Diagenode

Universal Plant ChIP-seq kit

ChIP kit icon
Catalog Number
Format
Price
C01010152
24 rxns
$510.00

The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for plant chromatin extraction and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.

  • Characteristics
    • Universal compatiblity with a wide variety of plant species
    • Optimized and complete kit for start-to-finish plant ChIP
    • Includes plant-specific control primers and control antibody

    Successful ChIP-seq experiments for a variety of plants

    Arabidopsis

    Figure 1. ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.

    Poplar

    Figure 3. ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

    Tomato

    Figure 2. ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina® HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.

    Maize

    Figure 4. ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Libraries were prepared with our MicroPlex Library Preparation™ kit from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina® HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.

     

    Plant Species

    Validated antibodies

    Validated primer pairs

    Arabidopsis (Arabidopsis thaliana) H3K4me3 polyclonal antibody - Premium Arabidopsis Actin ATG primer pair
    H3K4me3 monoclonal antibody - Classic Arabidopsis FLC-ATG primer pair
    H3K9me2 polyclonal antibody - Classic Arabidopsis FLC-intron1 primer pair
    H3K9me3 polyclonal antibody - Classic
    H3K9/14ac polyclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium
    H3K27ac polyclonal antibody - Premium
    H3K36me3 polyclonal antibody - Premium
    Maize (Zea mays) H3K4me3 polyclonal antibody - Premium Maize B73 inner stem ZmB1-UTR primer pair
    H3K27me3 polyclonal antibody - Premium Maize B73 inner stem ZmCopia primer pair
    H3pan monoclonal antibody - Classic
    Tomato (Solanum lycopersicum) H3K4me3 polyclonal antibody - Premium Tomato leaves SlChr2-reg8 primer pair
    H3K9me2 polyclonal antibody - Classic Tomato leaves SlChr4-NC1 primer pair
    H3K27me3 polyclonal antibody - Premium
    H3pan monoclonal antibody - Classic
    Rice (Oriza sativa) H3K4me3 polyclonal antibody - Premium Rice seedlings OsChr4-reg9 primer pair
    H3K9me2 polyclonal antibody - Classic Rice seedlings OsMADS6 primer pair
    H3K36me3 polyclonal antibody - Premium
    Poplar (Populus trichocarpa, Populus tremula x alba) H3K4me3 polyclonal antibody - Premium Poplar xylem PtrCopia-orth primer pair
    H3K9ac polyclonal antibody - Classic Poplar xylem PtrMYBTF1 primer pair
    H3pan monoclonal antibody - Classic
    H3K27me3 polyclonal antibody - Premium

  •  Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  •  Documents
    Universal Plant ChIP-seq kit MANUAL
    Manual of the Universal Plant ChIP-seq Kit
    Download
    Cell number estimation plants XLS FILE
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Universal Plant ChIP-seq kit (Diagenode Cat# C01010152). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Characterization of the Polycomb-Group Mark H3K27me3 in Unicellular Algae
    Mikulski P. et al.
    Polycomb Group (PcG) proteins mediate chromatin repression in plants and animals by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and ...

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    Oct 16-Oct 20, 2018
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