pA-Tn5 Transposase - unloaded

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10 µl
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pA-Tn5 Transposase unloaded

pA-Tn5 Transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).

pA-Tn5 Transposase

  • High enrichment and specificity of the signal around the TSS
  • No contamination with E. coli DNA
  • Flexible versions: loaded or unloaded
  • Excellent for CUT&Tag and similar assays (eg. TIP-seq)

As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.

Check out all products recommended for CUT&Tag assay:

  • Validation data
    pA-Tn5 Transposase
    pA-Tn5 Transposase unloaded

    Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters

    A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.

    B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. C15410195) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).

  •  Documents
    Transposome Assembly Using Diagenode pA-Tn5 Transposase PROTOCOL
    pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and pr...
    pA-TN5 transposase (unloaded) datasheet DATASHEET
    pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for...
  •  Publications

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