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pA-Tn5 Transposase - loaded

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カタログ番号
フォーマット
C01070001
15 µl (32 rxns)
ブローカー情報

pA-Tn5トランスポザーゼは、CUT&Tagアッセイ専用に開発された高活性Tn5トランスポザーゼとプロテインAの融合タンパク質です。 融合タンパク質にはシーケンスアダプターがあらかじめロードされある、便利なプリロードです。

目的のタンパク質のみをターゲットとすることが重要であるため、CUT&Tagアッセイの成功には非常に特異的な抗体が不可欠です。 厳密な検証基準を満たしている特異性が検証されているDiagenode ChIP-seqグレードの抗体は、CUT&Tagアッセイでの高いパフォーマンスを保証します。

CUT&Tagアッセイに関する推奨製品はこちら

  • 検証データ
    A.
    pA-Tn5 Transposase loaded  H3K4me3 Validation

    B.
    pA-Tn5 Transposase loaded  H3K9me3 Validation

    C.
    pA-Tn5 Transposase loaded H3K27me3 Validation

    Figure 1. Representative screenshots for H3K4me3 (A), H3K9me3 (B) and H3K27me3 (C) data obtained using Diagenode pA-Tn5 fusion protein (C01070001) and CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)) at selected loci. CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) and H3K9me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410193).

    pA-Tn5 Transposase loaded H3K27me3 Validation
    pA-Tn5 Transposase loaded H3K4me3 Validation

    Figure 2. CUT&Tag profiles show typical enrichments specific for a given histone mark. Representative screenshots for H3K27me3 (red) and H3K4me3 (green) are shown at selected loci. H3K27me3, which marks inactive regions (red), does not show enrichment over open chromatin regions, marked by H3K4me3 (green). CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), and H3K27me3 polyclonal ChIP-seq grade (Cat. No.C15410195).

  •  資料
    pA-TN5 transposase (loaded) datasheet DATASHEET
    pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
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  •  Safety sheets
    pA-Tn5 Transposase - loaded SDS GB en Download
    pA-Tn5 Transposase - loaded SDS US en Download
    pA-Tn5 Transposase - loaded SDS BE nl Download
    pA-Tn5 Transposase - loaded SDS BE fr Download
    pA-Tn5 Transposase - loaded SDS FR fr Download
    pA-Tn5 Transposase - loaded SDS ES es Download
    pA-Tn5 Transposase - loaded SDS DE de Download
    pA-Tn5 Transposase - loaded SDS JP ja Download
  •  出版物

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