pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).
pA-Tn5 Transposase
High enrichment and specificity of the signal around the TSS
Excellent for CUT&Tag and similar assays (eg. TIP-seq)
As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.
Check out all products recommended for CUT&Tag assay:
Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters
A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.
B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).
Diagenode strongly recommends using this: pA-Tn5 Transposase - unloaded (Diagenode Cat# C01070002). Click here to copy to clipboard.
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Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level Fan Rong et al. Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship ac...
Spatially resolved epigenomic profiling of single cells in complex tissues Lu Tian et al. The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissu...
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