Diagenode

>>>   Click for Diagenode’s approach to COVID-19

24 UDI for Tagmented libraries - Set I

Catalog Number
Format
Price
C01011034
24 UDI
$170.00
Other format

The 24 UDI for tagmented libraries includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to 24 samples for sequencing on Illumina platforms. These UDI are designed and validated to be used with μChIPmentation for Histones (Cat. No. C01011011), ChIPmentation Kit for Histones (Cat. No. C01011009), TAG Kit for ChIPmentation (Cat. No. C01011030), ATAC-seq Kit (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other tagmentation-based library preparation protocols, such as CUT&Tag technologies.

4 sets of UDI for tagmented libraries are available:

24 UDI for tagemented libraries - Set I
24 UDI for tagmented libraries - Set II
24 UDI fro tagmented libraries -  Set III
24 UDI for tagmented libraries - Set IV

Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 96.

Features:

  • Multiplexing: up to 96 samples (using all 4 sets simultaneously)
  • Allow for identification of index hopping
  • Compatibility: tagmentation-based library preparation protocols

  • Characteristics

    The 24 UDI (Unique dual indexes) for tagmented libraries – Set I is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.

    The 24 UDI for tagmented libraries provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

    Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used.

  •  Documents
    Primer indexes for tagmented libraries MANUAL
    The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapt...
    Download
  •  Safety sheets
    24 UDI for Tagmented libraries - Set I SDS GB en Download
    24 UDI for Tagmented libraries - Set I SDS US en Download
    24 UDI for Tagmented libraries - Set I SDS BE nl Download
    24 UDI for Tagmented libraries - Set I SDS BE fr Download
    24 UDI for Tagmented libraries - Set I SDS FR fr Download
    24 UDI for Tagmented libraries - Set I SDS ES es Download
    24 UDI for Tagmented libraries - Set I SDS DE de Download
    24 UDI for Tagmented libraries - Set I SDS JP ja Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 24 UDI for Tagmented libraries - Set I (Diagenode Cat# C01011034). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  Related products

Events

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics