|Datasheet DNA hydroxymethylation control package DATASHEET||Download|
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be added directly to the DNA sample of interest before any hydroxymethylation profiling experiment (e.g. with Diagenode's hMeDIP Kit).
The hydroxymethylated spike-in control is a linear dsDNA that has been produced by PCR using the Diagenode’s MethylTaq DNA polymerase and hydroxymethyl dCTPs replacing normal cytosine nucleotides.
The hydroxymethylated spike-in control is based on a synthetic sequence and does not show significant homology with model species (or human, mouse or rat genomic sequences).