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µChIPmentation for Histones

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カタログ番号
フォーマット
価格
C01011011
24 rxns
$2,193.00
旧フォーマット

Diagenodeの µChIPmentation Kit for Histonesは、わずか10.000個の細胞で細胞固定から精製ライブラリーに渡るChIP-seqが可能です。チューブからチューブへのサンプル転送数を制限し、DNAの損失を減少します。ワークフロー全体(細胞固定からライブラリー精製まで)で1サンプル当たり使用するチューブ数はわずか3本で、ステップ数、サンプル転送数の削減、およびChIPmentation technologyテクノロジー自体により、限られた量のサンプルで効率的かつ堅牢なChIP-seqが実現します。このキットµChIPmentation for Histonesには、クロマチン調製、クロマチン免疫沈降、およびタグ付けを使用したライブラリー調製用に必要なすべての試薬が含まれています。マルチプレックス用のプライマーインデックスはキットに含まれていませんので、別途購入していただく必要があります。

  • 特徴

    ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.

    A.
    B.

    Figure 1. Comparison between ChIPmentation and µChIPmentation
    ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.
    A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).

    Figure 2. Sequencing profiles of µChIPmentation libraries
    Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.

    Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No.  C15410195) and 10.000 cells of K562 cells per immunoprecipitation.
    Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.

  • ヒストン用µChIPmentationキットの追加ソリューション

    Chromatin EasyShear Kit - High SDS optimizes chromatin shearing, a critical step for ChIP.

    ChIP-seq grade anti-histone antibodies provide high yields with excellent specificity and sensitivity.

    For fast and efficient isolation of magnetic beads we recommend the magnetic racks DiaMag1.5 and DiaMag0.2.

    Primer indexes for multiplexing:

  •  資料
    µChIPmentation Kit for Histones MANUAL
    Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) is the method of ...
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  •  Safety sheets
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  •  出版物

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