Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'product' => array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
)
$language = 'en'
$meta_keywords = ''
$meta_description = ''
$meta_title = '24 unique dual indexes for tagmented libraries'
$product = array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '332',
'name' => 'UDI for Tagmented librairies',
'product_id' => '3158',
'modified' => '2020-12-09 15:39:40',
'created' => '2020-12-09 15:39:40'
),
'Master' => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '3083',
'antibody_id' => null,
'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
'label3' => '',
'info3' => '',
'format' => '24 rxns',
'catalog_number' => 'C01011011',
'old_catalog_number' => '',
'sf_code' => 'C01011011-',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '1950',
'price_USD' => '2280',
'price_GBP' => '1780',
'price_JPY' => '339600',
'price_CNY' => '/',
'price_AUD' => '5700',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'uchipmentation-for-histones-24-rxns',
'meta_title' => 'µChIPmentation for Histones 24 rxns',
'meta_keywords' => '',
'meta_description' => 'µChIPmentation for Histones 24 rxns',
'modified' => '2023-04-20 16:03:27',
'created' => '2020-06-24 09:54:27',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '3',
'position' => '4',
'parent_id' => null,
'name' => 'Kits',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'kits',
'cookies_tag_id' => null,
'meta_keywords' => 'DNA methylation Kits,ChIP and ChIP-seq kits,Library preparation kits,MicroChIP/MicroPlex Kit,iDeal Library Preparation Kit',
'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA Immunoprecipitation',
'meta_title' => 'Isotype controls for Methylated DNA Immunoprecipitation | Diagenode',
'modified' => '2019-07-03 10:48:00',
'created' => '2014-11-13 22:28:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '152',
'position' => '4',
'parent_id' => '5',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
</ul>
<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'primer-indexes-for-tagmented-libraries',
'cookies_tag_id' => null,
'meta_keywords' => 'Primer indexes for tagmented libraries category',
'meta_description' => 'Primer indexes for tagmented libraries category',
'meta_title' => 'Primer indexes for tagmented libraries category',
'modified' => '2022-03-25 11:22:03',
'created' => '2022-03-24 10:26:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2136',
'name' => '8 UDI for tagmented libraries SDS US en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2134',
'name' => '8 UDI for tagmented libraries SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2129',
'name' => '8 UDI for tagmented libraries SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2133',
'name' => '8 UDI for tagmented libraries SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2132',
'name' => '8 UDI for tagmented libraries SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2131',
'name' => '8 UDI for tagmented libraries SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2135',
'name' => '8 UDI for tagmented libraries SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
)
$pro = array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'ProductsGroup' => array(
'id' => '373',
'product_id' => '3159',
'group_id' => '332'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/uchipmentation-for-histones-24-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011011</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3083" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3083" id="CartAdd/3083Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3083" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="uchipmentation-for-histones-24-rxns" data-reveal-id="cartModal-3083" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">µChIPmentation Kit for Histones</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011034</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3158" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3158" id="CartAdd/3158Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3158" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 24 UDI for Tagmented libraries - Set I</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="24-unique-dual-indexes-for-tagmented-libraries-set1" data-reveal-id="cartModal-3158" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">24 UDI for Tagmented libraries - Set I</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
'id' => '4677',
'product_id' => '3159',
'related_id' => '3158'
),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
'id' => '3042',
'product_id' => '3159',
'document_id' => '1110'
)
)
$sds = array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
'id' => '3689',
'product_id' => '3159',
'safety_sheet_id' => '2130'
)
)
$publication = array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
'id' => '6687',
'product_id' => '3159',
'publication_id' => '4599'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36711883" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'product' => array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
)
$language = 'en'
$meta_keywords = ''
$meta_description = ''
