Diagenode

24 UDI for Tagmented libraries - Set I

Catalog Number
Format
Price
C01011034
24 UDI
$185.00
Other format

The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

The 24 UDI for tagmented libraries – Set I includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to 24 samples for sequencing on Illumina platforms. This set of indexes is designed to be used with μChIPmentation for Histones (Cat. No. C01011011) or TAG Kit for ChIPmentation (Cat. No. C01011030) but it is also compatible with other tagmentation-based library preparation protocols, such as ATAC-seq or CUT&Tag technologies.

  • Characteristics

    The 24 UDI (Unique dual indexes) for tagmented libraries – Set I is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.

    The 24 UDI for tagmented libraries provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

    Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used.

  •  資料
    Primer indexes for tagmented libraries MANUAL
    The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapt...
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  •  Safety sheets
    24 UDI for Tagmented libraries - Set I SDS US en Download
    24 UDI for Tagmented libraries - Set I SDS GB en Download
    24 UDI for Tagmented libraries - Set I SDS ES es Download
    24 UDI for Tagmented libraries - Set I SDS DE de Download
    24 UDI for Tagmented libraries - Set I SDS JP ja Download
    24 UDI for Tagmented libraries - Set I SDS BE nl Download
    24 UDI for Tagmented libraries - Set I SDS BE fr Download
    24 UDI for Tagmented libraries - Set I SDS FR fr Download
  •  出版物

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