Diagenode

Tagmentase

default alt
目录号
格式
价格
C01070010-10
10 µl
$305.00
其他格式

Diagenode Tagmentase is a hyperactive Tn5 transposase with the potential to enhance epigenetic studies. Its ability to cut DNA and insert sequences of interest in one step makes it the perfect companion for Next-Generation Sequencing experiments using powerful technologies such as ATAC-seq or ChIPmentation. The enzyme is not loaded with DNA oligos, providing flexibility of application. To ensure optimal results the concentration may be adjusted with Diagenode Tagmentase Dilution Buffer (Cat. No. C01070010), available separately.

Contact us if you need a protocol for transposome assembly

  • Quality control

    Tn5 transposase

    Tagmentase Tn5 transposase

    Figure 1: Efficient fragmentation of the lambda DNA after incubation with the Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with diluted Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). Profiles show the size of lambda DNA before (A) and after treatment with Tagmentase (B).

    Tn5 transposase perfect for NGS

    Figure 2: Fragmentation efficiency depending on the amount of Tagmentase
    For fragmentation, 100 ng of DNA from bacteriophage lambda were incubated with Diagenode Tagmentase (Cat. No. C01070010) and Tagmentation buffer (1x) (Cat. No. C01019042) for 7 min at 55°C. The Tagmentase was previously diluted with the Tagmentase Dilution Buffer (Cat. No.) at ¼ and 1/16 dilutions. The reaction was stopped by addition of SDS (0.2% final concentration). After clean-up using AMPure XP beads (Beckman Coulter) on Diagenode IP-Star robot, the size of the DNA was assessed on Fragment Analyzer (Agilent), using the HS Large Fragment 50kb Kit (Agilent). The migration of the samples shows variations of the size distribution according to the amount of Tagmentase used for the reaction.

  •  文档
    Tagmentase datasheet DATASHEET
    Diagenode Tagmentase is a hyperactive transposase with the ability to cut DNA and insert sequence...
    Download
    Transposome assembly using Diagenode Tagmentase PROTOCOL
    Transposome assembly using Diagenode Tagmentase protocol
    Download
  •  Safety sheets
    Tagmentase Tn5 transposase SDS GB en Download
    Tagmentase Tn5 transposase SDS US en Download
    Tagmentase Tn5 transposase SDS BE nl Download
    Tagmentase Tn5 transposase SDS BE fr Download
    Tagmentase Tn5 transposase SDS FR fr Download
    Tagmentase Tn5 transposase SDS ES es Download
    Tagmentase Tn5 transposase SDS DE de Download
    Tagmentase Tn5 transposase SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Tagmentase (Diagenode Cat# C01070010-10). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Imaging Chromatin Accessibility by Assay ofTransposase-Accessible Chromatin with Visualization.
    Miyanari Yusuke
    Chromatin accessibility is one of the fundamental structures regulating genome functions including transcription and DNA repair. Recent technological advantages to analyze chromatin accessibility begun to explore the dynamics of local chromatin structures. Here I describe protocols for Assay of Transposase-Accessibl...

    Optimized single-nucleus transcriptional profiling by combinatorialindexing.
    Martin Beth K et al.
    Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for recovering gene expression data from an exponentially scalable number of individual cells or nuclei. However, sci-RNA-seq is a complex protocol that has historically exhibited variable performance on different tissues, as well a...

    Spatial profiling of chromatin accessibility in mouse and human tissues
    Yanxiang Deng et al.
    Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context1. The emergence of spatial transcriptomics has enabled genome-scale gene expression mapping2,3,4,5, but the ability to capture spatial epigenetic informati...

    Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level
    Fan Rong et al.
    Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship ac...

    Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
    Liguo Zhang et al.
    Prolonged detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and recurrence of PCR-positive tests have been widely reported in patients after recovery from COVID-19, but some of these patients do not appear to shed infectious virus. We investigated the possibility that SARS-CoV-2 RNAs can ...

  •  相关产品

活动

  • 64th ASH Annual Meeting and Exposition 2022
    New Orleans, Louisiana, USA
    Dec 10-Dec 13, 2022
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics