Diagenode

Tagmentase (Tn5 transposase) - unloaded

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Catalog Number
Format
Price
C01070010-10
10 µl
$355.00
Other format

Tagmentase (Tn5 transposase)

Diagenode Tagmentase is a hyperactive Tn5 transposase with the potential to enhance epigenetic studies. Its ability to cut DNA and insert sequences of interest in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq, ChIPmentation, CHANGE-seq and other. The enzyme is not loaded with DNA oligos, providing flexibility of application. To ensure optimal results the concentration may be adjusted with Diagenode Tagmentase Dilution Buffer (Cat. No. C01070011), available separately.

Protocol for transposome assembly

Using Diagenode’s Tagmentase (Tn5 transposase) you may also need:

Looking for loaded Tagmentase? Please go to Tagmentase (Tn5 transposase) - loaded.

TESTIMONIAL

We experienced strong purity and activity differences between in-house produced Tn5 batches and switched to buying Tn5 from Diagenode with higher activity and small batch effects only.

Rebekka Scholz et al. Combined Analysis of mRNA Expression and Open Chromatin in Microglia. Methods Mol Biol. 2024;2713:543-571.
TESTIMONIAL

We have been using the Hyperactive Tagmentase for 2 years and its performance is outstanding - short operation time and good reproducibility, outmatching the competition. Moreover the interaction with customer representatives is always top-notch - highly efficient and knowledgeable. I can't recommend enough!

Julia Liz Touza, AstraZeneca Gothenburg, Sweden
  • Quality control

    Tn5 transposase

    Tagmentase Tn5 transposase

    Figure 1: Efficient fragmentation of the lambda DNA after incubation with the Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with diluted Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). Profiles show the size of lambda DNA before (A) and after treatment with Tagmentase (B).

    Tn5 transposase perfect for NGS

    Figure 2: Fragmentation efficiency depending on the amount of Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The Tagmentase was previously diluted with the Tagmentase Dilution Buffer (Cat. No.) at ¼ and 1/16 dilutions. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). The migration of the samples shows variations of the size distribution according to the amount of Tagmentase used for the reaction.

  •  Documents
    Tagmentase datasheet DATASHEET
    Diagenode Tagmentase is a hyperactive transposase with the ability to cut DNA and insert sequence...
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    Transposome assembly using Diagenode Tagmentase PROTOCOL
    Transposome assembly using Diagenode Tagmentase protocol
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  •  Safety sheets
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  •  Publications

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    CompDuplex: Accurate detection of somatic mutations by duplex-seq with comprehensive genome coverage
    Muchun Niu et al.
    Somatic mutations continuously accumulate in the human genome, posing vulnerabilities towards aging and increased risk of various diseases. However, accurate detection of somatic mutations at the whole genome scale is still challenging. By tagging and independently sequencing the two complementar...

    Integrative functional genomic analyses identify genetic variants influencing skin pigmentation in Africans
    Yuanqing Feng et al.
    Skin color is highly variable in Africans, yet little is known about the underlying molecular mechanism. Here we applied massively parallel reporter assays to screen 1,157 candidate variants influencing skin pigmentation in Africans and identified 165 single-nucleotide polymorphisms showing differential regulatory a...

    A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo
    Bestas B. et al.
    Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strategies for mapping off-target effects have emerged, but they suffer from limited sensitivity. To i...

    Combined Analysis of mRNA Expression and Open Chromatin in Microglia
    Scholz R.et al.
    The advance of single-cell RNA-sequencing technologies in the past years has enabled unprecedented insights into the complexity and heterogeneity of microglial cell states in the homeostatic and diseased brain. This includes rather complex proteomic, metabolomic, morphological, transcriptomic, and epigenetic adaptat...

    Volumetric imaging of an intact organism by a distributed molecular network
    Nianchao Qian and Joshua A Weinstein
    Lymphatic, nervous, and tumoral tissues, among others, exhibit physiology that emerges from three-dimensional interactions between genetically unique cells. A technology capable of volumetrically imaging transcriptomes, genotypes, and morphologies in a single de novo measurement would therefore provide a critical vi...

    Spatial epigenome-transcriptome co-profiling of mammalian tissues.
    Zhang D. et al.
    Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relatio...

    Analyzing genomic and epigenetic profiles in single cells by hybridtransposase (scGET-seq).
    Cittaro D. et al.
    scGET-seq simultaneously profiles euchromatin and heterochromatin. scGET-seq exploits the concurrent action of transposase Tn5 and its hybrid form TnH, which targets H3K9me3 domains. Here we present a step-by-step protocol to profile single cells by scGET-seq using a 10× Chromium Controller. We describ...

    Imaging Chromatin Accessibility by Assay ofTransposase-Accessible Chromatin with Visualization.
    Miyanari Yusuke
    Chromatin accessibility is one of the fundamental structures regulating genome functions including transcription and DNA repair. Recent technological advantages to analyze chromatin accessibility begun to explore the dynamics of local chromatin structures. Here I describe protocols for Assay of Transposase-Accessibl...

    Mouse kidney nuclear isolation and library preparation for single-cell combinatorial indexing RNA sequencing
    Li Haikuo and Humphreys Benjamin D.
    Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq3) enables high-throughput single-nucleus transcriptomic profiling of multiple samples in one experiment. Here, we describe an optimized protocol of mouse kidney nuclei isolation and sci-RNA-seq3 library preparation. The use of a dounce tissue homogenizer...

    Optimized single-nucleus transcriptional profiling by combinatorialindexing.
    Martin Beth K et al.
    Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for recovering gene expression data from an exponentially scalable number of individual cells or nuclei. However, sci-RNA-seq is a complex protocol that has historically exhibited variable performance on different tissues, as well a...

    Spatial profiling of chromatin accessibility in mouse and human tissues
    Yanxiang Deng et al.
    Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context1. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping2,3,4,5, but the ability to capture spatial epigenetic informati...

    Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level
    Fan Rong et al.
    Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship ac...

    Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
    Liguo Zhang et al.
    Prolonged detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and recurrence of PCR-positive tests have been widely reported in patients after recovery from COVID-19, but some of these patients do not appear to shed infectious virus. We investigated the possibility that SARS-CoV-2 RNAs can ...

    T-RHEX-RNAseq – A tagmentation-based, rRNA blocked, randomhexamer primed RNAseq method for generating stranded RNAseq librariesdirectly from very low numbers of lysed cells
    Gustafsson Charlotte et al.
    Background: RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging. Results: We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNA...

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