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pA-Tn5 Transposase - unloaded

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Catalog Number
Format
C01070002
10 µl
Other format

pA-Tn5トランスポザーゼは、CUT&Tagアッセイ専用に開発された高活性Tn5トランスポザーゼとプロテインAの融合タンパク質です。融通性を重視し、融合タンパク質にはシーケンスアダプターがロードされていません。 ご使用前に、推奨されるプロトコールに従って組み立ててください。

目的のタンパク質のみをターゲットとすることが重要であるため、CUT&Tagアッセイの成功には非常に特異的な抗体が不可欠です。 厳密な検証基準を満たしている特異性が検証されているDiagenode ChIP-seqグレードの抗体は、CUT&Tagアッセイでの高いパフォーマンスを保証します。

CUT&Tagアッセイに関する推奨製品はこちら

  • 検証データ
    A.
    pA-Tn5 Transposase
    B.
    pA-Tn5 Transposase unloaded

    Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters

    A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.

    B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).

  •  資料
    Transposome Assembly Using Diagenode pA-Tn5 Transposase PROTOCOL
    pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and pr...
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    pA-TN5 transposase (unloaded) datasheet DATASHEET
    pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
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  •  Safety sheets
    pA-Tn5 Transposase - unloaded SDS GB en Download
    pA-Tn5 Transposase - unloaded SDS US en Download
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    pA-Tn5 Transposase - unloaded SDS BE fr Download
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    pA-Tn5 Transposase - unloaded SDS ES es Download
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    pA-Tn5 Transposase - unloaded SDS JP ja Download
  •  出版物

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    Spatial epigenome-transcriptome co-profiling of mammalian tissues.
    Zhang D. et al.
    Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relatio...

    Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level
    Fan Rong et al.
    Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship ac...

    Spatially resolved epigenomic profiling of single cells in complex tissues
    Lu Tian et al.
    The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissu...

    PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation
    Danyan Li, David H Taylor, Josien C van Wolfswinkel
    PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate inte...

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