Diagenode

Caffeine intake exerts dual genome-wide effects on hippocampal metabolismand learning-dependent transcription.


Paiva I. et al.

Caffeine is the most widely consumed psychoactive substance in the world. Strikingly, the molecular pathways engaged by its regular consumption remain unclear. We herein addressed the mechanisms associated with habitual (chronic) caffeine consumption in the mouse hippocampus using untargeted orthogonal omics techniques. Our results revealed that chronic caffeine exerts concerted pleiotropic effects in the hippocampus at the epigenomic, proteomic, and metabolomic levels. Caffeine lowered metabolism-related processes (e.g., at the level of metabolomics and gene expression) in bulk tissue, while it induced neuron-specific epigenetic changes at synaptic transmission/plasticity-related genes and increased experience-driven transcriptional activity. Altogether, these findings suggest that regular caffeine intake improves the signal-to-noise ratio during information encoding, in part through fine-tuning of metabolic genes, while boosting the salience of information processing during learning in neuronal circuits.

Tags
Antibody
Microplex Library Preparation kit
CUT&Tag

Share this article

Published
June, 2022

Source

Products used in this publication

  • cut and tag antibody icon
    C15410200
    H3K9/14ac polyclonal antibody
  • default alt
    C03040001
    MicroChIP DiaPure columns
  • chip kit icon
    C05010001
    MicroPlex Library Preparation Kit v3
  • ChIP kit icon
    C05010012
    MicroPlex Library Preparation Kit v2 (12 indices)
  • cut and tag antibody icon
    C15410195
    H3K27me3 polyclonal antibody
  • default alt
    C01070001
    pA-Tn5 Transposase - loaded
  • Mouse IgG
    C15410206
    Rabbit IgG

イベント

  • APHL 2024
    Milwaukee, Wisconsin, USA
    May 6-May 9, 2024
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics