Diagenode

Auto MagMeDIP qPCR Kit

Catalog Number
Format
Price
C02010014
48 rxns
$615.00
Other format

Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methylation status of your sample using 5-methylcytosine antibody. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.

Diagenode’s Auto MagMeDIP qPCR is available in two formats (10 and 48 IPs) and has been optimized on the SX-8G IP-Star® Automated System enabling highly reproducible results and allowing for high throughput assays.

Characteristics

  • Generate highly consistent results with internal controls in 24h
  • Minimize error with many reagents in 1 tube
  • Optimized purification (DIB - DNA isolation buffer)
  • Allows direct correlation between IP’d material & methylation status

MagMeDIP kit validated using Bioruptor

Figure 1. IP results obtained with Diagenode Auto MagMeDIP qPCR Kit. MeDIP assays were performed manually using DNA from blood, Gm12878, Hela and U20S cells and the Auto MagMeDIP qPCR kit (Diagenode). The DNA was prepared with the XL GenDNA Extraction Module included. The IP was performed including the kit meDNA and unDNA spike-in controls, together with the human DNA sample controls. The DNA was isolated/purified using DIB. Afterwards, qPCR was performed using the primer pairs also included in this kit.

Figure 2. Automated MeDIP (9h). IP reaction was performed on the SX-8G IP-Star® Automated System with the anti-5-mC antibody. Methylated and unmethylated DNA were used as internal controls. Unmethylated DNA region of GADPH and a methylated DNA region of AlphaX1 were used to test DNA sample-IP efficiency. DNA has been isolated by using DNA Isolation Buffer (DIB).

  •  Applications
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP i... Read more
  •  Documents
    Automating Whole-Genome DNA Methylation Analyses POSTER
    A step toward identifying subtle epigenetic variation in cancer and other complex disease
    Download
    Auto MagMeDIP kit MANUAL
    The Diagenode Auto MagMeDIP kit is designed to perform automated immunoprecipitation of methylate...
    Download
    Auto MeDIP kit MANUAL
    The Diagenode Auto MagMeDIP Kit (previous version named Auto MeDIP Kit) is designed to perf...
    Download
    MagMeDIP qPCR Kit MANUAL
    The Diagenode MagMeDIP kit is designed to immunoprecipitate methylated DNA (Methyl DNA IP...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Auto MagMeDIP qPCR Kit (Diagenode Cat# C02010014). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Protocols for Genetic and Epigenetic Studies of Rare Diseases Affecting Dental Tissues.
    Amorim BR, Dos Santos PAC, de Lima CL, Andia DC, Mazzeu JF, Acevedo AC
    This chapter describes methods related to the diagnosis of genetic dental diseases. Based on the present knowledge, clinical phenotyping and next-generation sequencing techniques are discussed. Methods necessary for Sanger sequencing, multiplex ligation-dependent probe amplification, and epigenetic modification meth...

    Molecular Signatures of Regression of the Canine Transmissible Venereal Tumor.
    Frampton D, Schwenzer H, Marino G, Butcher LM, Pollara G, Kriston-Vizi J, Venturini C, Austin R, de Castro KF, Ketteler R, Chain B, Goldstein RA, Weiss RA, Beck S, Fassati A
    The canine transmissible venereal tumor (CTVT) is a clonally transmissible cancer that regresses spontaneously or after treatment with vincristine, but we know little about the regression mechanisms. We performed global transcriptional, methylation, and functional pathway analyses on serial biopsies of vincristine-t...

    Saliva as a Blood Alternative for Genome-Wide DNA Methylation Profiling by Methylated DNA Immunoprecipitation (MeDIP) Sequencing
    Staunstrup N.H. et al.
    Background: Interrogation of DNA methylation profiles hold promise for improved diagnostics, as well as the delineation of the aetiology for common human diseases. However, as the primary tissue of the disease is often inaccessible without complicated and inconvenient interventions, there is an increasing interest i...

    Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples
    Bak S.T. et al.
    We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), co...

    Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
    Nicklas H. Staunstrup et al.
    Background In utero and early-life experienced environmental exposures are suggested to play an important role in many multifactorial diseases potentially mediated through lasting effects on the epigenome. As the epigenome in addition remains modifiable throughout life, identifying specific disease-relevant biomarke...

    Genome-wide non-CpG methylation of the host genome during M. tuberculosis infection
    Sharma G et al.
    A mammalian cell utilizes DNA methylation to modulate gene expression in response to environmental changes during development and differentiation. Aberrant DNA methylation changes as a correlate to diseased states like cancer, neurodegenerative conditions and cardiovascular diseases have been documented. Here we sho...

  •  Related products

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics