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pA-Tn5 Transposase - loaded

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カタログ番号
フォーマット
C01070001
15 µl (32 rxns)
ブローカー情報

pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For convenience, the fusion protein is pre-loaded with sequencing adapters.

As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.

Check out all products recommended for CUT&Tag assay:

  • Validation data
    A.
    pA-Tn5 Transposase loaded  H3K4me3 Validation

    B.
    pA-Tn5 Transposase loaded  H3K9me3 Validation

    C.
    pA-Tn5 Transposase loaded H3K27me3 Validation

    Figure 1. Representative screenshots for H3K4me3 (A), H3K9me3 (B) and H3K27me3 (C) data obtained using Diagenode pA-Tn5 fusion protein (C01070001) and CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)) at selected loci. CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) and H3K9me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410193).

    pA-Tn5 Transposase loaded H3K27me3 Validation
    pA-Tn5 Transposase loaded H3K4me3 Validation

    Figure 2. CUT&Tag profiles show typical enrichments specific for a given histone mark. Representative screenshots for H3K27me3 (red) and H3K4me3 (green) are shown at selected loci. H3K27me3, which marks inactive regions (red), does not show enrichment over open chromatin regions, marked by H3K4me3 (green). CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), and H3K27me3 polyclonal ChIP-seq grade (Cat. No.C15410195).

  •  資料
    pA-TN5 transposase (loaded) datasheet DATASHEET
    pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
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  •  Safety sheets
    pA-Tn5 Transposase - loaded SDS US en Download
    pA-Tn5 Transposase - loaded SDS GB en Download
    pA-Tn5 Transposase - loaded SDS BE fr Download
    pA-Tn5 Transposase - loaded SDS FR fr Download
    pA-Tn5 Transposase - loaded SDS ES es Download
    pA-Tn5 Transposase - loaded SDS DE de Download
    pA-Tn5 Transposase - loaded SDS JP ja Download
    pA-Tn5 Transposase - loaded SDS BE nl Download
  •  出版物

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