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ChIPmentation Kit for Histones

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Catalog Number
Format
Price
C01011010
24 rxns
$2,320.00

Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation. Using histone ChIPmentation, you can avoid the multi-step ligation required for traditional histone ChIP-seq. ChIPmentation enables an easier protocol, faster time to results, and excellent reproducibility for histone ChIP-seq from challenging and low input samples.

  • Characteristics

    ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. ChIPmentation involves much easier and shorter protocol with high efficiency for low input samples using optimized ChIP and NGS sample preparation.


    Benefits of the ChIPmentation system for histone ChIP-seq

    • Easier and faster than classical ChIP-seq
    • Validated for various histone marks
    • Ideal for analysis of large cohorts of samples
    • Ideal for analysis of large number of marks on a unique sample
    • High quality sequencing data

    ChIPmentation - Highest reliability for even the lowest inputs

    The new histone ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.


    Figure 1: ChIPmentation sequencing results obtained from decreasing starting amounts of cells.
    Chromatin preparation has been performed on 7 M  K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011010). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.

    Figure 2: ChIPmentation sequencing results.
    Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit fro Histones. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).

  • Additional solutions compatible with ChIPmentation Kit for Histones

    Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones) optimizes chromatin shearing, a critical step for ChIP.

    ChIP-seq grade anti-histone antibodies provide high yields with excellent specificity and sensitivity.

    For fast and efficient isolation of magnetic beads we recommend the magnetic racks DiaMag1.5 and DiaMag0.2.

     Plus, for our IP-Star Automation users for automated ChIP, check out our automated version of this kit.

  •  Documents
    ChIPmentation for Histones MANUAL
    Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of lib...
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  •  Safety sheets
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ChIPmentation Kit for Histones (Diagenode Cat# C01011010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells
    Tryndyak, V.P., Borowa-Mazgaj, B., Steward, C.R. et al. Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells.
    Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowledge and many unanswered questions remain to explain the integrated mole...

    Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development
    Roels J, Kuchmiy A, De Decker M, et al.
    The development of TCRαβ and TCRγδ T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the conti...

    Measuring Histone Modifications in the Human Parasite Schistosoma mansoni
    de Carvalho Augusto R, Roquis D, Al Picard M, Chaparro C, Cosseau C, Grunau C.
    DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of ge...

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