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TAG Kit for ChIPmentation

Catalog Number

Format

Price

C01011030

24 rxns

$1,050.00

Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。番号C01011031

TESTIMONIAL
One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).

Region 1

Region 2

Figure 1. ChIPmentation sequencing profiles for Gata6
Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).

Takahiro Suzuki, Ph.D., Senior Research Scientist, Cellular Function Conversion Technology Team, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan

特徴
Comparing to classical ChIP-seq, ChIPmentation enables:
- An easier protocol
- Faster time to results
- Flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with any ChIP protocol (based on magnetic beads)
ChIPmentation on histone marks using TAG KIT for ChIPmentation

ChIPmentation sequencing results for four histone marks. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.

*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).

ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation

ChIPmentation sequencing results for two non histone factors. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).
お客様の声
We were very happy with the method. It gave good results in the end, and required much smaller samples than we need to reliably perform conventional ChIP-seq.
In our view, the main advantages of the ChIPmentation kit compared to our conventional ChIP-seq protocol are (most important first):
- smaller sample requirement,
- simpler workflow with less that can go wrong,
- slightly higher resolution and signal: noise ratio.
ChIPmentation sequencing profiles for p65. Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).

Researcher from University of Nice-Sophia Antipolis, Nice, France
資料
TAG Kit for ChIPmentation MANUAL
Diagenode’s TAG Kit for ChIPmentation in combination with the set of indexes (24 SI for ChI... Download
Auto ChIPmentation for TFs PROTOCOL
Download

出版物

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A dataset of definitive endoderm and hepatocyte differentiations fromhuman induced pluripotent stem cells.
Tanaka Y. et al.
Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation ...

GATA6 is predicted to regulate DNA methylation in an in vitro model ofhuman hepatocyte differentiation.
Suzuki T. et al.
Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is...

