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MagMeDIP-seq Package

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Catalog Number
Format
C02010040
10 rxns
DISCONTINUED

Discover our NEW MagMeDIP-seq Package V2.

An updated version of this package is available for superior performance with lower DNA amount.

Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.

This package contains everything you need from gDNA extraction to library preparation and quality check. It allows you to perform your NGS compatible MeDIP-seq assay, combining the strength of MagMeDIP technology with the power of our iDeal Library Preparation technique.

Features

  • Complete workflow from gDNA extraction to library preparation
  • Highly validated antibody (33D3)
  • Down to 100 ng DNA input
  • Generate highly consistent results with external & internal controls
  • Highest purification with IPure
  • Allows direct correlation between IP’d material & methylation status

  • Validation

    medip sequencing coverage

    Figure 1. Consistent coverage and methylation detection from different starting amounts of DNA with the Diagenode MagMeDIP-seq Package. Samples containing decreasing starting amounts of DNA (from the top down: 1000 ng (red), 250 ng (blue), 100 ng (green)) originating from human blood were prepared, revealing a consistent coverage profile for the three different starting amounts, which enables reproducible methylation detection. The CpG islands (CGIs) (marked by yellow boxes in the bottom track) are predominantly unmethylated in the human genome, and as expected, we see a depletion of reads at and around CGIs.

    medip seq control regions

    Figure 2. Coverage profiles on the control regions of one of the human blood samples prepared with the Diagenode MagMeDIP-seq Package. The scales used for TSH2b (the positive control for methylation) and for the GAPDH (the negative control for methylation) are identical.
    (A) No methylation detected in the negative control region. The image shows the area around the TSS (Transcription Start Site) of the GAPDH gene. In line with our expectations for a housekeeping gene, we see an absence of peak in this region.
    (B) High methylation detected in the positive control region. The image shows the HIST1H2BA (TSH2b) gene and its surroundings. The HIST1H2BA gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene.

  •  Documents
    MagMeDIP-seq Package MANUAL
    Magnetic Methylated DNA Immunopreciptation Package for NGS. DNA methylation is a key epigenetic ...
    Download
    DNA Methylation Solutions BROCHURE
    Complete solution for DNA methylation studies! Whether you are experienced or new to th...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MagMeDIP-seq Package (Diagenode Cat# C02010040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Polycystic ovary syndrome is transmitted via a transgenerational epigenetic process
    Mimouni et. al.
    Polycystic ovary syndrome (PCOS) is the most common reproductive and metabolic disorder affecting women of reproductive age. PCOS has a strong heritable component, but its pathogenesis has been unclear. Here, we performed RNA sequencing and genome-wide DNA methylation profiling of ...

    Integrated epigenetic biomarkers in circulating cell-free DNA as a robust classifier for pancreatic cancer.
    Cao F, Wei A, Hu X, He Y, Zhang J, Xia L, Tu K, Yuan J, Guo Z, Liu H, Xie D, Li A
    BACKGROUND: The high lethal rate of pancreatic cancer is partly due to a lack of efficient biomarkers for screening and early diagnosis. We attempted to develop effective and noninvasive methods using 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) markers from circulating cell-free DNA (cfDNA) for the det...

    Detection of renal cell carcinoma using plasma and urine cell-free DNA methylomes.
    Nuzzo PV, Berchuck JE, Korthauer K, Spisak S, Nassar AH, Abou Alaiwi S, Chakravarthy A, Shen SY, Bakouny Z, Boccardo F, Steinharter J, Bouchard G, Curran CR, Pan W, Baca SC, Seo JH, Lee GM, Michaelson MD, Chang SL, Waikar SS, Sonpavde G, Irizarry RA, Pome
    Improving early cancer detection has the potential to substantially reduce cancer-related mortality. Cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) is a highly sensitive assay capable of detecting early-stage tumors. We report accurate classification of patients across all ...

    Detection and discrimination of intracranial tumors using plasma cell-free DNA methylomes.
    Nassiri F, Chakravarthy A, Feng S, Shen SY, Nejad R, Zuccato JA, Voisin MR, Patil V, Horbinski C, Aldape K, Zadeh G, De Carvalho DD
    Definitive diagnosis of intracranial tumors relies on tissue specimens obtained by invasive surgery. Noninvasive diagnostic approaches provide an opportunity to avoid surgery and mitigate unnecessary risk to patients. In the present study, we show that DNA-methylation profiles from plasma reveal highly specific sign...

    Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA.
    Shen SY, Burgener JM, Bratman SV, De Carvalho DD
    Circulating cell-free DNA (cfDNA) comprises small DNA fragments derived from normal and tumor tissue that are released into the bloodstream. Recently, methylation profiling of cfDNA as a liquid biopsy tool has been gaining prominence due to the presence of tissue-specific markers in cfDNA. We have previously reporte...

    DNA methylation of the Tacr2 gene in a CUMS model of depression.
    Xiang D, Xiao J, Fu L, Yao L, Wan Q, Xiao L, Zhu F, Wang G, Liu Z
    Tacr2, the gene encoding the NK2 receptor, belongs to G protein-coupled receptors. Accumulating evidence has indicated that the tachykinin receptors may contribute to the pathophysiology of depression. During the last decade, some studies have shown that Tacr2 activation is involved in the modulation of emotional pr...

    Sensitive tumour detection and classification using plasma cell-free DNA methylomes.
    Shen SY, Singhania R, Fehringer G, Chakravarthy A, Roehrl MHA, Chadwick D, Zuzarte PC, Borgida A, Wang TT, Li T, Kis O, Zhao Z, Spreafico A, Medina TDS, Wang Y, Roulois D, Ettayebi I, Chen Z, Chow S, Murphy T, Arruda A, O'Kane GM, Liu J, Mansour M, McPher
    The use of liquid biopsies for cancer detection and management is rapidly gaining prominence. Current methods for the detection of circulating tumour DNA involve sequencing somatic mutations using cell-free DNA, but the sensitivity of these methods may be low among patients with early-stage cancer given the limited ...

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Events

  • DNA methylation workshop: Become an expert at RRBS
    Diagenode
    Oct 1-Oct 31, 2021
  • Dutch Chromatin Meeting
    Radboud UMC, Geert Grooteplein 15, 6525 EZ Nijmegen, The Netherlands, Experience center, route 292 to 307
    Nov 12, 2021
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