Premium Whole Genome Bisulfite Sequencing (WGBS) kit

Catalog Number

Format

Price

C02030034

8 rxns

$835.00

Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes
Compatible with ChIP-Bis-Seq - Combining ChIP and DNA methylation profiles in one assay
Complete kit – Bisulfite conversion reagents and library preparation reagents

The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode’s Premium RRBS Kit.

Looking for multiplexing? Check out our Premium WGBS indexes

Characteristics

Diagenode_WGBS_RRBS_Comparison

Figure 1: Coverage of Diagenode’s Premium WGBS Kit (A) and Premium RRBS Kit (B).
WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit. RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage (A) of the whole genome using WGBS and (B) of the CpGs areas using RRBS.

ChIP-Bis-sequencing results

Figure 2: ChIP-Bis-sequencing results obtained with the Diagenode’s Premium WGBS Kit
A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode’s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP’d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.

Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
	'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2648',
			'antibody_id' => null,
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
			'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030034',
			'old_catalog_number' => '',
			'sf_code' => 'C02030034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '815',
			'price_USD' => '835',
			'price_GBP' => '745',
			'price_JPY' => '142400',
			'price_CNY' => '',
			'price_AUD' => '2090',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-kit',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-05-27 15:36:29',
			'created' => '2015-07-09 15:03:28',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			),
			(int) 8 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine'
$meta_description = 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.'
$meta_title = 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2648',
		'antibody_id' => null,
		'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
		'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '8 rxns',
		'catalog_number' => 'C02030034',
		'old_catalog_number' => '',
		'sf_code' => 'C02030034-',
		'type' => 'RFR',
		'search_order' => '04-undefined',
		'price_EUR' => '815',
		'price_USD' => '835',
		'price_GBP' => '745',
		'price_JPY' => '142400',
		'price_CNY' => '',
		'price_AUD' => '2090',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => true,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'premium-wgbs-kit',
		'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
		'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
		'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
		'modified' => '2020-05-27 15:36:29',
		'created' => '2015-07-09 15:03:28',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1892',
			'antibody_id' => null,
			'name' => 'Premium Bisulfite kit',
			'description' => '<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C02030030',
			'old_catalog_number' => '',
			'sf_code' => 'C02030030-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-bisulfite-kit-50-rxns',
			'meta_title' => 'Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Premium Bisulfite kit',
			'modified' => '2021-12-08 09:03:59',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2826',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 6',
			'description' => '<p style="text-align: justify;"><span>Theses 6 different indexes are designed for the <a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span><span></span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combines ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C05010032',
			'old_catalog_number' => '',
			'sf_code' => 'C05010032-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '740',
			'price_GBP' => '655',
			'price_JPY' => '129500',
			'price_CNY' => '',
			'price_AUD' => '1850',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-6',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 48 rxns | Diagenode',
			'meta_keywords' => '6 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 6 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:18',
			'created' => '2016-08-18 11:41:52',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 5 => array(
			'id' => '2827',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 12',
			'description' => '<p style="text-align: justify;"><span>Theses 12 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '96 rxns',
			'catalog_number' => 'C05010033',
			'old_catalog_number' => '',
			'sf_code' => 'C05010033-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '1260',
			'price_USD' => '1260',
			'price_GBP' => '1085',
			'price_JPY' => '220100',
			'price_CNY' => '',
			'price_AUD' => '3150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-12',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 96 rxns | Diagenode',
			'meta_keywords' => '12 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 12 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:02',
			'created' => '2016-08-18 11:49:42',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 6 => array(
			'id' => '2828',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 24',
			'description' => '<p style="text-align: justify;"><span>Theses 24 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '192 rxns',
			'catalog_number' => 'C05010034',
			'old_catalog_number' => '',
