Diagenode

ChIPmentation Kit for Histones

カタログ番号
フォーマット
価格
C01011009
24 rxns
$1,995.00

ChIPmentation is a method that combines chromatin immunoprecipiation and tagmentation-based library preparation using a fast and robust ChIP-seq protocol for studying protein/DNA interactions. In this method, following chromatin immunoprecipitation, the sequencing libraries are created directly on the chromatin-antibody-beads complex by the Tagmentase (Tn5 transposase) loaded with sequencing adapters.  

The ChIPmentation Kit for Histones includes all reagents for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation.  The primer indexes for multiplexing are not included in the kit and have to be purchase separately:

Benefits of the ChIPmentation system for histone ChIP-seq

  • Easier and faster than classical ChIP-seq
  • Validated for various histone marks for a standard amount of cells
  • Generate high quality sequencing data

For low input samples (10,000 cells) we recommend the µChIPmentation kit for Histones.

For ChIP-seq on transcription factors we recommend the iDeal ChIP-seq for transcription factors + TAG kit for ChIPmentation

  • Validation

    The Diagenode ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.

    Figure 1: ChIPmentation sequencing results obtained from decreasing starting amounts of cells.

    Chromatin preparation has been performed on 7 M  K562 cells using the ChIPmentation Kit for Histones (Cat. no. C01011009) and 24 SI for ChIPmentation  (Cat. No. C01011031). Diluted chromatin from 100.000, 10.000 and 5.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003). A. Distribution of the ChIPmentation readsets in a representative region of the genome. B., C. and D. Comparison of the top 40% peaks from 100.000 (B.), 10,000 (C.) and 5.000 (D.) cells with ENCODE dataset.

    Figure 2: ChIPmentation sequencing results.

    Chromatin preparation has been performed on 7 M HeLa cells using the ChIPmentation Kit for Histones and 24 SI for ChIPmentation. Diluted chromatin from 100.000 cells was used for the immunoprecipitation with the Diagenode antibody targeting H3K4me3 (Cat. no. C15410003) and H3K27me3 (Cat. no. C15410195) and IgG (Cat. no. C15410206).

  • Additional solutions compatible with ChIPmentation Kit for Histones

    Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones) optimizes chromatin shearing, a critical step for ChIP.

    ChIP-seq grade anti-histone antibodies provide high yields with excellent specificity and sensitivity.

    For fast and efficient isolation of magnetic beads we recommend the magnetic racks DiaMag1.5 and DiaMag0.2.

    Primer indexes for tagmenteted libraries:

    The kit ChIPmentation for Histones is validated on the IP-Star Compact System and the corresponding protocol is included in the manual.

  •  資料
    ChIPmentation for Histones MANUAL
    Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of lib...
    Download
  •  Safety sheets
    ChIPmentation Kit for Histones SDS GB en Download
    ChIPmentation Kit for Histones SDS US en Download
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ChIPmentation Kit for Histones (Diagenode Cat# C01011009). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia
    L. Tottone at al.
    Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell leukemia (T-ALL). Here, we identify a highly conserved distal enh...

    Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells
    Tryndyak, V.P., Borowa-Mazgaj, B., Steward, C.R. et al. Epigenetic effects of low-level sodium arsenite exposure on human liver HepaRG cells.
    Chronic exposure to inorganic arsenic is associated with a variety of adverse health effects, including lung, bladder, kidney, and liver cancer. Several mechanisms have been proposed for arsenic-induced tumorigenesis; however, insufficient knowledge and many unanswered questions remain to explain the integrated mole...

    Distinct and temporary-restricted epigenetic mechanisms regulate human αβ and γδ T cell development
    Roels J, Kuchmiy A, De Decker M, et al.
    The development of TCRαβ and TCRγδ T cells comprises a step-wise process in which regulatory events control differentiation and lineage outcome. To clarify these mechanisms, we employed RNA-sequencing, ATAC-sequencing and ChIPmentation on well-defined thymocyte subsets that represent the conti...

    Measuring Histone Modifications in the Human Parasite Schistosoma mansoni
    de Carvalho Augusto R, Roquis D, Al Picard M, Chaparro C, Cosseau C, Grunau C.
    DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by sequencing (ChIP-seq) are useful to characterize the association of ge...

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    Diagenode
    Dec 8, 2021
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