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Auto hMeDIP kit x16 (monoclonal mouse antibody)

Catalog Number
Format
Price
C02010034
16 rxns
$535.00

The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features a highly specific monoclonal antibody against 5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA. It includes control DNA and primers to assess the effiency of the assay. Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.

  • Characteristics
    • Robust enrichment & immunoprecipitation of hydroxymethylated DNA
    • Highly specific monoclonal antibody against 5-hmC for reliable, reproducible results
    • Including control DNA and primers to monitor the efficiency of the assay
      • 5-hmC, 5-mC and unmethylated DNA sequences and primer pairs
      • Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse
    • Improved single-tube, magnetic bead-based protocol
  •  Documents
    Auto hMeDIP kit MANUAL
    The Auto hMeDIP kits allow you to perform DNA hydroxymethylation analysis of your sample to...
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    DNA Methylation Solutions BROCHURE
    Complete solution for DNA methylation studies! Whether you are experienced or new to th...
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    Exclusive Highly Specific Kits Antibodies for DNA HydroxyMethylation Studies POSTER
    Cytosine hydroxymethylation was recently discovered as an important epigenetic mechanism. This cy...
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  •  Publications

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    Involvement of Epigenetic Modifications of GABAergic Interneurons in Basolateral Amygdala in Anxiety-like Phenotype of Prenatally Stressed Mice.
    Zhu C, Liang M, Li Y, Feng X, Hong J, Zhou R
    Background: Prenatal stress is considered a risk factor for anxiety disorder. Downregulation in the expression of GABAergic gene, that is, glutamic acid decarboxylase 67, associated with DNA methyltransferase overexpression in GABAergic neurons has been regarded as a characteristic component of anxiety disorder. Pre...

    TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors
    Ancey P.B. et al.
    Understanding the processes that govern liver progenitor cell differentiation has important implications for the design of strategies targeting chronic liver diseases, whereby regeneration of liver tissue is critical. Although DNA methylation (5mC) and hydroxymethylation (5hmC) are highly dynamic during early embryo...

    Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato
    Roldan M.V.G. et al.
    Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j) and jointless-2 (j-2), con...

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