Diagenode

8 UDI for tagmented libraries

Catalog Number
Format
Price
C01011035
8 UDI
$75.00
Other format

The 8 UDI (Unique dual indexes) for tagmented libraries provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

The 8 UDI for tagmented libraries includes 8 primer pairs for unique dual-indexing allowing the multiplexing of up to 8 samples for sequencing on Illumina platforms. This set of indexes is designed to be used with μChIPmentation for Histones (Cat. No. C01011012), but it is also compatible with other tagmentation-based library preparation protocols, such as ATAC-seq or CUT&Tag technologies.

  • Characteristics

    The 24 UDI (Unique dual indexes) for tagmented libraries – Set I is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.

    The 24 UDI for tagmented libraries provides combinations of barcodes where each barcode is uniquely attributed to one sample. This is a great tool to identify mistakes during index sequencing. A phenomenon, known as index hopping, can lead to misattribution of some reads to the wrong sample. This is particularly frequent with the NovaSeq6000, and thus the use of Unique Dual Indexing (UDI) is highly recommended when using this sequencer.

    Figure 1. Sequencing profiles of µChIPmentation libraries generated with 24 UDI for Tagmented libraries Chromatin preparation and immunoprecipitation have been performed on 10.000 cells using the µChIPmentation Kit for Histones (Cat. No. C01011011) and 24 UDI for Tagmented libraries – Set I (Cat. No. Cat. No. C01011034) using K562 cells. The Diagenode antibodies targeting H3K4me3 (Cat. No. C15410003) and rabbit IgG (Cat. No. C15410206) have been used.

  •  資料
    Primer indexes for tagmented libraries MANUAL
    The primer indexes for tagmented libraries are PCR primers targeting the Nextera sequencing adapt...
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  •  Safety sheets
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  •  出版物

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    Diagenode strongly recommends using this: 8 UDI for tagmented libraries (Diagenode Cat# C01011035). Click here to copy to clipboard.

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