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Tagmentase (Tn5 transposase)

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Catalog Number
Format
Price
C01070010-10
10 µl
$290.00
Other format

Tagmentase

Diagenode Tagmentase is a hyperactive Tn5 transposase with the potential to enhance epigenetic studies. Its ability to cut DNA and insert sequences of interest in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation. The enzyme is not loaded with DNA oligos, providing flexibility of application. To ensure optimal results the concentration may be adjusted with Diagenode Tagmentase Dilution Buffer (Cat. No. C01070010), available separately.

Protocol for transposome assembly

  • Quality control

    Figure 1: Efficient fragmentation of the lambda DNA after incubation with the Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with diluted Diagenode Tagmentase (Cat. No. C01070010) for 7 min at 55°C. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on the Diagenode IP-Star Compact Automated System, the size of the DNA was assessed on the Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). Profiles show the size of lambda DNA before (A) and after treatment with Tagmentase (B).

    Figure 2: Fragmentation efficiency depending on the amount of Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with Diagenode Tagmentase (Cat. No. C01070010) for 7 min at 55°C. The Tagmentase was previously diluted with the Tagmentase Dilution Buffer (Cat. No. C01070011) at ¼ and 1/16 dilutions. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on the Diagenode IP-Star Compact Automated System, the size of the DNA was assessed on the Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). The migration of the samples shows variations of the size distribution according to the amount of Tagmentase used for the reaction.

  •  Documents
    Tagmentase datasheet DATASHEET
    Diagenode Tagmentase is a hyperactive transposase with the ability to cut DNA and insert sequence...
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    Transposome assembly using Diagenode Tagmentase PROTOCOL
    Transposome assembly using Diagenode Tagmentase protocol
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  •  Safety sheets
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    Tagmentase Tn5 transposase SDS BE fr Download
    Tagmentase Tn5 transposase SDS ES es Download
  •  Publications

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