hMeDIP kit x16 (monoclonal mouse antibody)

16 rxns

This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcytosine (5-hmC )(20µl, 50µl, 100µl) available in the market. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the effiency of your immunoprecipitation.

  • Characteristics
    • Robust enrichment & immunoprecipitation of hydroxymethylated DNA
    • Highly specific monoclonal antibody against 5-hmC for reliable, reproducible results
    • Including control DNA and primers to monitor the efficiency of the assay
      • 5-hmC, 5-mC and unmethylated DNA sequences and primer pairs
      • Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse
    • Improved single-tube, magnetic bead-based protocol
  •  文档
    hMeDIP kit MANUAL
    The Diagenode hMeDIP kit is designed to immunoprecipitate hydroxymethylated DNA (hMethyl ...
    Exclusive Highly Specific Kits Antibodies for DNA HydroxyMethylation Studies POSTER
    Cytosine hydroxymethylation was recently discovered as an important epigenetic mechanism. This cy...
    DNA Methylation Solutions BROCHURE
    Complete solution for DNA methylation studies! Whether you are experienced or new to th...
  •  Safety sheets
    hMeDIP kit x16 monoclonal mouse antibody SDS GB en Download
    hMeDIP kit x16 monoclonal mouse antibody SDS US en Download
    hMeDIP kit x16 monoclonal mouse antibody SDS BE nl Download
    hMeDIP kit x16 monoclonal mouse antibody SDS BE fr Download
    hMeDIP kit x16 monoclonal mouse antibody SDS FR fr Download
    hMeDIP kit x16 monoclonal mouse antibody SDS ES es Download
    hMeDIP kit x16 monoclonal mouse antibody SDS DE de Download
    hMeDIP kit x16 monoclonal mouse antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Developmental and Injury-induced Changes in DNA Methylation inRegenerative versus Non-regenerative Regions of the VertebrateCentral Nervous System
    Reverdatto S. et al.
    Background Because some of its CNS neurons (e.g., retinal ganglion cells after optic nerve crush (ONC)) regenerate axons throughout life, whereas others (e.g., hindbrain neurons after spinal cord injury (SCI)) lose this capacity as tadpoles metamorphose into frogs, the South African claw-toed frog, Xenopus laevis, o...

    Bone marrow age dictates clonality of smooth muscle-derived cells in theatherosclerotic plaque
    Kabir Inamul et al.
    Aging is the predominant risk factor for atherosclerosis, the leading cause of death. Rare smooth muscle cells (SMCs) clonally expand giving rise to up to ∼70\% of atherosclerotic plaque cells; however, the effect of age on SMC clonality is not known. Our results indicate that aging induces SMC polyclonality and...

    Epigenetic remodelling of enhancers in response to estrogen deprivationand re-stimulation.
    Sklias Athena et al.
    Estrogen hormones are implicated in a majority of breast cancers and estrogen receptor alpha (ER), the main nuclear factor mediating estrogen signaling, orchestrates a complex molecular circuitry that is not yet fully elucidated. Here, we investigated genome-wide DNA methylation, histone acetylation and transcriptio...

    Transcription of synaptic plasticity-related genes in patients with somnipathy combined with type 2 diabetes
    Yi Zhang, Rui Ma, Shaohong Zou, Gaiyu Tong, Gulibakeranmu Abula, Manna Hu, Qing Dai
    Objective: To investigate DNA methylation and hydroxymethylation in patients with somnipathy combined with type 2 diabetes, and to explore the effects of DNA methylation and hydroxymethylation on gene expression. Methods: Thirty patients with somnipathy combined with type 2 diabetes and 20 patients with type-2 diab...

    Emerging Role of One-Carbon Metabolism and DNA Methylation Enrichment on δ-Containing GABAA Receptor Expression in the Cerebellum of Subjects with Alcohol Use Disorders (AUD
    Gatta E. et al.
    Background Cerebellum is an area of the brain particularly sensitive to the effects of acute and chronic alcohol consumption. Alcohol exposure decreases cerebellar Purkinje cell output by increasing GABA release from Golgi cells onto extrasynaptic α6/δ-containing GABAA receptors located on glutamate...

    Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus
    Lu F. et al.
    Epstein-Barr Virus (EBV) latency and its associated carcinogenesis are regulated by dynamic changes in DNA methylation of both virus and host genomes. We show here that the Ten-Eleven Translocation 2 (TET2) gene, implicated in hydroxymethylation and active DNA demethylation, is a key regulator of EBV latency type DN...

    Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer
    Vafadar-Isfahani N. et al.
    Hypomethylation of LINE-1 repeats in cancer has been proposed as the main mechanism behind their activation; this assumption, however, was based on findings from early studies that were biased toward young and transpositionally active elements. Here, we investigate the relationship between methylation of 2 intergeni...

    Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex
    Labouesse MA et al.
    Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...

    Hepatic DNA hydroxymethylation is site-specifically altered by chronic alcohol consumption and aging
    Tammen SA et al.
    PURPOSE: Global DNA hydroxymethylation is markedly decreased in human cancers, including hepatocellular carcinoma, which is associated with chronic alcohol consumption and aging. Because gene-specific changes in hydroxymethylcytosine may affect gene transcription, giving rise to a carcinogenic environment, we det...

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