関連商品
- C01011031
  
  24 SI for ChIPmentation

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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<li>Flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
		'label1' => '特徴',
		'info1' => '<h3>Comparing to classical ChIP-seq, ChIPmentation enables:</h3>
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<li>An easier protocol</li>
<li>Faster time to results</li>
<li>Flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<li>flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
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<div class="small-12 medium-8 large-8 columns">
<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode&rsquo;s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> &ndash; The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">&micro;ChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> &ndash; <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> &ndash; <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
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<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star&reg; to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star&reg;. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> &micro;ChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode &micro;ChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&amp;Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries &ndash; Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase &ndash; loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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			'description' => '<p>Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation time-course of hepatocyte-like cells. We performed methylome and transcriptome analyses for hepatocyte-like cell differentiation. We also analyzed DE-like cell differentiation by methylome, transcriptome, chromatin accessibility, and GATA6 binding profiles, using finer time-course samples. In this manuscript, we provide a detailed description of the dataset and the technical validations. Our data may be valuable for the analysis of the molecular mechanisms underlying hepatocyte and DE differentiations.</p>',
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			'description' => '<p>Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is regulated, particularly the dynamics of DNA methylation and the critical upstream factors. Here, by examining changes in the transcriptome and the methylome using an in vitro hepatocyte differentiation model, we show putative DNA methylation-regulating transcription factors, which are likely involved in DNA demethylation and maintenance of hypo-methylation in a differentiation stage-specific manner. Of these factors, we further reveal that GATA6 induces DNA demethylation together with chromatin activation in a binding-site-specific manner during endoderm differentiation. These results provide an insight into the spatiotemporal regulatory mechanisms exerted on the DNA methylation landscape by transcription factors and uncover an epigenetic role for transcription factors in early liver development.</p>',
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<li>smaller sample requirement,</li>
<li>simpler workflow with less that can go wrong,</li>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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			'name' => 'From Dr Takahiro Suzuki about iDeal ChIP-seq kit for Transcription Factors, TAG Kit for ChIPmentation, 24 SI for ChIPmentation',
			'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Region 1</strong></small></p>
<center>
<p><img src="../../img/product/kits/chipmentation-gata6-region1.png" /></p>
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<p><small><strong>Region 2</strong></small></p>
<center><img src="../../img/product/kits/chipmentation-gata6-region2.png" /></center></div>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
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<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode&rsquo;s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> &ndash; The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">&micro;ChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> &ndash; <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> &ndash; <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
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<div class="small-12 medium-4 large-4 columns"><center><a href="https://www.citeab.com/awards/2018/innovative-product-of-the-year" target="_blank"><img src="https://www.diagenode.com/emailing/images/citeab.png" width="224" height="200" /></a><br /><br />
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star&reg; to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star&reg;. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> &micro;ChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode &micro;ChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&amp;Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries &ndash; Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase &ndash; loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
</li>
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			'description' => '<p>Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is regulated, particularly the dynamics of DNA methylation and the critical upstream factors. Here, by examining changes in the transcriptome and the methylome using an in vitro hepatocyte differentiation model, we show putative DNA methylation-regulating transcription factors, which are likely involved in DNA demethylation and maintenance of hypo-methylation in a differentiation stage-specific manner. Of these factors, we further reveal that GATA6 induces DNA demethylation together with chromatin activation in a binding-site-specific manner during endoderm differentiation. These results provide an insight into the spatiotemporal regulatory mechanisms exerted on the DNA methylation landscape by transcription factors and uncover an epigenetic role for transcription factors in early liver development.</p>',
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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			'name' => 'From Dr Takahiro Suzuki about iDeal ChIP-seq kit for Transcription Factors, TAG Kit for ChIPmentation, 24 SI for ChIPmentation',
			'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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$testimonials = '<blockquote><p>We were very happy with the method. &nbsp;It gave good results in the end, and required much smaller samples than we need to reliably perform conventional ChIP-seq. <br />In our view, the main advantages of the ChIPmentation kit compared to our conventional ChIP-seq protocol are (most important first):</p>
<ul>
<li>smaller sample requirement,</li>
<li>simpler workflow with less that can go wrong,</li>
<li>slightly higher resolution and signal: noise ratio.</li>
</ul>
<div class="small-12 columns"><center><img src="../../img/product/kits/chipmentation-sequencing-p65.png" /></center></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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'
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Region 1</strong></small></p>
<center>
<p><img src="../../img/product/kits/chipmentation-gata6-region1.png" /></p>
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<div class="small-12 columns">
<p><small><strong>Region 2</strong></small></p>
<center><img src="../../img/product/kits/chipmentation-gata6-region2.png" /></center></div>
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<div class="extra-spaced"></div>
<div class="row">
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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</div><cite>Takahiro Suzuki, Ph.D., Senior Research Scientist, Cellular Function Conversion Technology Team, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan</cite></blockquote>
'
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	'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<div class="small-12 columns">
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<div class="extra-spaced"></div>
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<div class="small-12 columns">
<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>24 SI for ChIPmentation個カートに追加。</p>

