Categories

Shearing technologies

Exceptional shearing and sonication of biological samples for any field of study Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more. We offer three types of sonication/shearing devices to match your exact needs for your applic... Learn more

Bioruptor^®

Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, ... Learn more

Megaruptor^®

Your partner in long-read sequencing Sequencing technologies have revolutionized genomics and biology researches. Long read sequencing enables researchers to access a more comprehensive view of genomes with higher accuracy. However, one of the most critical aspects of optimal library preparation is the quality of... Learn more

One^®

The One is the desktop solution for DNA shearing. Learn more

Maintenance Plans

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Tagmentation

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Chromatin studies

Chromatin structure plays a key role in regulating gene expression by allowing DNA accessibility to transcriptional machinery and transcription factors. The packaging of DNA into nucleosomes forms a closed structure that is not highly accessible to transcriptional elements whereas the open nucleosome structure allows DNA to be accessible. Diagenode offers a number of solutions to help you analyze chromatin and the role of transcriptional machinery including ChIP kits, ChIPmentation kits, antibodies, pA-Tn5 and ATAC-seq kits. // = 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write(''); } else { documen... Learn more

Chromatin shearing

The first step of ChIP - chromatin preparation - plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample ... Learn more

Chromatin immunoprecipitation

ChIP, Chromatin Immunoprecipitation, is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This powerful method offers insight into the regulation of gene expression. This technique is now used in a variety of life science disciplines including cellul... Learn more

ChIPmentation™

ChIPmentation for high-quality and fast ChIP-seq library preparation Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that re... Learn more

CUT&Tag

CUT&Tag-sequencing (Cleavage Under Targets and Tagmentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete so... Learn more

ATAC-seq

ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the transposase Tn5 which cuts exposed open chromatin and simultaneously ligates adapters for subsequent ampli... Learn more

DNA methylation

Understanding DNA methylation is critical for learning about control of transcription, genomic imprinting, embryonic development, and carcinogenesis. Diagenode proposes two main approaches to studying DNA methylation - bisulfite conversion with downstream sequencing or qPCR and immunoprecipitation (IP). Diagenode Product Solutions Study methylation with single base-resolution Does sequencing cost play a role? NO YES Premium WGBS Kit Premium RRBS Kit Generate bisulfite converted gDNA libraries ready for gDNA single or paired-end sequencing of the whole genome Generate bisulfite co... Learn more

Bisulfite conversion

Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing single nucleotide resolution. It enables to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by PCR amplification or next g... Learn more

Methylated DNA immunoprecipitation

The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is per... Learn more

Methylbinding domain protein

The MBD technology used in our MethylCap kit is based on the very high affinity of the MethylCap protein for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. Dia... Learn more

DNA methylation standard

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PCR enzymes and nucleotides

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Hydroxymethylated DNA immunoprecipitation

The hydroxymethylated DNA IP (hMeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-hydroxymethylcytosine (5-hmC). How it works In brief, hydroxymethyl DNA IP is performed as follows: starting from sheared genomic DNA from cultured cells or tissues, the immun... Learn more

Cell culture

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Genome editing (CRISPR/Cas9)

CRISPR/Cas9 The CRISPR/Cas genome editing system uses a RNA-guided endonuclease to introduce site-directed double-strand breaks (DSBs) in DNA. The endonuclease Cas9 can be used for DNA cleavage at specific sites defined by guide RNAs and has been adopted as a technology of choice for genome engineering. Cas9-mediated cleavage relies on the presence of a protospacer adjacent motif (PAM) in the target DNA. The Non Homologous End Joining (NHEJ) DNA repair pathway plays a role in repairing Cas9-induced DSBs. As the repair is prone to errors, insertions and deletions (indels) may occur that subsequently disrupt gene function. DSBs m... Learn more

Selection of guide RNA by ChIP

While a number of computational tools help design sgRNAs to target specific loci, the accurate prediction of whether Cas9 and the sgRNA of interest will bind specifically to the genome is still a challenge. In order to ensure specific targeting of CRISPR/Cas9, the verification of the specific binding of the sgRNA a... Learn more

Kits

Diagenode’s epigenetic reagents include: DNA methylation kits and antibodies - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3. ChIP and ChIP-seq kits for industry-leading specificity and sensitivity - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification r... Learn more

Antibodies

Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We are committed to develop and validate the antibodies in epigenetic applications for chromatin studies, like ChIP, ChIP-seq, CUT&Tag, etc. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every ChIP, ChIP-seq and CUT&Tag assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-specific vali... Learn more