$meta_title = '24 unique dual indexes for tagmented libraries'
$product = array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '332',
'name' => 'UDI for Tagmented librairies',
'product_id' => '3158',
'modified' => '2020-12-09 15:39:40',
'created' => '2020-12-09 15:39:40'
),
'Master' => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '3083',
'antibody_id' => null,
'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
'label3' => '',
'info3' => '',
'format' => '24 rxns',
'catalog_number' => 'C01011011',
'old_catalog_number' => '',
'sf_code' => 'C01011011-',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '1950',
'price_USD' => '2280',
'price_GBP' => '1780',
'price_JPY' => '339600',
'price_CNY' => '/',
'price_AUD' => '5700',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'uchipmentation-for-histones-24-rxns',
'meta_title' => 'µChIPmentation for Histones 24 rxns',
'meta_keywords' => '',
'meta_description' => 'µChIPmentation for Histones 24 rxns',
'modified' => '2023-04-20 16:03:27',
'created' => '2020-06-24 09:54:27',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '3',
'position' => '4',
'parent_id' => null,
'name' => 'Kits',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'kits',
'cookies_tag_id' => null,
'meta_keywords' => 'DNA methylation Kits,ChIP and ChIP-seq kits,Library preparation kits,MicroChIP/MicroPlex Kit,iDeal Library Preparation Kit',
'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA Immunoprecipitation',
'meta_title' => 'Isotype controls for Methylated DNA Immunoprecipitation | Diagenode',
'modified' => '2019-07-03 10:48:00',
'created' => '2014-11-13 22:28:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '152',
'position' => '4',
'parent_id' => '5',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
</ul>
<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'primer-indexes-for-tagmented-libraries',
'cookies_tag_id' => null,
'meta_keywords' => 'Primer indexes for tagmented libraries category',
'meta_description' => 'Primer indexes for tagmented libraries category',
'meta_title' => 'Primer indexes for tagmented libraries category',
'modified' => '2022-03-25 11:22:03',
'created' => '2022-03-24 10:26:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2136',
'name' => '8 UDI for tagmented libraries SDS US en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2134',
'name' => '8 UDI for tagmented libraries SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2129',
'name' => '8 UDI for tagmented libraries SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2133',
'name' => '8 UDI for tagmented libraries SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2132',
'name' => '8 UDI for tagmented libraries SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2131',
'name' => '8 UDI for tagmented libraries SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2135',
'name' => '8 UDI for tagmented libraries SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
)
$pro = array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'ProductsGroup' => array(
'id' => '373',
'product_id' => '3159',
'group_id' => '332'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/uchipmentation-for-histones-24-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011011</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3083" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3083" id="CartAdd/3083Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3083" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="uchipmentation-for-histones-24-rxns" data-reveal-id="cartModal-3083" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">µChIPmentation Kit for Histones</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011034</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3158" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3158" id="CartAdd/3158Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3158" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 24 UDI for Tagmented libraries - Set I</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="24-unique-dual-indexes-for-tagmented-libraries-set1" data-reveal-id="cartModal-3158" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">24 UDI for Tagmented libraries - Set I</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
'id' => '4677',
'product_id' => '3159',
'related_id' => '3158'
),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
'id' => '3042',
'product_id' => '3159',
'document_id' => '1110'
)
)
$sds = array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
'id' => '3689',
'product_id' => '3159',
'safety_sheet_id' => '2130'
)
)
$publication = array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
'id' => '6687',
'product_id' => '3159',
'publication_id' => '4599'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36711883" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'product' => array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
)
$language = 'en'
$meta_keywords = ''
$meta_description = ''
$meta_title = '24 unique dual indexes for tagmented libraries'
$product = array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '332',
'name' => 'UDI for Tagmented librairies',
'product_id' => '3158',