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            <h6 style="height:60px">24 SI for ChIPmentation</h6>
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<div align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<ul>
<li>an easier protocol</li>
<li>faster time to results</li>
<li>flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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	'description' => '<p>Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is regulated, particularly the dynamics of DNA methylation and the critical upstream factors. Here, by examining changes in the transcriptome and the methylome using an in vitro hepatocyte differentiation model, we show putative DNA methylation-regulating transcription factors, which are likely involved in DNA demethylation and maintenance of hypo-methylation in a differentiation stage-specific manner. Of these factors, we further reveal that GATA6 induces DNA demethylation together with chromatin activation in a binding-site-specific manner during endoderm differentiation. These results provide an insight into the spatiotemporal regulatory mechanisms exerted on the DNA methylation landscape by transcription factors and uncover an epigenetic role for transcription factors in early liver development.</p>',
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<li>Flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<li>flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
</ul>
<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div class="small-12 medium-8 large-8 columns">
<div id="video"></div>
<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode&rsquo;s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> &ndash; The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">&micro;ChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> &ndash; <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> &ndash; <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
<div class="extra-spaced">
<div id="video" align="center"><video width="400" height="250" autoplay="autoplay" muted="" loop="loop" controls="controls" src="https://www.diagenode.com/videos/chipmentation-dgo.mp4" frameborder="0" allowfullscreen="allowfullscreen"></video></div>
<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star&reg; to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star&reg;. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> &micro;ChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode &micro;ChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&amp;Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries &ndash; Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase &ndash; loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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			'name' => 'A dataset of definitive endoderm and hepatocyte differentiations fromhuman induced pluripotent stem cells.',
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			'description' => '<p>Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation time-course of hepatocyte-like cells. We performed methylome and transcriptome analyses for hepatocyte-like cell differentiation. We also analyzed DE-like cell differentiation by methylome, transcriptome, chromatin accessibility, and GATA6 binding profiles, using finer time-course samples. In this manuscript, we provide a detailed description of the dataset and the technical validations. Our data may be valuable for the analysis of the molecular mechanisms underlying hepatocyte and DE differentiations.</p>',
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			'description' => '<p>Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is regulated, particularly the dynamics of DNA methylation and the critical upstream factors. Here, by examining changes in the transcriptome and the methylome using an in vitro hepatocyte differentiation model, we show putative DNA methylation-regulating transcription factors, which are likely involved in DNA demethylation and maintenance of hypo-methylation in a differentiation stage-specific manner. Of these factors, we further reveal that GATA6 induces DNA demethylation together with chromatin activation in a binding-site-specific manner during endoderm differentiation. These results provide an insight into the spatiotemporal regulatory mechanisms exerted on the DNA methylation landscape by transcription factors and uncover an epigenetic role for transcription factors in early liver development.</p>',
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<ul>
<li>smaller sample requirement,</li>
<li>simpler workflow with less that can go wrong,</li>
<li>slightly higher resolution and signal: noise ratio.</li>
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<div class="small-12 columns"><center><img src="../../img/product/kits/chipmentation-sequencing-p65.png" /></center></div>
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<div class="small-12 columns">
<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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			'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Region 1</strong></small></p>
<center>
<p><img src="../../img/product/kits/chipmentation-gata6-region1.png" /></p>
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<div class="small-12 columns">
<p><small><strong>Region 2</strong></small></p>
<center><img src="../../img/product/kits/chipmentation-gata6-region2.png" /></center></div>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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$featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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	'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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            <h6 style="height:60px">24 SI for ChIPmentation</h6>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<li>Flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows you to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>Tagmentationは、酵素（トランスポザーゼ）がDNAを切断し、DNAフラグメントの末端にシーケンスアダプターを1ステップで組み込む反応を言います。 DiagenodeのChIPmentationテクノロジーでは、クロマチン免疫沈降とタグ付けを1つの合理化されたワークフローで組み合わせ、Tagmentationがクロマチンで直接行われます。 TAG Kit for ChIPmentationは、独自の検証済みChIPプロトコールを使用してChIPmentation専用として開発されました。 TAG Kit for ChIPmentationには、Tagmentationベースのライブラリー調製用の試薬が含まれており、磁気ビーズに基づき、どんなChIPプロトコールとも互換性があります。 多重化のインデックスは個別に購入頂く必要があり、参照としてご利用頂けます。：24 SI for ChIPmentation、Cat。 番号C01011031</p>',
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<div align="center"><a href="https://www.diagenode.com/pages/form-chipmentation" class="center alert radius button"><i class="fa fa-info"></i> Contact us</a></div>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<li>flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation-2.jpg" /></center>
<p><strong></strong></p>
<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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<h2>ChIPmentation for high-quality and fast <br />ChIP-seq library preparation</h2>
<p>Diagenode&rsquo;s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.</p>
<p>Different protocols have been optimized bringing the solution for different needs:</p>
<ul class="no-bullet">
<li>✓ <strong>Low cell number</strong> &ndash; The kit <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">&micro;ChIPmentation for Histones</a> enables to use as little as 10.000 cells per reaction New!</li>
<li>✓ <strong>Standard cell number</strong> &ndash; <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation kit for Histones</a> - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.</li>
<li>✓<strong> Module for library prep using</strong> <strong>tagmentation</strong> &ndash; <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Tag Kit for ChIPmentation</a> can be used with any ChIP protocol</li>
</ul>
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<div class="extra-spaced">
