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Universal Plant ChIP-seq kit

カタログ番号
フォーマット
価格
C01010152
24 rxns
$510.00

Universal Plant ChIP-seqは、シロイヌナズナ、トウモロコシ、イネ、トマト、ポプラなどから、植物クロマチンを抽出できる非常に便利なキットです。 このキットは植物染色体抽出用に特別開発されており、染色質調製、免疫沈降、植物特異的コントロールプライマーペア、コントロール抗体、DNA精製のための試薬が含まれています。

  • 特徴
    • Universal compatiblity with a wide variety of plant species
    • Optimized and complete kit for start-to-finish plant ChIP
    • Includes plant-specific control primers and control antibody

    Successful ChIP-seq experiments for a variety of plants

    Arabidopsis

    Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.

    Poplar

    Figure 3. ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

    Tomato

    Figure 2. ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.

    Maize

    Figure 4. ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

     

    Plant Species

    Validated antibodies

    Validated primer pairs

    Arabidopsis (Arabidopsis thaliana) H3K4me3 polyclonal antibody - Premium Arabidopsis Actin ATG primer pair
    H3K4me3 monoclonal antibody - Classic Arabidopsis FLC-ATG primer pair
    H3K9me2 polyclonal antibody - Classic Arabidopsis FLC-intron1 primer pair
    H3K9me3 polyclonal antibody - Classic
    H3K9/14ac polyclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium
    H3K27ac polyclonal antibody - Premium
    H3K36me3 polyclonal antibody - Premium
    Maize (Zea mays) H3K4me3 polyclonal antibody - Premium Maize B73 inner stem ZmB1-UTR primer pair
    H3K27me3 polyclonal antibody - Premium Maize B73 inner stem ZmCopia primer pair
    H3pan monoclonal antibody - Classic
    Tomato (Solanum lycopersicum) H3K4me3 polyclonal antibody - Premium Tomato leaves SlChr2-reg8 primer pair
    H3K9me2 polyclonal antibody - Classic Tomato leaves SlChr4-NC1 primer pair
    H3K27me3 polyclonal antibody - Premium
    H3pan monoclonal antibody - Classic
    Rice (Oriza sativa) H3K4me3 polyclonal antibody - Premium Rice seedlings OsChr4-reg9 primer pair
    H3K9me2 polyclonal antibody - Classic Rice seedlings OsMADS6 primer pair
    H3K36me3 polyclonal antibody - Premium
    Poplar (Populus trichocarpa, Populus tremula x alba) H3K4me3 polyclonal antibody - Premium Poplar xylem PtrCopia-orth primer pair
    H3K9ac polyclonal antibody - Classic Poplar xylem PtrMYBTF1 primer pair
    H3pan monoclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium

  •  資料
    Universal Plant ChIP-seq kit MANUAL
    Manual of the Universal Plant ChIP-seq Kit
    Download
    Cell number estimation plants XLS FILE
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Universal Plant ChIP-seq kit (Diagenode Cat# C01010152). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    REDOX RESPONSIVE TRANSCRIPTION FACTOR1 (RRFT1) is involved in extracellular ATP regulated Arabidopsis thaliana seedling growth.
    Zhu R, Dong X, Xue Y, Xu J, Zhang A, Feng M, Zhao Q, Xia S, Yin Y, He S, Li Y, Liu T, Kang E, Shang Z
    Extracellular ATP (eATP) is an apoplastic signaling molecule that plays essential roles in the growth and development of plants. Arabidopsis seedlings have been reported to respond to eATP, however, the downstream signaling components are still not well understood. Here, we report that an ethylene responsive factor,...

    An inferred fitness consequence map of the rice genome.
    Joly-Lopez Z, Platts AE, Gulko B, Choi JY, Groen SC, Zhong X, Siepel A, Purugganan MD
    The extent to which sequence variation impacts plant fitness is poorly understood. High-resolution maps detailing the constraint acting on the genome, especially in regulatory sites, would be beneficial as functional annotation of noncoding sequences remains sparse. Here, we present a fitness consequence (fitCons) m...

    Characterization of the Polycomb-Group Mark H3K27me3 in Unicellular Algae
    Mikulski P. et al.
    Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and ...

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