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24 SI for ChIPmentation

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Catalog Number
Format
Price
C01011031
24 indexes
$170.00

The 24 SI for ChIPmentation includes 24 single primer indexes completing the TAG Kit for ChIPmentation. This set of indexes allows for multiplexing up to 24 samples. The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol. Our Kit TAG for ChIPmentation includes all reagents necessary for tagmentation-based library preparation compatible with any ChIP protocol based on magnetic beads.

The primer indexes of the 24 SI for ChIPmentation are also compatible with other tagmentation-based library preparation protocols, such as ATAC-seq or CUT&Tag technologies.

  • Characteristics

    Comparing to classical ChIP-seq, ChIPmentation enables:

    • an easier protocol
    • faster time to results
    • flexibility – the TAG Kit for ChIPmentation combained with 24 SI for ChIPmentation allows to perform ChIPmentation with any ChIP protocol (based on magnetic beads)

    ChIPmentation on histone marks using TAG KIT for ChIPmentation

    ChIPmentation sequencing results for four histone marks. Chromatin preparation has been performed on 7 M K562 cells using the Auto iDeal ChIP-seq Kit for Histones (Cat. No. C01010057), TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031)*. Diluted chromatin from 1,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting H3K4me1 (Cat. No. C15410194), H3K27me3 (Cat. No. C15410195), H3K27ac (Cat. No. C15410196) and IgG (Cat. No. C15410206). The experiment was performed on the IP-Star® Compact Automated System.

    *The kits Auto iDeal ChIP-seq for Histones (C01010057), TAG Kit for ChIPmentation (C01011030) and 24 SI for ChIPmentation (C01011031) form together the Auto ChIPmentation Kit for Histones* (C01011000).

    ChIPmentation on non-histone proteins using TAG Kit for ChIPmentation

    ChIPmentation sequencing results for two non histone factors. Chromatin preparation has been performed on 25M K562 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Diluted chromatin from 1,000,000 or 4,000,000 cells was used for the immunoprecipitation with the Diagenode antibodies targeting CTCF (Cat. No. C15410210) and RNA PolII (Cat. No. C15100055 ) respectively. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).

  •  Testimonials

    We were very happy with the method.  It gave good results in the end, and required much smaller samples than we need to reliably perform conventional ChIP-seq.
    In our view, the main advantages of the ChIPmentation kit compared to our conventional ChIP-seq protocol are (most important first):

    • smaller sample requirement,
    • simpler workflow with less that can go wrong,
    • slightly higher resolution and signal: noise ratio.

    ChIPmentation sequencing profiles for p65. Chromatin preparation and immunoprecipitation have been performed on stimulated NIH3T3 cells using the iDeal ChIP-seq kit for TFs (Cat. No. C01010055). Chromatin from 4,000,000 cells was used for the immunoprecipitation in combination with either anti-p65 antibody or IgG. The library preparation was performed with the TAG Kit for ChIPmentation (Cat. No. C01011030) and 24 SI for ChIPmentation (Cat. No. C01011031).

    Researcher from University of Nice-Sophia Antipolis, Nice, France
  •  Documents
    24 SI for ChIPmentation MANUAL
    The 24 SI for ChIPmentation includes 24 single primer indexes allowing the multiplexing of up to ...
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  •  Safety sheets
    24 SI for ChIPmentation SDS US en Download
    24 SI for ChIPmentation SDS GB en Download
    24 SI for ChIPmentation SDS ES es Download
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    24 SI for ChIPmentation SDS BE nl Download
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    24 SI for ChIPmentation SDS FR fr Download
  •  Publications

    How to properly cite this product in your work

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