Diagenode

Tagmentase (Tn5 transposase) – loaded EARLY ACCESS

Catalog Number
Format
Price
C01070012-30
30 μl
$330.00
Other format

Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters. Its ability to cut DNA and insert the sequencing adapters in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation.

Using Diagenode’s Tagmentase (Tn5 transposase) - loaded you may need also:

Looking for unloaded Tagmentase? Please go to Tagmentase (Tn5 transposase) – unloaded

TESTIMONIAL

We have been using the Hyperactive Tagmentase for 2 years and its performance is outstanding - short operation time and good reproducibility, outmatching the competition. Moreover the interaction with customer representatives is always top-notch - highly efficient and knowledgeable. I can't recommend enough!

Julia Liz Touza, AstraZeneca Gothenburg, Sweden
  • Examples of results

    Tagmentase (Tn5 transposase) – loaded can be used in any application using tagmentation-based library preparation, like for example ChIPmentation, ATAC-seq.  Here below we are showing some examples of results.

    Tn5 transposase Enxzymes

    Figure 1. Typical library profile of ATAC-seq library generated with the Tagmentase
    ATAC-seq has been performed on 50,000 nuclei from K562 cells using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). The library profile has been generated on a Fragment Analyzer (Agilent).

    Diagenode Tagmentation

    Figure 2. Enrichments at TSS of ATAC-seq libraries generated with the Tagmentase
    ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043). Heatmaps around the hg19 TSS were generated using deeptools plotHeatmap functionality.

    Transposase enzymes for ATAC-Seq

    Figure 3. Sequencing profiles of ATAC-seq libraries generated with the Tagmentase
    ATAC-seq has been performed on 50,000 nuclei from K562 cells, in triplicates, using the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) and Tagmentation Buffer (2x) (Cat. No. C01019043).

    purified Transposase enzymes

    Figure 4. Typical library profile of ChIPmentation library generated with the Tagmentase
    Chromatin preparation and immunoprecipitation have been performed on 1,000,000 K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Diagenode antibody targeting H3K4me3 (Cat. No. C15410003) and with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012). The library profile has been generated on a Fragment Analyzer (Agilent).

    pA-Tn5 Transposase Enzymes

    Figure 5. Sequencing profiles of ChIPmentation libraries generated with the Tagmentase
    Chromatin preparation and immunoprecipitation have been performed on 500.000 to 1,000,000 cells using the ChIPmentation Kit for Histones (Cat. No. C01011010) in combination with the Tagmentase (Tn5 transposase) – loaded (Cat. No. C01070012) on K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K36me3 (Cat. No. C15410192), H3K27me3 (Cat. No. C15410195) and rabbit IgG (Cat. No. C15410206) have been used.

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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Tagmentase (Tn5 transposase) – loaded EARLY ACCESS (Diagenode Cat# C01070012-30). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    High-throughput sequencing of insect specimens with sub-optimal DNA preservation using a practical, plate-based Illumina-compatible Tn5 transposase library preparation method
    Cobb L. et all.
    Entomological sampling and storage conditions often prioritise efficiency, practicality and conservation of morphological characteristics, and may therefore be suboptimal for DNA preservation. This practice can impact downstream molecular applications, such as the generation of high-throughput genomic libraries, whi...

    On the identification of differentially-active transcription factors from ATAC-seq data
    Gerbaldo F. et al.
    ATAC-seq has emerged as a rich epigenome profiling technique, and is commonly used to identify Transcription Factors (TFs) underlying given phenomena. A number of methods can be used to identify differentially-active TFs through the accessibility of their DNA-binding motif, however little is known on the best approa...

    Cellular reprogramming in vivo initiated by SOX4 pioneer factor activity
    Katsuda T.
    Tissue damage elicits cell fate switching through a process called metaplasia, but how the starting cell fate is silenced and the new cell fate is activated has not been investigated in animals. In cell culture, pioneer transcription factors mediate “reprogramming” by opening new chromatin sites for expr...

    The ncBAF complex regulates transcription in AML through H3K27ac sensing by BRD9
    Klein D.C. et al.
    The non-canonical BAF complex (ncBAF) subunit BRD9 is essential for acute myeloid leukemia (AML) cell viability but has an unclear role in leukemogenesis. Because BRD9 is required for ncBAF complex assembly through its DUF3512 domain, precise bromodomain inhibition is necessary to parse the role of BRD9 as a transcr...

    ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse
    Menon D.U. et al.
    We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I...

    Zfp296 knockout enhances chromatin accessibility and induces a uniquestate of pluripotency in embryonic stem cells.
    Miyazaki S. et al.
    The Zfp296 gene encodes a zinc finger-type protein. Its expression is high in mouse embryonic stem cells (ESCs) but rapidly decreases following differentiation. Zfp296-knockout (KO) ESCs grew as flat colonies, which were reverted to rounded colonies by exogenous expression of Zfp296. KO ESCs could not form teratomas...

    YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.
    Yamazaki M. et al.
    Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-m...

    Analyzing genomic and epigenetic profiles in single cells by hybridtransposase (scGET-seq).
    Cittaro D. et al.
    scGET-seq simultaneously profiles euchromatin and heterochromatin. scGET-seq exploits the concurrent action of transposase Tn5 and its hybrid form TnH, which targets H3K9me3 domains. Here we present a step-by-step protocol to profile single cells by scGET-seq using a 10× Chromium Controller. We describ...

    A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.
    Zou Han et al.
    How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly availab...

    Physiological reprogramming in vivo mediated by Sox4 pioneer factoractivity
    Katsuda T. et al.
    Tissue damage elicits cell fate switching through a process called metaplasia, but how the starting cell fate is silenced and the new cell fate is activated has not been investigated in animals. In cell culture, pioneer transcription factors mediate “reprogramming” by opening new chromatin sites for expr...

    EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.
    Zolotarev Nikolay et al.
    The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to naïve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whet...

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