Tagmentation Buffer (2x)

Catalog Number
300 µl
Other format

Diagenode Tagmentation Buffer (2x) is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode Tagmentase (Tn5 transposase) on DNA or chromatin samples, as half of the total volume reaction like in ATAC-seq protocol.

  • Examples of use

    ATAC-seq experiments:

    • After cell lysis and nuclei isolation, the nuclei pellets can be incubated with the following mix for 1 reaction:
    Tagmentation Buffer (2x) 25 µl
    Tagmentase loaded 2.5 µl
    Digitonin 1% 0.5 µl
    Tween20 10% 0.5 µl
    PBS 16.5 µl
    Nuclease-free water  5 µl
    Nuclei pellet*

    *  The number of nuclei per reaction will depend on the ATAC-seq experimental design. Successful tagmentation with the proposed protocol has been performed on 50,000 nuclei per reaction. 

    • The reaction is then incubated 30 minutes at 37°C.
    • The tagmentation reaction can then be stopped by addition of 250 µl of DNA Binding buffer from Diagenode MicroChIP DiaPure Columns (Cat. No. C03040001).
    • The tagmented libraries can then be purified using the MicroChIP DiaPure Columns (Cat. No. C03040001), and amplified.
  •  Documents
  •  Safety sheets
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  •  Publications

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    ARID1A governs the silencing of sex-linked transcription during male meiosis in the mouse
    Menon D.U. et al.
    We present evidence implicating the BAF (BRG1/BRM Associated Factor) chromatin remodeler in meiotic sex chromosome inactivation (MSCI). By immunofluorescence (IF), the putative BAF DNA binding subunit, ARID1A (AT-rich Interaction Domain 1a), appeared enriched on the male sex chromosomes during diplonema of meiosis I...

    YAP/BRD4-controlled ROR1 promotes tumor-initiating cells andhyperproliferation in pancreatic cancer.
    Yamazaki M. et al.
    Tumor-initiating cells are major drivers of chemoresistance and attractive targets for cancer therapy, however, their identity in human pancreatic ductal adenocarcinoma (PDAC) and the key molecules underlying their traits remain poorly understood. Here, we show that a cellular subpopulation with partial epithelial-m...

    A neurodevelopmental epigenetic programme mediated bySMARCD3-DAB1-Reelin signalling is hijacked to promote medulloblastomametastasis.
    Zou Han et al.
    How abnormal neurodevelopment relates to the tumour aggressiveness of medulloblastoma (MB), the most common type of embryonal tumour, remains elusive. Here we uncover a neurodevelopmental epigenomic programme that is hijacked to induce MB metastatic dissemination. Unsupervised analyses of integrated publicly availab...

    EBF1 is continuously required for stabilizing local chromatinaccessibility in pro-B cells.
    Zolotarev Nikolay et al.
    The establishment of de novo chromatin accessibility in lymphoid progenitors requires the "pioneering" function of transcription factor (TF) early B cell factor 1 (EBF1), which binds to naïve chromatin and induces accessibility by recruiting the BRG1 chromatin remodeler subunit. However, it remains unclear whet...


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