'modified' => '2020-12-09 15:39:40',
'created' => '2020-12-09 15:39:40'
),
'Master' => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '3083',
'antibody_id' => null,
'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
'label3' => '',
'info3' => '',
'format' => '24 rxns',
'catalog_number' => 'C01011011',
'old_catalog_number' => '',
'sf_code' => 'C01011011-',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '1950',
'price_USD' => '2280',
'price_GBP' => '1780',
'price_JPY' => '339600',
'price_CNY' => '/',
'price_AUD' => '5700',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'uchipmentation-for-histones-24-rxns',
'meta_title' => 'µChIPmentation for Histones 24 rxns',
'meta_keywords' => '',
'meta_description' => 'µChIPmentation for Histones 24 rxns',
'modified' => '2023-04-20 16:03:27',
'created' => '2020-06-24 09:54:27',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '3',
'position' => '4',
'parent_id' => null,
'name' => 'Kits',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'kits',
'cookies_tag_id' => null,
'meta_keywords' => 'DNA methylation Kits,ChIP and ChIP-seq kits,Library preparation kits,MicroChIP/MicroPlex Kit,iDeal Library Preparation Kit',
'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA Immunoprecipitation',
'meta_title' => 'Isotype controls for Methylated DNA Immunoprecipitation | Diagenode',
'modified' => '2019-07-03 10:48:00',
'created' => '2014-11-13 22:28:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '152',
'position' => '4',
'parent_id' => '5',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
</ul>
<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'primer-indexes-for-tagmented-libraries',
'cookies_tag_id' => null,
'meta_keywords' => 'Primer indexes for tagmented libraries category',
'meta_description' => 'Primer indexes for tagmented libraries category',
'meta_title' => 'Primer indexes for tagmented libraries category',
'modified' => '2022-03-25 11:22:03',
'created' => '2022-03-24 10:26:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2136',
'name' => '8 UDI for tagmented libraries SDS US en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2134',
'name' => '8 UDI for tagmented libraries SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2129',
'name' => '8 UDI for tagmented libraries SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2133',
'name' => '8 UDI for tagmented libraries SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2132',
'name' => '8 UDI for tagmented libraries SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2131',
'name' => '8 UDI for tagmented libraries SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2135',
'name' => '8 UDI for tagmented libraries SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
)
$pro = array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'ProductsGroup' => array(
'id' => '373',
'product_id' => '3159',
'group_id' => '332'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/uchipmentation-for-histones-24-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011011</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3083" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3083" id="CartAdd/3083Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3083" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="uchipmentation-for-histones-24-rxns" data-reveal-id="cartModal-3083" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">µChIPmentation Kit for Histones</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011034</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3158" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3158" id="CartAdd/3158Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3158" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 24 UDI for Tagmented libraries - Set I</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="24-unique-dual-indexes-for-tagmented-libraries-set1" data-reveal-id="cartModal-3158" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">24 UDI for Tagmented libraries - Set I</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
'id' => '4677',
'product_id' => '3159',
'related_id' => '3158'
),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
'id' => '3042',
'product_id' => '3159',
'document_id' => '1110'
)
)
$sds = array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
'id' => '3689',
'product_id' => '3159',
'safety_sheet_id' => '2130'
)
)
$publication = array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
'id' => '6687',
'product_id' => '3159',
'publication_id' => '4599'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36711883" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'en',
'meta_keywords' => '',
'meta_description' => '',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'product' => array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
)
$language = 'en'
$meta_keywords = ''
$meta_description = ''
$meta_title = '24 unique dual indexes for tagmented libraries'
$product = array(
'Product' => array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'locale' => 'eng'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '332',
'name' => 'UDI for Tagmented librairies',
'product_id' => '3158',
'modified' => '2020-12-09 15:39:40',
