されたクロマチンが、転写因子や他のDNA結合タンパク質に接近することは殆どなく、遺伝子発現に影響します。 ヒストンおよび転写因子の役割を理解することは、遺伝子発現の調節を理解する上で非常に重要です。 ChIP-seq分析を用いて、ゲノム全体にわたるヒストン修飾および転写因子結合をプロファイルし、疾患または薬物治療に反応する遺伝子発現の調節を理解することができます。 Diagenodeのエピゲノムプロファイリングサービスは、ChIP-seqおよび... Learn more

  • Real time PCR master mix

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