Diagenode

pA-Tn5 Transposase - loaded

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  •  Description
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Catalog Number
Format
C01070001
15 µl (32 rxns)
Other format


pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For convenience, the fusion protein is pre-loaded with sequencing adapters. Diagenode pA-Tn5 transposase is compatible with CUT&Tag and other antibody-tethered Tn5 based methods, like ACT-seq and CoBATCH.

pA-Tn5 Transposase

  • High enrichment and specificity of the signal around the TSS
  • No contamination with E. coli DNA
  • Flexible versions: loaded or unloaded
  • Excellent for CUT&Tag

As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.

Check out all products recommended for CUT&Tag assay:

TESTIMONIAL

We are really happy to use the loaded pA-Tn5 for CUT&Tag. Easy to use and reproductible for samples with a starting low number of cells. Besides, Diagenode has a great customer service and technical support which are really responsive.

Cathy NGUYEN, CNRGH, Evry-Courcouronnes, France.
  • Validation data
    A.
    pA-Tn5 Transposase loaded  H3K4me3 Validation

    B.
    pA-Tn5 Transposase loaded  H3K9me3 Validation

    C.
    pA-Tn5 Transposase loaded H3K27me3 Validation

    Figure 1. Representative screenshots for H3K4me3 (A), H3K9me3 (B) and H3K27me3 (C) data obtained using Diagenode pA-Tn5 fusion protein (C01070001) and CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)) at selected loci. CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) and H3K9me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410193).

    pA-Tn5 Transposase loaded H3K27me3 Validation
    pA-Tn5 Transposase loaded H3K4me3 Validation

    Figure 2. CUT&Tag profiles show typical enrichments specific for a given histone mark. Representative screenshots for H3K27me3 (red) and H3K4me3 (green) are shown at selected loci. H3K27me3, which marks inactive regions (red), does not show enrichment over open chromatin regions, marked by H3K4me3 (green). CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), and H3K27me3 polyclonal ChIP-seq grade (Cat. No.C15410195).

  •  Documents
    pA-TN5 transposase (loaded) datasheet DATASHEET
    pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
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  •  Safety sheets
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: pA-Tn5 Transposase - loaded (Diagenode Cat# C01070001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Transcription factor EGR2 controls homing and pathogenicity of T17cells in the central nervous system.
    Gao Y. et al.
    CD4 T helper 17 (T17) cells protect barrier tissues but also trigger autoimmunity. The mechanisms behind these opposing processes remain unclear. Here, we found that the transcription factor EGR2 controlled the transcriptional program of pathogenic T17 cells in the central nervous system (CNS) but not that of protec...

    The Hdc GC box is critical for Hdc gene transcription andhistamine-mediated anaphylaxis.
    Li Y. et al.
    BACKGROUND: Histamine is a critical mediator of anaphylaxis, a neurotransmitter, and a regulator of gastric acid secretion. Histidine decarboxylase is a rate-limiting enzyme for histamine synthesis. However, in vivo regulation of Hdc, the gene that encodes histidine decarboxylase is poorly understood. OBJECTIVE: We ...

    Retrospective analysis of enhancer activity and transcriptome history.
    Boers R. et al.
    Cell state changes in development and disease are controlled by gene regulatory networks, the dynamics of which are difficult to track in real time. In this study, we used an inducible DCM-RNA polymerase subunit b fusion protein which labels active genes and enhancers with a bacterial methylation mark that does not ...

    Simultaneous profiling of histone modifications and DNA methylation viananopore sequencing.
    Yue X. et al.
    The interplay between histone modifications and DNA methylation drives the establishment and maintenance of the cellular epigenomic landscape, but it remains challenging to investigate the complex relationship between these epigenetic marks across the genome. Here we describe a nanopore-sequencing-based-method, nano...

    A SOX2-engineered epigenetic silencer factor represses the glioblastomagenetic program and restrains tumor development.
    Benedetti V. et al.
    Current therapies remain unsatisfactory in preventing the recurrence of glioblastoma multiforme (GBM), which leads to poor patient survival. By rational engineering of the transcription factor SOX2, a key promoter of GBM malignancy, together with the Kruppel-associated box and DNA methyltransferase3A/L catalytic dom...

    Caffeine intake exerts dual genome-wide effects on hippocampal metabolismand learning-dependent transcription.
    Paiva I. et al.
    Caffeine is the most widely consumed psychoactive substance in the world. Strikingly, the molecular pathways engaged by its regular consumption remain unclear. We herein addressed the mechanisms associated with habitual (chronic) caffeine consumption in the mouse hippocampus using untargeted orthogonal omics techniq...

    ATRX regulates glial identity and the tumor microenvironment inIDH-mutant glioma
    Babikir, Husam and Wang, Lin and Shamardani, Karin andCatalan, Francisca and Sudhir, Sweta and Aghi, Manish K. andRaleigh, David R. and Phillips, Joanna J. and Diaz, AaronA.
    Background Recent single-cell transcriptomic studies report that IDH-mutant gliomas share a common hierarchy of cellular phenotypes, independent of genetic subtype. However, the genetic differences between IDH-mutant glioma subtypes are prognostic, predictive of response to chemotherapy, and correlate with distinct ...

    Autocrine Vitamin D-signaling switches off pro-inflammatory programsof Th1 cells
    Chauss D.et al.
    The molecular mechanisms governing orderly shutdown and retraction of CD4+ T helper (Th)1 responses remain poorly understood. Here, we show that complement triggers contraction of Th1 responses by inducing intrinsic expression of the vitamin D (VitD) receptor (VDR) and the VitD-activating enzyme CYP27B1, permitting ...

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