'created' => '2020-12-09 15:39:40'
),
'Master' => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(
(int) 0 => array(
'id' => '3083',
'antibody_id' => null,
'name' => 'µChIPmentation Kit for Histones',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/microchipmentation-for-histones.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The Diagenode <strong>µChIPmentation Kit for Histones</strong> is optimized to perform ChIP-seq on as little as <strong>10.000 cells</strong> from cell fixation to purified libraries. To <strong>reduce DNA lost</strong> the number of sample transfer from tube to tube has been limited: during whole workflow - from cell fixation to library prurification - only 3 tubes per sample are used. Reduced number of steps, reduced number of sample transfer and <a href="https://www.diagenode.com/en/categories/chromatin-ip-chipmentation" target="_blank">ChIPmentation technology</a> itself enable for <strong>efficient and robust ChIP-seq</strong> on <strong>limited amount of sample</strong>. The kit µChIPmentation for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation. The primer indexes for multiplexing are not included in the kit and have to be purchase separately - <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">read more</a>.</p>
<div class="extra-spaced">
<h3>Benefits of the µChIPmentation system for histone ChIP-seq</h3>
<ul>
<li><strong>Easier</strong> and <strong>faster</strong> than classical ChIP-seq</li>
<li>Optimized for <strong>low input samples</strong>: as little as 10,000 cells</li>
<li>Protocol optimized for <strong>FACS-sorted cells</strong></li>
<li>Validated for various <strong>histone marks</strong></li>
<li><strong>High quality</strong> sequencing data</li>
</ul>
</div>',
'label1' => 'Characteristics',
'info1' => '<p>ChIPmentation is based on tagmentation that allows library preparation to be integrated during the ChIP itself using transposase and sequencing-compatible adaptors. Our new improved µChIPmentation protocol combines 3 features for guaranteeing high quality sequencing data on small sample inputs 1) optimized chromatin shearing preparation protocol, 2) reduced number of steps, 3) reduced number of sample transfer from tube to tube - only 3 tubes per sample for the whole process, from cell fixation to purified libraries.</p>
<ul class="accordion" data-accordion="" style="margin-left: 0;">
<li class="accordion-navigation"><a style="background-color: white;" href="#workflow"><i class="fa fa-caret-right"></i> Workflow of µChIPmentation for Histones - Read more</a>
<div id="workflow" class="content">
<div class="extra-spaced"><center><img src="https://www.diagenode.com/img/product/kits/workflow-micro-chipmentation.png" /></center></div>
</div>
</li>
</ul>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns"><strong>A.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1A.png" /></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-5 large-5 columns" columns=""><strong>B.</strong><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig1B.png" /></div>
<div class="small-12 medium-7 large-7 columns">
<p style="text-align: left;"><strong>Figure 1. Comparison between ChIPmentation and µChIPmentation </strong><br />ChIPmentation: chromatin preparation has been performed on 7 M K562 cells using the ChIPmentation Kit for Histones (Cat. No. C01011000) and diluted chromatin from 500.000 cells was used for the immunoprecipitation. µChIPmentation: chromatin preparation and immunoprecipitation have been performed on 10.000 K562 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032). The Diagenode antibody targeting H3K27me3 (Cat. No. C15410195) was used.<br /> A. Distribution of the ChIPmentation and µChIPmentation readsets in a representative region of the genome (in duplicates). B. Comparison of the top 40% peaks from µChIPmentation (10.000 cells) with dataset generated with ChIPmentation (500.000 cells).</p>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig2.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 2. Sequencing profiles of µChIPmentation libraries </strong><br />Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 SI for Tagmented libraries (Cat. No. C010111032) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003), H3K27ac (Cat. No. C15410196), H3K27me3 (Cat. No. C15410195) and H3K9me3 (Cat. No. C15410193) have been used.</p>
</div>
</div>
</div>
<div class="extra-spaced">
<div class="row">
<div class="small-12 medium-12 large-12 columns"><img src="https://www.diagenode.com/img/product/kits/micro-chipmentation-fig3.png" /></div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 3. Integrative genomics viewer (IGV) visualization of from ChIP-seq experiments using H3K27me3 antibody (Diagenode, Cat. No. <span>C15410195</span>) and 10.000 cells of K562 cells per immunoprecipitation.<br /></strong>Cells were FACS-sorted and ChIP has been performed accordingly to µChIPmentation protocol. Batch chromatin preparation followed by immunoprecipitation has been used per comparison as indicated.</p>
</div>
</div>
</div>',
'label2' => 'Additional solutions for µChIPmentation Kit for Histones',
