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pA-Tn5 Transposase - loaded

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Catalog Number
Format
C01070001
15 µl (32 rxns)
Other format

pA-Tn5 Transposase loaded

pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For convenience, the fusion protein is pre-loaded with sequencing adapters. Diagenode pA-Tn5 transposase is compatible with CUT&Tag and other antibody-tethered Tn5 based methods, like ACT-seq and CoBATCH.

pA-Tn5 Transposase

  • High enrichment and specificity of the signal around the TSS
  • No contamination with E. coli DNA
  • Flexible versions: loaded or unloaded
  • Excellent for CUT&Tag

As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.

Check out all products recommended for CUT&Tag assay:

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  • Validation data
    A.
    pA-Tn5 Transposase loaded  H3K4me3 Validation

    B.
    pA-Tn5 Transposase loaded  H3K9me3 Validation

    C.
    pA-Tn5 Transposase loaded H3K27me3 Validation

    Figure 1. Representative screenshots for H3K4me3 (A), H3K9me3 (B) and H3K27me3 (C) data obtained using Diagenode pA-Tn5 fusion protein (C01070001) and CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)) at selected loci. CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) and H3K9me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410193).

    pA-Tn5 Transposase loaded H3K27me3 Validation
    pA-Tn5 Transposase loaded H3K4me3 Validation

    Figure 2. CUT&Tag profiles show typical enrichments specific for a given histone mark. Representative screenshots for H3K27me3 (red) and H3K4me3 (green) are shown at selected loci. H3K27me3, which marks inactive regions (red), does not show enrichment over open chromatin regions, marked by H3K4me3 (green). CUT&Tag was performed using 50,000 of K562 cells and Diagenode H3K4me3 polyclonal ChIP-seq antibody (Cat. No. C15410003), and H3K27me3 polyclonal ChIP-seq grade (Cat. No.C15410195).

  •  Documents
    pA-TN5 transposase (loaded) datasheet DATASHEET
    pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
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  •  Publications

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