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Chromatin EasyShear Kit – Ultra Low SDS

Catalog Number
Format
Price
C01020010
24 rxns
$305.00

Previous name of the kit: Chromatin shearing optimization kit – Ultra Low SDS

A high quality chromatin preparation is very complex and requires a lot of optimization. Chromatin EasyShear Kit – Ultra Low SDS is an optimized solution for efficient chromatin preparation prior to ChIP. The protocol, buffers composition, SDS concentration (<0.1%) is optimized for the preparation of chromatin prior to ChIP on histones. The kit has been validated with the Bioruptor ultrasonicator for efficient chromatin shearing, leading to chromatin fragments suitable for ChIP with the preserved epitopes. The kit is validated for cells and tissues.

Check all Chromatin EasyShear Kits.

Guide for the optimal chromatin preparation using Chromatin EasyShear Kits – Read more

  • Characteristics
    • Highly optimized protocol for chromatin preparation prior to ChIP on histones SDS concentration optimized for the workflow for histones
    • Validated for cells and tissue
    • Preserves the epitopes
    • Validated with the Bioruptor ultrasonicator
    • Quality of chromatin sample confirmed by ChIP-seq

    Figure 1. Optimal chromatin shearing profile
    HeLa cells were fixed with formaldehyde for 8 min and chromatin was prepared according to Diagenode’s Chromatin EasyShear Kit – Ultra Low SDS (Cat. No. C01020010). Samples were sonicated for 5-10-15 cycles of 30” ON/30” OFF as indicated with Bioruptor Pico using 1.5 ml Bioruptor microtubes with caps (Cat. No. C30010016) followed by de-crosslinking and DNA purification. The fragment size was assessed using agarose gel electrophoresis. A 100 bp ladder was loaded as the size standard.


    Figure 2. Chromatin precipitation
    Sheared chromatin (obtained with the Chromatin EasyShear Kit – Ultra Low SDS) has been used for immunoprecipitation with H3K4me3 and IgG (negative control) antibodies. Quantitative PCR was performed with positive (GAPDH) and negative (TSH2B) control regions. The Figure 2 shows the recovery expressed as % of input (the relative amount of immunoprecipitated DNA compared to input DNA (panel A) and as enrichment fold of positive locus over negative (panel B).

  • Cells tested

    The Chromatin EasyShear Kit is compatible with a broad variety of cell lines, tissues and species – some examples are shown below. Other species / cell lines / tissues can be used with this kit.

    Cell lines:

    • Human: A549, A673, CD8+ T, Blood vascular endothelial cells, Lymphatic endothelial cells, fibroblasts, K562, MDA-MB231
    • Mouse: C2C12, primary HSPC, synovial fibroblasts, HeLa-S3, FACS sorted cells from embryonic kidneys, macrophages, mesodermal cells, myoblasts, NPC, salivary glands, spermatids, spermatocytes, skeletal muscle stem cells, stem cells, Th2
    • Hamster: CHO
    • Other cell lines/speices: compatible, not tested

    Tissue:

    • Bee – brain
    • Daphnia – whole animal
    • Horse – brain, heart, lamina, liver, lung, skeletal muscles, ovary
    • Human – Erwing sarcoma tumor samples
    • Other tissues: compatible, not tested

    Did you use the Chromatin EasyShear Kit on other cell line / tissue / species? Let us know!

  •  Documents
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
    Download
    Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones) MANUAL
    The first critical step of a successful Chromatin immunoprecipitation (Chip...
    Download
  •  Safety sheets
    Chromatin EasyShear Kit - Ultra Low SDS US en Download
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Chromatin EasyShear Kit – Ultra Low SDS (Diagenode Cat# C01020010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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