'info2' => '<p><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit - High SDS</a> optimizes chromatin shearing, a critical step for ChIP.</p>
<p><a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade anti-histone antibodies</a> provide high yields with excellent specificity and sensitivity.</p>
<p>For fast and efficient isolation of magnetic beads we recommend the magnetic racks <a href="https://www.diagenode.com/en/p/diamag02-magnetic-rack-1-unit">DiaMag0.2</a>.</p>
<p>Primer indexes for multiplexing:</p>
<ul class="no-bullet">
<li><a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries" target="_blank">24 SI for Tagmented libraries Cat. No. C01011032</a></li>
<li><a href="https://www.diagenode.com/en/p/8-si-for-tagmented-libraries" target="_blank">8 SI for Tagmented libraries Cat. No. C01011033</a></li>
<li><em></em><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">24 UDI for Tagmented libraries - Set I, Cat. No. C0101134</a></li>
<li><a href="https://www.diagenode.com/en/p/8-unique-dual-indexes-for-tagmented-libraries">8 UDI for Tagmented libraries, Cat. No. C0101135</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for Tagmented libraries - Set II, Cat. No. C0101136</a></li>
<li><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3">24 UDI for Tagmented libraries - Set III, Cat. No. C0101137</a></li>
<li></li>
</ul>',
'label3' => '',
'info3' => '',
'format' => '24 rxns',
'catalog_number' => 'C01011011',
'old_catalog_number' => '',
'sf_code' => 'C01011011-',
'type' => 'RFR',
'search_order' => '',
'price_EUR' => '1950',
'price_USD' => '2280',
'price_GBP' => '1780',
'price_JPY' => '339600',
'price_CNY' => '/',
'price_AUD' => '5700',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => true,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'uchipmentation-for-histones-24-rxns',
'meta_title' => 'µChIPmentation for Histones 24 rxns',
'meta_keywords' => '',
'meta_description' => 'µChIPmentation for Histones 24 rxns',
'modified' => '2023-04-20 16:03:27',
'created' => '2020-06-24 09:54:27',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
[maximum depth reached]
)
)
),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '3',
'position' => '4',
'parent_id' => null,
'name' => 'Kits',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p style="text-align: justify;">Diagenode’s epigenetic reagents include:</p>
<ul>
<li style="text-align: justify;"><strong>DNA methylation kits and antibodies</strong> - Validated NGS-compatible kits for MeDIP, MBD pull-down, whole genome bisulfite sequencing, and reduced representation bisulfite sequencing. Official provider for the original clone for 5-mC 33D3.</li>
<li style="text-align: justify;"><strong>ChIP and ChIP-seq kits for industry-leading specificity and sensitivity</strong> - MicroChIP/MicroPlex Kit for ChIP-seq with only 10,000 cells and the iDeal ChIP-seq Kits optimized for both transcription factors and histones. Our kits feature full reagents for ChIP-seq including control primers, control antibodies, magnetics beads, and purification reagents.</li>
<li style="text-align: justify;"><strong>Library preparation kits</strong> tailored for your specific requirements. The MicroPlex Library Preparation Kit simplifies library preparation requiring only 3 simple steps and allowing inputs of only 50 pg. </li>
</ul>
</div>
</div>',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'kits',
'cookies_tag_id' => null,
'meta_keywords' => 'DNA methylation Kits,ChIP and ChIP-seq kits,Library preparation kits,MicroChIP/MicroPlex Kit,iDeal Library Preparation Kit',
'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA Immunoprecipitation',
'meta_title' => 'Isotype controls for Methylated DNA Immunoprecipitation | Diagenode',
'modified' => '2019-07-03 10:48:00',
'created' => '2014-11-13 22:28:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
),
(int) 1 => array(
'id' => '152',
'position' => '4',
'parent_id' => '5',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p>The <strong>Primer indexes for tagmented libraries</strong> are PCR primers targeting the Nextera sequencing adapters, previously incorporated in the libraries by <strong>tagmentation</strong>. Each primer index is baring a <strong>unique index</strong> in order to identify each library before pooling them for sequencing in the same lane. They are compatible with any Nextera-based libraries such as the one generated with ChIPmentation, ATAC-seq or CUT&Tag technologies.</p>
<h3>Features:</h3>
<ul>
<li>Compatibility: <strong>tagmentation-based library</strong> preparation protocols</li>
<li>Flexibility: <strong>Single</strong> and <strong>unique dual indexing</strong> available</li>
<li>Multiplexing capacity: up to <strong>72 samples</strong> (with UDI)</li>
<li>Identification and <strong>filtering of index hopping</strong> – using the UDI</li>
</ul>
<p>Get the manual of <a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf" target="_blank" title="Primer indexes for tagmented libraries - Manual">Primer indexes for tagmented libraries</a>.</p>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => true,
'all_format' => false,
'is_antibody' => false,
'slug' => 'primer-indexes-for-tagmented-libraries',
'cookies_tag_id' => null,