<h2>Benefits of the ChIPmentation system for optimal ChIP-seq</h2>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> Ensure high quality sequencing data</li>
<li><i class="fa fa-arrow-circle-right"></i> Perform ChIP-seq on low input samples</li>
<li><i class="fa fa-arrow-circle-right"></i> Enjoy an easy and fast protocol</li>
<li><i class="fa fa-arrow-circle-right"></i> Benefit from the elimination of sequencing adaptor dimers</li>
<li><i class="fa fa-arrow-circle-right"></i> Automate on the IP-Star&reg; to support standardization and save time</li>
</ul>
</div>
<div class="extra-spaced">
<ul class="accordion" data-accordion="" style="margin-left: 0; padding-left: 0;">
<li class="accordion-navigation"><a href="#more" style="color: #13b29c; background-color: transparent; display: inline; padding: 0; margin-left: 0;">ChIPmentation workflow - Read more</a>
<div id="more" class="content"><center><img src="https://www.diagenode.com/img/product/kits/chipmentation-for-histones-workflow.png" alt="Diagenode-ChIPmentation" caption="false" width="728" height="1024" /></center>
<div class="spaced">In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star&reg;. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.</div>
</div>
</li>
</ul>
</div>
<div class="extra-spaced">
<h2>Complete kits:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v1" style="color: #13b29c;"><i class="fa fa-caret-right"></i> &micro;ChIPmentation for Histones</a>
<div id="v1" class="content">The Diagenode &micro;ChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.<br /><a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v2" style="color: #13b29c;"><i class="fa fa-caret-right"></i> ChIPmentation Kit for Histones </a>
<div id="v2" class="content">ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.<br /><a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">Read more</a></div>
</li>
</ul>
<h2>Modules:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v3" style="color: #13b29c;"><i class="fa fa-caret-right"></i> TAG Kit for ChIPmentation</a>
<div id="v3" class="content">The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.<br /><a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">Read more</a></div>
</li>
</ul>
<h2>Primer indexes for tagmented libraries:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v4" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for ChIPmentation</a>
<div id="v4" class="content">The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.<br /><a href="https://www.diagenode.com/en/p/24-si-for-chipmentation">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 SI for Tagmented libraries</a>
<div id="v5" class="content">The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&amp;Tag technologies.<br /><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v6" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set I</a>
<div id="v6" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v7" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set II</a>
<div id="v7" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries &ndash; Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.<br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v8" style="color: #13b29c;"><i class="fa fa-caret-right"></i> 24 UDI for Tagmented libraries - Set III</a>
<div id="v8" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">Read more</a></div>
</li>
<li class="accordion-navigation"><br />
<div id="v9" class="content">The 24 UDI (Unique dual indexes) for tagmented libraries &ndash; Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms. <br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set4">Read more</a></div>
</li>
</ul>
<h2>Separately available products:</h2>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v10" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - loaded</a>
<div id="v10" class="content">Diagenode Tagmentase &ndash; loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.<br /><a href="https://www.diagenode.com/en/p/tagmentase-loaded-30">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v11" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase (Tn5 transposase) - unloaded</a>
<div id="v11" class="content">Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.<br /><a href="https://www.diagenode.com/en/p/tagmentase">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v12" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentase Dilution Buffer</a>
<div id="v12" class="content">Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.<br /><a href="https://www.diagenode.com/en/p/tagmentase-dilution-buffer">Read more</a></div>
</li>
<li class="accordion-navigation"><a href="#v13" style="color: #13b29c;"><i class="fa fa-caret-right"></i> Tagmentation Buffer (1x)</a>
<div id="v13" class="content">Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.<br /><a href="https://www.diagenode.com/en/p/tagmentation-buffer-1x-1ml">Read more</a></div>
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<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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			'description' => '<p>One of our issues was that we could obtain only a limited number of cells, which is not enough for canonical ChIP-seq protocols. To solve this issue, we used the Diagenode ChIPmentation solution composed of iDeal ChIP-seq Kit for Transcription Factor, TAG Kit for ChIPmentation, and 24 SI for ChIPmentation. We performed ChIPmentation with IP-Star automated system for GATA6 in 2 million GATA6-overxpressing iPS cells. The result showed clear signal/noise ratio and was highly reproducible. This solution also worked in vitro differentiated definitive endoderm cells (data not shown).</p>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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<ul>
<li>smaller sample requirement,</li>
<li>simpler workflow with less that can go wrong,</li>
<li>slightly higher resolution and signal: noise ratio.</li>
</ul>
<div class="small-12 columns"><center><img src="../../img/product/kits/chipmentation-sequencing-p65.png" /></center></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>ChIPmentation sequencing profiles for p65. </strong>Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031). </small></p>
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</div><cite>Researcher from University of Nice-Sophia Antipolis, Nice, France</cite></blockquote>
'
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Region 1</strong></small></p>
<center>
<p><img src="../../img/product/kits/chipmentation-gata6-region1.png" /></p>
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<div class="small-12 columns">
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<div class="extra-spaced"></div>
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<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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</div><cite>Takahiro Suzuki, Ph.D., Senior Research Scientist, Cellular Function Conversion Technology Team, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan</cite></blockquote>
'
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<div class="small-12 columns">
<p><small><strong>Figure 1. ChIPmentation sequencing profiles for Gata6</strong><br />Chromatin preparation and immunoprecipitation have been performed on hiPSCs (human induced Pluripotent Stem Cells) overexpressing Gata6 using the <a href="../../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq kit for TFs</a> (Cat. No. C01010055). Chromatin from 2,000,000 cells was used for the immunoprecipitation in combination with either anti-GATA6 antibody. The library preparation was performed with the <a href="../../p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030) and <a href="../../p/24-si-for-chipmentation">24 SI for ChIPmentation</a> (Cat. No. C01011031).</small></p>
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>24 SI for ChIPmentation個カートに追加。</p>

