Diagenode

hMeDIP kit x16 (monoclonal mouse antibody)

カタログ番号
フォーマット
価格
C02010031
(AF-110-0016)
16 rxns
$510.00

このキットは、5-ヒドロキシメチルシトシン(5-hmC)(20µl, 50µl, 100µl)に対する最初で唯一のモノクローナル抗体を用いたDNA免疫沈降実験専用に設計されています。キットには、免疫沈降の効率を評価するコントロール配列(5-hmC、5-mCおよび未修飾シトシンDNAスタンダード)とコントロールプライマーペア(内部および外部コントロール)が含まれています。

  • 特徴
    • Immunoprecipiation performed with monoclonal antibody against 5-hmC
    • Including internal & external controls for QC of each reaction
      • Mouse primers against Sf1 (hydroxymethylated gene in mouse)
      • 5-hmC, 5-mC and unmethylated DNA
    • Complete magnetic bead based kit
    • All in one tube
  •  実験手法
    メチル化DNA免疫沈降
    hMeDIPの場合、メチル化DNA IP(MeDIP)は5-メチルシトシン(5-mC)または5-ヒドロキシメチルシトシン(5-hmC)に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 使い方 メチルDNA IPは以下のようにして実施されます。(要約):培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、... Read more
    エピジェネティクス・DNAメチル化
    DNAメチル化研究のための完全打開策 Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。 DNAメチル化は最初に発見されたエピジェネティクスマークであり、エピジェネティクスス... Read more
  •  資料
    hMeDIP kit MANUAL
    The Diagenode hMeDIP kit is designed to immunoprecipitate hydroxymethylated DNA (hMethyl ...
    Download
    Exclusive Highly Specific Kits Antibodies for DNA HydroxyMethylation Studies POSTER
    5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA by two US groups (K...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Transcription of synaptic plasticity-related genes in patients with somnipathy combined with type 2 diabetes
    Yi Zhang, Rui Ma, Shaohong Zou, Gaiyu Tong, Gulibakeranmu Abula, Manna Hu, Qing Dai
    Objective: To investigate DNA methylation and hydroxymethylation in patients with somnipathy combined with type 2 diabetes, and to explore the effects of DNA methylation and hydroxymethylation on gene expression. Methods: Thirty patients with somnipathy combined with type 2 diabetes and 20 patients with type-2 diab...

    Emerging Role of One-Carbon Metabolism and DNA Methylation Enrichment on δ-Containing GABAA Receptor Expression in the Cerebellum of Subjects with Alcohol Use Disorders (AUD
    Gatta E. et al.
    Background Cerebellum is an area of the brain particularly sensitive to the effects of acute and chronic alcohol consumption. Alcohol exposure decreases cerebellar Purkinje cell output by increasing GABA release from Golgi cells onto extrasynaptic α6/δ-containing GABAA receptors located on glutamate...

    Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus
    Lu F. et al.
    Epstein-Barr Virus (EBV) latency and its associated carcinogenesis are regulated by dynamic changes in DNA methylation of both virus and host genomes. We show here that the Ten-Eleven Translocation 2 (TET2) gene, implicated in hydroxymethylation and active DNA demethylation, is a key regulator of EBV latency type DN...

    Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer
    Vafadar-Isfahani N. et al.
    Hypomethylation of LINE-1 repeats in cancer has been proposed as the main mechanism behind their activation; this assumption, however, was based on findings from early studies that were biased toward young and transpositionally active elements. Here, we investigate the relationship between methylation of 2 intergeni...

    Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex
    Labouesse MA et al.
    Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...

    Hepatic DNA hydroxymethylation is site-specifically altered by chronic alcohol consumption and aging
    Tammen SA et al.
    PURPOSE: Global DNA hydroxymethylation is markedly decreased in human cancers, including hepatocellular carcinoma, which is associated with chronic alcohol consumption and aging. Because gene-specific changes in hydroxymethylcytosine may affect gene transcription, giving rise to a carcinogenic environment, we det...

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