'meta_keywords' => 'Primer indexes for tagmented libraries category',
'meta_description' => 'Primer indexes for tagmented libraries category',
'meta_title' => 'Primer indexes for tagmented libraries category',
'modified' => '2022-03-25 11:22:03',
'created' => '2022-03-24 10:26:41',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '2136',
'name' => '8 UDI for tagmented libraries SDS US en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '2134',
'name' => '8 UDI for tagmented libraries SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '2129',
'name' => '8 UDI for tagmented libraries SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '2133',
'name' => '8 UDI for tagmented libraries SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '2132',
'name' => '8 UDI for tagmented libraries SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '2131',
'name' => '8 UDI for tagmented libraries SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '2135',
'name' => '8 UDI for tagmented libraries SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
)
$pro = array(
'id' => '3159',
'antibody_id' => null,
'name' => '8 UDI for tagmented libraries',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <b>8</b><b> UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p>The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8<b> samples </b>for sequencing on Illumina platforms. This set of indexes is designed to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones (Cat. No. C01011011)</a>, <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones (Cat. No. C01011009)</a>, <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation (Cat. No. C01011030)</a>, <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit (Cat. No. C01080002)</a> but it is also compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <b>ATAC-seq</b> or <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '8 UDI',
'catalog_number' => 'C01011035',
'old_catalog_number' => '',
'sf_code' => 'C01011035-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '65',
'price_USD' => '70',
'price_GBP' => '60',
'price_JPY' => '11900',
'price_CNY' => '',
'price_AUD' => '175',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '8-unique-dual-indexes-for-tagmented-libraries',
'meta_title' => '24 unique dual indexes for tagmented libraries',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:00:04',
'created' => '2020-12-08 15:34:40',
'ProductsGroup' => array(
'id' => '373',
'product_id' => '3159',
'group_id' => '332'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/uchipmentation-for-histones-24-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011011</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3083" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3083" id="CartAdd/3083Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3083" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> µChIPmentation Kit for Histones</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('µChIPmentation Kit for Histones',
'C01011011',
'2280',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="uchipmentation-for-histones-24-rxns" data-reveal-id="cartModal-3083" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">µChIPmentation Kit for Histones</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01011034</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3158" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/3158" id="CartAdd/3158Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3158" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 24 UDI for Tagmented libraries - Set I</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('24 UDI for Tagmented libraries - Set I',
'C01011034',
'185',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="24-unique-dual-indexes-for-tagmented-libraries-set1" data-reveal-id="cartModal-3158" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">24 UDI for Tagmented libraries - Set I</h6>
</div>
</div>
</li>
'
$related = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23',
'ProductsRelated' => array(
'id' => '4677',
'product_id' => '3159',
'related_id' => '3158'
),
'Image' => array(
(int) 0 => array(
'id' => '1776',
'name' => 'product/kits/kit-icon.png',
'alt' => 'Kit icon',
'modified' => '2019-04-09 12:06:37',
'created' => '2018-03-15 15:51:02',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3158',
'antibody_id' => null,
'name' => '24 UDI for Tagmented libraries - Set I',
'description' => '<p><a href="https://www.diagenode.com/files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>The <strong>24 UDI for tagmented libraries</strong> includes 24 primer pairs for unique dual-indexing allowing the multiplexing of up to <b>24 samples </b>for sequencing on Illumina platforms. These UDI are designed and validated to be used with <a href="https://www.diagenode.com/en/p/uchipmentation-for-histones-24-rxns">μChIPmentation for Histones</a> (Cat. No. C01011011), <a href="https://www.diagenode.com/en/p/chipmentation-kit-for-histones">ChIPmentation Kit for Histones</a> (Cat. No. C01011009), <a href="https://www.diagenode.com/en/p/tag-kit-for-chipmentation-24">TAG Kit for ChIPmentation</a> (Cat. No. C01011030), <a href="https://www.diagenode.com/en/categories/atac-seq">ATAC-seq Kit</a> (Cat. No. C01080002). The 24 UDI for tagmented libraries are compatible with other <b>tagmentation</b><b>-based library preparation </b>protocols, such as <a href="https://www.diagenode.com/en/categories/cutandtag">CUT&Tag</a> technologies.</p>