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            <h6 style="height:60px">24 SI for ChIPmentation</h6>
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<p>The 24 SI for ChIPmentation includes 24 single primer indexes completing the <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a>. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.</p>
<p>The primer indexes of the 24 SI for ChIPmentation are also compatible with other <strong>tagmentation-based library preparation</strong> <strong>protocols</strong>, such as <strong>ATAC-seq</strong> or <strong>CUT&amp;Tag</strong> technologies.</p>',
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<li>flexibility &ndash; the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with <strong>any ChIP protocol </strong>(based on magnetic beads)<strong><br /></strong></li>
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<h3>ChIPmentation on histone marks using TAG KIT for ChIPmentation</h3>
<center><img src="https://www.diagenode.com/img/product/chipmentation/chipmentation-of-histone-using-tag-kit-for-chipmentation.jpg" /></center>
<p><strong>ChIPmentation sequencing results for four histone marks</strong>. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star&reg; Compact Automated System.</p>
<p><small>*The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).</small></p>
<h3>ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation</h3>
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<p><strong>ChIPmentation sequencing results for two non histone factors</strong>. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).</p>',
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TAG Kit for ChIPmentation MANUAL Diagenode’s TAG Kit for ChIPmentation in combination with the set of indexes (24 SI for ChI...	Download
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