Diagenode

エピジェネティクス・クロマチン解析

ChIP-seq

検出方法(ChIP-seq)としてハイスループットシーケンシングと結合したクロマチン免疫沈降(ChIP)は、今やエピゲノム研究、すなわちゲノム規模でのタンパク質-DNA相互作用の研究において主要な方法の1つされています。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。
ChIP-seqワークフロー


  1. クロマチン調製 : DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定(架橋)に続いて細胞溶解。
  2. クロマチン断片化 :  超音波処理による所望の断片サイズ(100500bp)までのクロマチンの断片化
  3. クロマチン免疫沈降 : 目的のヒストンまたは転写因子に対する特定のChIP級抗体を用いたタンパク質-DNA複合体の捕捉
  4. DNA精製: クロマチン逆架橋および溶出後の精製
  5. NGSライブラリ調整 : アダプターを結紮してIP化されたサンプルを増幅
  6. バイオインフォマティクス分析 : リードフィルタリングとトリミング、リードによるアライメント、エンリチッメントによるピークコール、QCメトリック、マルチサンプルの相互比較などを実行します。

さらに詳しく

当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。

Recommended products for ChIP-seq

Cat. No.ProductFormatPrice
C01011000 ChIPmentation for Histones
Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation, incorporates automation wi...
24 rxns $2,320.00
C01010171 Auto iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns $2,070.00
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns $2,070.00
C01010140 Auto True MicroChIP kit
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This m...
16 rxns $510.00
C01010153 Auto Universal Plant ChIP-seq kit
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns $510.00
B01060010 Picoruptor ® ソニケーター
Picoruptor は剪断の最新の技術革新であり、最大5μlから最大2mlまでの剪断マイクロボリュームのために最適化されたオールインワン剪断システムとしての新たなブレークスルーを表しています。 冷却システム(ウォータークーラーとシングルサイクルバルブ)により、高精度の温度制御が可能になり、より高...
1 unit
C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective in cross-l...
600 µl $130.00
C01010051 iDeal ChIP-seq kit for Histones
もう貴重なシーケンシングサンプルを無駄にする必要はありません。 Diagenodeの検証済みiDeal ChIP-seq kitは必要なものすべてを備えています。iDeal ChIP-seq kitは、業界で唯一GAIIx(Illumina®)およびPGM™(Ion Torrent&trade...
24 rxns $1,030.00
C01010055 iDeal ChIP-seq kit for Transcription Factors
DiagenodeのiDeal ChIP-seq for Transcription Factorsキットは、安定した転写因子ChIP-seqの結果を実現するために、特殊化されたソリューションです。 競合する他社のソリューションとは異なり、本キットは高度に最適化されたプロトコルを使用しており、その品質は多数の転写...
24 rxns $1,030.00
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
C01010130 True MicroChIP kit
True MicroChIP kitとMicroPlex Library Preparation™kitを組み合わせることにより、わずか10,000細胞でChIP-seqアッセイが実行できます。このmicroChIP-seqアッセイは、新世代のIP-Star® Compact Automate...
16 rxns $510.00
C01010152 Universal Plant ChIP-seq kit
Universal Plant ChIP-seqは、シロイヌナズナ、トウモロコシ、イネ、トマト、ポプラなどから、植物クロマチンを抽出できる非常に便利なキットです。 このキットは植物染色体抽出用に特別開発されており、染色質調製、免疫沈降、植物特異的コントロールプライマーペア、コントロール抗体、DNA精製のための試薬...
24 rxns $510.00
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc $40.00
C30080030 96 well microplates for IP-Star®
Standard 96 well transparent plates made by polyestyrene and compatible with automated systems. Solid U-bottom plates free of DNAase and RNAase and human DNA.
10 pc $70.00
C01010058 Auto iDeal ChIP-seq Kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
24 rxns $1,030.00
C03010010 Auto IPure kit v2
The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the o...
100 rxns $360.00
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns $510.00
C01010141 Auto True MicroChIP & MicroPlex Library Preparation™ Package
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation kit allows for performing ChIP-seq on as few as 10,000 cells. Thi...
16 ChIP rxns and 12 library prep rxns $1,610.00
C15410337 BRD4 polyclonal antibody
Polyclonal antibody raised in rabbit against human BRD4 (Bromodomain Containing 4), using two KLH-conjugated synthetic peptides from the N-terminal and centr...
50 µg $305.00
C01010053 iDeal ChIP-seq for Histones & Library Preparation Package (incl. Index Primer Set 1)
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns $1,960.00
C01010056 iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike comp...
10 ChIP and 12 library prep rxns $1,610.00
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $245.00
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns $245.00
C03010015 IPure kit v2
High DNA recovery after ChIP or MeDIP is critical for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only D...
100 rxns $360.00
C17040004 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl $35.00
C17040005 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl $35.00
C17040011 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl $35.00
C17040010 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl $35.00
C17040009 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl $35.00
C17040006 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $35.00
C17040007 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl $35.00
C01010131 True MicroChIP & MicroPlex Library Preparation™ Package
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microC...
16 ChIP rxns and 12 library prep rxns $1,610.00

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