Diagenode

Tagmentase (Tn5 transposase) - unloaded

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Catalog Number
Format
Price
C01070010-20
20 µl
$515.00
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Tagmentase (Tn5 transposase)

Diagenode Tagmentase is a hyperactive Tn5 transposase with the potential to enhance epigenetic studies. Its ability to cut DNA and insert sequences of interest in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq, ChIPmentation, CHANGE-seq and other. The enzyme is not loaded with DNA oligos, providing flexibility of application. To ensure optimal results the concentration may be adjusted with Diagenode Tagmentase Dilution Buffer (Cat. No. C01070011), available separately.

Protocol for transposome assembly

Using Diagenode’s Tagmentase (Tn5 transposase) you may also need:

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    Tn5 transposase

    Tagmentase Tn5 transposase

    Figure 1: Efficient fragmentation of the lambda DNA after incubation with the Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with diluted Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). Profiles show the size of lambda DNA before (A) and after treatment with Tagmentase (B).

    Tn5 transposase perfect for NGS

    Figure 2: Fragmentation efficiency depending on the amount of Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The Tagmentase was previously diluted with the Tagmentase Dilution Buffer (Cat. No.) at ¼ and 1/16 dilutions. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). The migration of the samples shows variations of the size distribution according to the amount of Tagmentase used for the reaction.

  •  Documents
    Tagmentase datasheet DATASHEET
    Diagenode Tagmentase is a hyperactive transposase with the ability to cut DNA and insert sequence...
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    Transposome assembly using Diagenode Tagmentase PROTOCOL
    Transposome assembly using Diagenode Tagmentase protocol
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  •  Safety sheets
    Tagmentase Tn5 transposase SDS US en Download
    Tagmentase Tn5 transposase SDS GB en Download
    Tagmentase Tn5 transposase SDS BE fr Download
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    Tagmentase Tn5 transposase SDS JP ja Download
    Tagmentase Tn5 transposase SDS BE nl Download
  •  Publications

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    Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
    Liguo Zhang et al.
    Prolonged detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and recurrence of PCR-positive tests have been widely reported in patients after recovery from COVID-19, but some of these patients do not appear to shed infectious virus. We investigated the possibility that SARS-CoV-2 RNAs can ...

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