This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Systems. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the efficiency of your immunoprecipitation.
Diagenode strongly recommends using this: Auto hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010034). Click here to copy to clipboard.
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Epigenetic remodelling of enhancers in response to estrogen deprivationand re-stimulation. Sklias Athena et al. Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating estrogen signaling, orchestrates a complex molecular circuitry that is not yet fully elucidated. Here, we investigated genome-wide DNA methylation, histone acetylation and transcriptio...
Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato Roldan M.V.G. et al. Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j) and jointless-2 (j-2), con...
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