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Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, ... Learn more

Your partner in long-read sequencing Sequencing technologies have revolutionized genomics and biology researches. Long read sequencing enables researchers to access a more comprehensive view of genomes with higher accuracy. However, one of the most critical aspects of optimal library preparation is the quality of... Learn more

The One is the desktop solution for DNA shearing. Learn more

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The first step of ChIP - chromatin preparation - plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample ... Learn more

ChIP, Chromatin Immunoprecipitation, is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This powerful method offers insight into the regulation of gene expression. This technique is now used in a variety of life science disciplines including cellul... Learn more

ChIPmentation for high-quality and fast ChIP-seq library preparation Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that re... Learn more

CUT&Tag-sequencing (Cleavage Under Targets and Tagmentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete so... Learn more

ATAC-seq, Assay for Transposase-Accessible Chromatin, followed by next generation sequencing, is a key technology for genome-wide mapping of accessible chromatin. The technology is based on the use of the transposase Tn5 which cuts exposed open chromatin and simultaneously ligates adapters for subsequent ampli... Learn more

Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing single nucleotide resolution. It enables to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by PCR amplification or next g... Learn more

The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is per... Learn more

The MBD technology used in our MethylCap kit is based on the very high affinity of the MethylCap protein for methylated DNA. This protein consists of the methyl-CpG-binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag.  Dia... Learn more

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The hydroxymethylated DNA IP (hMeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-hydroxymethylcytosine (5-hmC). How it works In brief, hydroxymethyl DNA IP is performed as follows: starting from sheared genomic DNA from cultured cells or tissues, the immun... Learn more

While a number of computational tools help design sgRNAs to target specific loci, the accurate prediction of whether Cas9 and the sgRNA of interest will bind specifically to the genome is still a challenge. In order to ensure specific targeting of CRISPR/Cas9, the verification of the specific binding of the sgRNA a... Learn more

All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (requires less antibody per reaction) Batch-specific data is av... Learn more

Validated epigenetics antibodies – care for a sample?  Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-spe... Learn more

Unparalleled ChIP-Seq results with the most rigorously validated antibodies Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, ... Learn more

Chromatin immunoprecipitation (ChIP) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies us... Learn more

We have what you need for a successful Western blot!Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly.For a successful Western blot we offer:Loading c... Learn more

Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chro... Learn more

Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases. Diagenode’s highly validated antibodies: Highly sensitive and specific Cost-effective (re... Learn more

Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse.  These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively. Diagenode’s highly validated antibodies: H... Learn more

The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylat... Learn more

Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated protein... Learn more

Diagenode’s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies be... Learn more

Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are l... Learn more

The CRISPR/Cas genome editing system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or ... Learn more

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The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors.  Check the list below to see our targets. Diagenode’s highly validated antibodies:         Highly sen... Learn more

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Library preparation following ChIP can be challenging due to the limited amount of DNA recovered after ChIP. Diagenode has developed the optimal solutions for ChIP-seq using two different approaches: the ligation-based library preparation on purified DNA or the tagmentation-based ChIPmentation. ... Learn more

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNA... Learn more

Diagenode offers innovative DNA library preparation solutions such as a hyperactive tagmentase and the “capture and amplification by tailing and switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of DNA. Our powerfull ChIP-seq library ... Learn more

The plastics have been selected and validated together with the IP-Star Compact® automated system for an optimal performance. Learn more

DiaMag beads and magnetic racks are intended for isolation of immune complexes in ChIP experiments or MeDIP experiments and have been extensively validated for these assays. The beads are compatible for immunoprecipitation of a number of IgGs and polyclonal antibodies across a number of species.   Learn more

Diagenode offers a variety of optimized DNA purification methods allowing for recovery of high quality DNA after chromatin immunoprecipitation or methylated DNA immunoprecipitation, essential for ideal results in downstream applications such as qPCR or next generation sequencing. Our purification technology in o... Learn more

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Our yeast low copy BirA expression plasmids are designed for in vivo yeast biotinylation systems for sensitive detection of protein-DNA complexes using chromatin immunoprecipitaion. The constructs include a nuclear localisation signal. We also offer biotinylation tagging cassettes for C- and N- terminal tagging. Learn more

The Primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by tagmentation. Each primer index is baring a unique index in order to identify each library before pooling them for sequencing in the same lane. They are compatible with an... Learn more

Our primer pairs have been carefully designed for high specificity to specific genomic regions. Primers have been tested and optimized to be used in a number of applications including DNA amplification after ChIP, ChIP-seq library validation, qPCR, and RT-qPCR.   Learn more

Diagenode offers optimized protein extraction kits for use with the Bioruptor® sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor®. Learn more

                                       The below list shows the peptides used as immunogen to raise some of Diagenode antibodies. These peptides can be used to block the corresponding antibody in negative control... Learn more

The identification of reliable predictive or diagnostic biomarkers is crucial for biomedical research. Recent evidence shows that exosomes, small vesicles that are excreted into the extracellular environment, are excellent disease biomarker candidates as they contain specific biological material including ... Learn more