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ChIP Cross-link Gold

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カタログ番号
フォーマット
価格
C01019027
600 µl
$135.00

架橋(クロスリンキング)は、一般的には可逆的DNA-タンパク質結合を形成するホルムアルデヒドを用いて行われます。 しかし、通常のホルムアルデヒドはDNAに直接結合していないタンパク質を架橋するには有効ではありません。 例えば、誘導性転写因子または補因子は、タンパク質 - タンパク質相互作用を介してDNAと相互作用し、ホルムアルデヒドを用いても、これらを十分に保存することは出来ません。このような高次および動的相互作用には効率的なタンパク質 - タンパク質安定化に適した他の架橋剤を ホルムアルデヒドと組み合わせて使用することで、最良の結果を得ることが出来ます。その組み合わせとして最適なDiagenodeのChIPクロスリンクゴールドは高次タンパク質相互作用において有効的で最良の選択肢です。

  •  プロトコル集
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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  •  資料
    ChIP cross-link Gold MANUAL
    The first step of a ChIP assay is the cross-linking in order to fix the cells. Cross-linkin...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ChIP Cross-link Gold (Diagenode Cat# C01019027). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A stress-responsive enhancer induces dynamic drug resistance in acute myeloid leukemia.
    Williams MS, Amaral FM, Simeoni F, Somervaille TC
    The drug efflux pump ABCB1 is a key driver of chemoresistance, and high expression predicts for treatment failure in acute myeloid leukemia (AML). In this study, we identified and functionally validated the network of enhancers that controls expression of ABCB1. We show that exposure of leukemia cells to daunorubici...

    The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress.
    Moreno Leon L, Gautier M, Allan R, Ilié M, Nottet N, Pons N, Paquet A, Lebrigand K, Truchi M, Fassy J, Magnone V, Kinnebrew G, Radovich M, Cheok MH, Barbry P, Vassaux G, Marquette CH, Ponzio G, Ivan M, Pottier N, Hofman P, Mari B, Rezzonico R
    Lung cancer is the leading cause of cancer death worldwide, with poor prognosis and a high rate of recurrence despite early surgical removal. Hypoxic regions within tumors represent sources of aggressiveness and resistance to therapy. Although long non-coding RNAs (lncRNAs) are increasingly recognized as major gene ...

    P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.
    Bugai A, Quaresma AJC, Friedel CC, Lenasi T, Düster R, Sibley CR, Fujinaga K, Kukanja P, Hennig T, Blasius M, Geyer M, Ule J, Dölken L, Barborič M
    DNA damage response (DDR) involves dramatic transcriptional alterations, the mechanisms of which remain ill defined. Here, we show that following genotoxic stress, the RNA-binding motif protein 7 (RBM7) stimulates RNA polymerase II (Pol II) transcription and promotes cell viability by activating the positive transcr...

    The BRG1/SOX9 axis is critical for acinar cell-derived pancreatic tumorigenesis.
    Tsuda M, Fukuda A, Roy N, Hiramatsu Y, Leonhardt L, Kakiuchi N, Hoyer K, Ogawa S, Goto N, Ikuta K, Kimura Y, Matsumoto Y, Takada Y, Yoshioka T, Maruno T, Yamaga Y, Kim GE, Akiyama H, Ogawa S, Wright CV, Saur D, Takaori K, Uemoto S, Hebrok M, Chiba T, Seno
    Chromatin remodeler Brahma related gene 1 (BRG1) is silenced in approximately 10% of human pancreatic ductal adenocarcinomas (PDAs). We previously showed that BRG1 inhibits the formation of intraductal pancreatic mucinous neoplasm (IPMN) and that IPMN-derived PDA originated from ductal cells. However, the role of BR...

    A new metabolic gene signature in prostate cancer regulated by JMJD3 and EZH2.
    Daures M, Idrissou M, Judes G, Rifaï K, Penault-Llorca F, Bignon YJ, Guy L, Bernard-Gallon D
    Histone methylation is essential for gene expression control. Trimethylated lysine 27 of histone 3 (H3K27me3) is controlled by the balance between the activities of JMJD3 demethylase and EZH2 methyltransferase. This epigenetic mark has been shown to be deregulated in prostate cancer, and evidence shows H3K27me3 enri...

    SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription
    Tran N.T. et al.
    The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the...

    EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells
    Beringer M. et al.
    The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding fact...

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