Diagenode

Bisulfite conversion reagent for RRBS

カタログ番号
フォーマット
価格
C02030035
8 rxns
$99.00

This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.

Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a cost-effective method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.

  •  実験手法
    バイサルファイト処理
    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylate... Read more
    エピジェネティクス・DNAメチル化
    DNAメチル化研究のための完全打開策 Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。 DNAメチル化は最初に発見されたエピジェネティクスマークであり、エピジェネティクスス... Read more
  •  資料
    Diagenode Premium RRBS technology: Cost-effective DNA methylation mapping with superior CpG resolution and coverage APPLICATION NOTE
    DNA methylation is an important epigenetic mark with broad relevance in development and disease. ...
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    Premium RRBS kit MANUAL
    Diagenode’s Premium RRBS technology Positive and negative spike-in controls are...
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    Bisulfite conversion reagent for RRBS Datasheet DATASHEET
    This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfi...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Bisulfite conversion reagent for RRBS (Diagenode Cat# C02030035). Click here to copy to clipboard.

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    Diagenode® Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage
    Anne-Clémence Veillard, Paul Datlinger, Miklos Laczik, Sharon Squazzo & Christoph Bock
    Reduced representation bisulfite sequencing (RRBS) enables genome-scale DNA methylation analysis in any vertebrate species. The assay benefits from the practical advantages of bisulfite sequencing while avoiding the cost of whole-genome sequencing. The Diagenode Premium RRBS kit makes this technology widely availabl...

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