Diagenode

MagMeDIP-seq Package

Catalog Number
Format
Price
C02010040
10 rxns
$1,299.00

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Sensitive tumour detection and classification using plasma cell-free DNA methylomes
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Perform MeDIP (Methylated DNA Immunoprecipitation) followed by sequencing to get DNA methylation status of your sample using 5-methylcytosine antibody and magnetic beads. Our kit contains high quality reagents to get the highest enrichment of methylated DNA with an optimized user-friendly protocol.

This package contains everything you need from gDNA extraction to library preparation and quality check. It allows you to perform your NGS compatible MeDIP-seq assay, combining the strength of MagMeDIP technology with the power of our iDeal Library Preparation technique.

Content

Features

  • Complete workflow from gDNA extraction to library preparation
  • Highly validated antibody (33D3)
  • Down to 100 ng DNA input
  • Generate highly consistent results with external & internal controls
  • Highest purification with IPure
  • Allows direct correlation between IP’d material & methylation status

  • Validation

    medip sequencing coverage

    Figure 1. Consistent coverage and methylation detection from different starting amounts of DNA with the Diagenode MagMeDIP-seq Package. Samples containing decreasing starting amounts of DNA (from the top down: 1000 ng (red), 250 ng (blue), 100 ng (green)) originating from human blood were prepared, revealing a consistent coverage profile for the three different starting amounts, which enables reproducible methylation detection. The CpG islands (CGIs) (marked by yellow boxes in the bottom track) are predominantly unmethylated in the human genome, and as expected, we see a depletion of reads at and around CGIs.

    medip seq control regions

    Figure 2. Coverage profiles on the control regions of one of the human blood samples prepared with the Diagenode MagMeDIP-seq Package. The scales used for TSH2b (the positive control for methylation) and for the GAPDH (the negative control for methylation) are identical.
    (A) No methylation detected in the negative control region. The image shows the area around the TSS (Transcription Start Site) of the GAPDH gene. In line with our expectations for a housekeeping gene, we see an absence of peak in this region.
    (B) High methylation detected in the positive control region. The image shows the HIST1H2BA (TSH2b) gene and its surroundings. The HIST1H2BA gene is coding for a histone variant that does not occur in blood cells, and it is known to be silenced by methylation. Accordingly, we see a high coverage in the vicinity of this gene.

  •  Applications
    DNA Methylation
    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data betwee... Read more
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP i... Read more
    Next Generation Sequencing
    DNA Shearing, library preparation, and automation: your one-stop shop for NGS 1. Choose your shearing device: Shear DNA anywhere from 150 bp to 75 kb Shear down to 5 μl: 150 bp - 2 kb Perfect for NGS DNA library prep and FFPE n... Read more
    DNA/RNA library preparation
    Most of the major next-generation sequencing platforms require ligation of specific adaptor oligos to fragmented DNA or RNA prior to sequencing After input DNA has been fragmented, it is end-repaired and blunt-ended. The next step is a A-tail... Read more
  •  Documents
    MagMeDIP-seq Package MANUAL
    Magnetic Methylated DNA Immunopreciptation Package for NGS. DNA methylation is a key epigenetic ...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MagMeDIP-seq Package (Diagenode Cat# C02010040). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    DNA methylation of the Tacr2 gene in a CUMS model of depression.
    Xiang D, Xiao J, Fu L, Yao L, Wan Q, Xiao L, Zhu F, Wang G, Liu Z
    Tacr2, the gene encoding the NK2 receptor, belongs to G protein-coupled receptors. Accumulating evidence has indicated that the tachykinin receptors may contribute to the pathophysiology of depression. During the last decade, some studies have shown that Tacr2 activation is involved in the modulation of emotional pr...

    Sensitive tumour detection and classification using plasma cell-free DNA methylomes.
    Shen SY, Singhania R, Fehringer G, Chakravarthy A, Roehrl MHA, Chadwick D, Zuzarte PC, Borgida A, Wang TT, Li T, Kis O, Zhao Z, Spreafico A, Medina TDS, Wang Y, Roulois D, Ettayebi I, Chen Z, Chow S, Murphy T, Arruda A, O'Kane GM, Liu J, Mansour M, McPher
    The use of liquid biopsies for cancer detection and management is rapidly gaining prominence. Current methods for the detection of circulating tumour DNA involve sequencing somatic mutations using cell-free DNA, but the sensitivity of these methods may be low among patients with early-stage cancer given the limited ...

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