All antibodies

All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (requires less antibody per reaction) Batch-specific data is av... Learn more

Sample size antibodies

Validated epigenetics antibodies – care for a sample? Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-spe... Learn more

ChIP-seq grade antibodies

Unparalleled ChIP-Seq results with the most rigorously validated antibodies Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, ... Learn more

ChIP-grade antibodies

Chromatin immunoprecipitation (ChIP) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies us... Learn more

Western Blot Antibodies

We have what you need for a successful Western blot!Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly.For a successful Western blot we offer:Loading c... Learn more

Chromatin associated proteins

Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chro... Learn more

Histone modifying enzymes

Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (re... Learn more

Isotype controls

Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively. Diagenode’s highly validated antibodies: H... Learn more

DNA modifications

The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylat... Learn more

RNA associated proteins

Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated protein... Learn more

RNA modifications

Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies be... Learn more

Histone antibodies

Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are l... Learn more

CRISPR/Cas9 antibodies

The CRISPR/Cas genome editing system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or ... Learn more

CUT&Tag Antibodies

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Transcription

The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets. Diagenode’s highly validated antibodies: Highly sen... Learn more

Epitope tags

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Other antibodies

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NGS Library preparation

Diagenode provides a complete portfolio of library preparation kits for Next-Generation Sequencing, covering RNA-seq, ChIP-seq, and DNA sequencing. Our library preparation solutions can be used for low inputs, from as little as 10 pg of starting material. Our kits offer simple and fast workflows, high yields, and ready-to-sequence DNA. Our solutions incorporate user-friendly protocols that even beginners can easily master upon the first use. Learn more

Library preparation for ChIP-seq

Library preparation following ChIP can be challenging due to the limited amount of DNA recovered after ChIP. Diagenode has developed the optimal solutions for ChIP-seq using two different approaches: the ligation-based library preparation on purified DNA or the tagmentation-based ChIPmentation. ... Learn more

RNA library preparation

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNA... Learn more

Library preparation for DNA sequencing

Diagenode offers innovative DNA library preparation solutions such as a hyperactive tagmentase and the “capture and amplification by tailing and switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of DNA. Our powerfull ChIP-seq library ... Learn more

Epigenomics Profiling Services

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Automation

Sales of the IP-Star Compact instrument have been discontinued. Supporting materials, repairs, and consumables will still be available Supporting Materials Documents Antibody ChIPSeq QC using the IPStar Compact POSTERChromatin immunoprecipitation (ChIP) is the most widely used method to study protein-DNA interact... Download High Resolution ChIPSeq Profiles with IPStar Automated Platform POSTERChromatin immunoprecipitation followed by next generation sequencing (ChIP-Seq) is a powerful tec... Download Automated method for RNAseq libraries APPLICATION NOTEAn automated method for efficient, accura... Learn more

IP-Star accessories

The plastics have been selected and validated together with the IP-Star Compact® automated system for an optimal performance. Learn more

Real time PCR master mix

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Reagents

Diagenode offers full and validated solutions for every step of your epigenetics sample preparation, making epigenetics research easy, accessible, and reliable. Beside our complete kit solutions please find here below an exhaustive list of all high quality reagents related to your epigenetics research. Learn more

Beads, magnetic racks and accessories

DiaMag beads and magnetic racks are intended for isolation of immune complexes in ChIP experiments or MeDIP experiments and have been extensively validated for these assays. The beads are compatible for immunoprecipitation of a number of IgGs and polyclonal antibodies across a number of species. Learn more

DNA/RNA purification

Diagenode offers a variety of optimized DNA purification methods allowing for recovery of high quality DNA after chromatin immunoprecipitation or methylated DNA immunoprecipitation, essential for ideal results in downstream applications such as qPCR or next generation sequencing. Our purification technology in o... Learn more

Fixation reagents

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Enzymes

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Inhibitors

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Primer indexes for tagmented libraries

The Primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by tagmentation. Each primer index is baring a unique index in order to identify each library before pooling them for sequencing in the same lane. They are compatible with an... Learn more

Primer pairs

Our primer pairs have been carefully designed for high specificity to specific genomic regions. Primers have been tested and optimized to be used in a number of applications including DNA amplification after ChIP, ChIP-seq library validation, qPCR, and RT-qPCR. Learn more

Protein Extraction

Diagenode offers optimized protein extraction kits for use with the Bioruptor® sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor®. Learn more

Proteins

The below list shows the peptides used as immunogen to raise some of Diagenode antibodies. These peptides can be used to block the corresponding antibody in negative control... Learn more

Exosomes

The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that exosomes, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including ... Learn more

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