<p>3 sets of UDI for tagmented libraries are available:</p>
<p><strong>24 UDI for tagmented libraries - Set I</strong><br /> <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set2">24 UDI for tagmented libraries - Set II</a><br /><a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set3" target="_blank">24 UDI for tagmented libraries - Set III</a><br /><br /></p>
<p><span>Each set can be used for library multiplexing up to 24. All sets can be used simultaneously for library multiplexing up to 72.</span></p>
<p>Features:</p>
<ul>
<li>Multiplexing: <b>up to 72 samples </b>(using all 3 sets simultaneously)<b><br /></b></li>
<li>Allow for <b>identification of index hopping</b></li>
<li>Compatibility: <b>tagmentation</b><b>-based library preparation protocols</b></li>
</ul>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => 'Characteristics',
'info1' => '<p>The <b>24 UDI (Unique dual indexes) for </b><b>tagmented</b><b> libraries – Set I </b>is compatible with any <b>tagmentation</b><b>-based library preparation </b>protocols, such as <strong>ChIPmentation</strong>, <b>ATAC-seq</b> or <b>CUT&Tag</b> technologies.</p>
<p>The <b>24 UDI for </b><b>tagmented</b><b> libraries </b>provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.</p>
<p></p>
<center><img src="https://www.diagenode.com/img/product/kits/UDI-for-tagmented-fig1.png" /></center>
<p></p>
<p><small><strong>Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries</strong> Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used. </small></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '24 UDI',
'catalog_number' => 'C01011034',
'old_catalog_number' => '',
'sf_code' => 'C01011034-',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '165',
'price_USD' => '185',
'price_GBP' => '155',
'price_JPY' => '28700',
'price_CNY' => '',
'price_AUD' => '465',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => '24-unique-dual-indexes-for-tagmented-libraries-set1',
'meta_title' => '24 unique dual indexes for tagmented libraries set 1',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2023-04-06 12:05:09',
'created' => '2020-12-08 15:26:23'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1110',
'name' => 'Primer indexes for tagmented libraries',
'description' => '<p class="p1">The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adaptors, previously incorporated in the libraries by tagmentation.</p>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/primer-indexes-for-tagmented-libraries_manual.pdf',
'slug' => 'primer-indexes-for-tagmented-libraries_manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-08-05 15:55:17',
'created' => '2020-12-08 16:29:19',
'ProductsDocument' => array(
'id' => '3042',
'product_id' => '3159',
'document_id' => '1110'
)
)
$sds = array(
'id' => '2130',
'name' => '8 UDI for tagmented libraries SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Tagmentase/SDS-C01011035-8_UDI_for_tagmented_libraries-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2021-10-12 14:48:42',
'created' => '2021-10-12 14:48:42',
'ProductsSafetySheet' => array(
'id' => '3689',
'product_id' => '3159',
'safety_sheet_id' => '2130'
)
)
$publication = array(
'id' => '4599',
'name' => 'In vitro production of cat-restricted Toxoplasma pre-sexual stages byepigenetic reprogramming',
'authors' => 'Antunes A. V.et al.',
'description' => '<p>Sexual reproduction of , which is restricted to the small intestine of felids, is sparsely documented, due to ethical concerns surrounding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described . In this study, we found that transcription factors AP2XII-1 and AP2XI-2, expressed in tachyzoite stage that causes acute toxoplasmosis, can silence genes necessary for merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. Pre-gametes produced under these conditions are characterized by specific protein markers and undergo typical asexual endopolygenic division cycles. In tachyzoites, AP2XII-1 and AP2XI-2 bind DNA as heterodimers at merozoite promoters and recruit the epigenitors MORC and HDAC3 , which in turn restrict the accessibility of chromatin to the transcriptional machinery. Thus, the commitment to merogony stems from a profound epigenetic rewiring orchestrated by AP2XII-1 and AP2XI-2. This effective culture of merozoites paves the way to explore sexual reproduction without the need to infect kittens and has potential for the development of therapeutics to block parasite transmission.</p>',
'date' => '2023-01-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36711883',
'doi' => '10.1101/2023.01.16.524187',
'modified' => '2023-04-06 09:52:08',
'created' => '2023-02-21 09:59:46',
'ProductsPublication' => array(
'id' => '6687',
'product_id' => '3159',
'publication_id' => '4599'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36711883" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