			'sf_code' => 'C05010034-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '2265',
			'price_USD' => '2265',
			'price_GBP' => '2050',
			'price_JPY' => '394300',
			'price_CNY' => '',
			'price_AUD' => '5665',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-24',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 192 rxns | Diagenode',
			'meta_keywords' => '24 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 24 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:34',
			'created' => '2016-08-18 11:49:49',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 7 => array(
			'id' => '2836',
			'antibody_id' => null,
			'name' => 'RRBS受託サービス (Reduced Representation Bisulfite Sequencing)',
			'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p>
<div class="extra-spaced">
<p>メチル化アレイとWGBS技術の長所を有するReduced representation bisulfite sequencing (RRBS) 技術は、あらゆる脊椎動物種で柔軟に使用できるため、大規模コホートの研究においても正確で費用対効果の高いアッセイを行えます。制限MspI酵素（CCGGターゲットサイト）を使用してゲノムを切断し、次にサイズを選択することで、濃縮されたDNAはプロモーターやCpGアイランドを含む関連するターゲットCpGリッチ領域を表します。またRRBS技術はゲノムの一部分を濃縮してメチル化解析を行うことによってシーケンス量を減らす手法でカバレッジが非常に高く、CpGメチル化解析に適しています。（ヒトサンプルで最大700万CpGが検出）。</p>
<h2>Genome-scale DNA methylation analysis</h2>
<ul class="square">
<li>Robust and cost-effective solution with&nbsp;reliable results</li>
<li>High coverage - up to 7 million CpGs detected in human samples</li>
<li>Single nucleotide resolution</li>
<li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li>
<li>Suitable for&nbsp;any vertebrate species</li>
</ul>
<h2>Complete end-to-end service</h2>
<ul class="square">
<li>From&nbsp;DNA QC to standard bioinformatics&nbsp;</li>
<li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2&nbsp;</a>widely adopted</li>
<li>As little as 25 ng&nbsp;for&nbsp;any vertebrate species</li>
<li>Differentially methylated site analysis&nbsp;</li>
</ul>
</div>
<div class="extra-spaced">
<h2></h2>
</div>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label1' => '詳細',
			'info1' => '<div class="row">
<div class="small-6 columns">
<h4>RRBS Service includes:</h4>
<table style="width: 905px;">
<tbody>
<tr>
<td style="width: 264px;"><strong>QC of the genomic DNA</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Measurement of DNA concentration&nbsp;</li>
<li>Assessment of DNA quality</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>MspI digestion</li>
<li>Library preparation (ends preparation, adaptor ligation)</li>
<li>Size selection</li>
<li>Sample pooling</li>
<li>Bisulfite conversion</li>
<li>Library amplification and clean-up</li>
<li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Deep sequencing</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li>
<li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li>
<li>7&nbsp;million CpGs (on average) for human samples</li>
<li>7-11x CpG coverage&nbsp;(on average) for human samples</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'バイオインフォマティック解析',
			'info2' => '<div class="row">
<div class="small-6 columns">
<table style="width: 895px;">
<thead>
<tr>
<th style="width: 262px;">
<h4><strong>Analysis</strong></h4>
</th>
<th style="width: 624px;">
<h4><strong>Features</strong></h4>
</th>
</tr>
</thead>
<tbody>
<tr>
<td style="width: 262px;"><strong>Standard</strong></td>
<td style="width: 624px;">
<ul>
<li>FASTQ raw data</li>
<li>FASTQC quality control insights</li>
<li>Alignment of bisulfite sequencing data against reference genome</li>
<li>Methylation calling and extraction</li>
<li>Summary statistics</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;"><strong>Differential&nbsp;methylation analysis<br /></strong></td>
<td style="width: 624px;">
<ul>
<li>Methylation level analysis</li>
<li>Differentially Methylated CpGs (DMCs) analysis</li>
<li>Differentially Methylated Regions (DMRs) analysis</li>
<li>Annotation of DMCs and DMRs for genomic regions (exons, introns, &hellip;)</li>
<li>Clustering analysis</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Gene ontology terms analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Enrichment analysis on gene associated with DMCs and DMRs</li>
<li>Get functional insights</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Pathway analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li>
<li>Get mechanistic insights</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '',
			'catalog_number' => 'G02020000',
			'old_catalog_number' => '',
			'sf_code' => '',
			'type' => 'ACC',
			'search_order' => '',
			'price_EUR' => '/',
			'price_USD' => '/',
			'price_GBP' => '/',
			'price_JPY' => '42800',
			'price_CNY' => '/',
			'price_AUD' => '/',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => true,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rrbs-service',
			'meta_title' => 'Reduced Representation Bisulfite Sequencing（RRBS）サービス| Diagenode',
			'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation',
			'meta_description' => 'Diagenode社のプレミアムRRBS（還元重亜硫酸塩シークエンシング）キットは、ほぼ90％のアライメント率、410万CpGのカバー率、重亜硫酸塩転換率はすべてのサンプルで約99.5％',
			'modified' => '2022-04-08 08:31:12',
			'created' => '2016-09-21 16:54:44',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 8 => array(
			'id' => '2853',
			'antibody_id' => null,
			'name' => 'Bisulfite conversion reagent for RRBS',
			'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030035',
			'old_catalog_number' => '',
			'sf_code' => 'C02030035-',
			'type' => 'REF',
			'search_order' => '01-undefined',
			'price_EUR' => '115',
			'price_USD' => '115',
			'price_GBP' => '110',
			'price_JPY' => '20900',
			'price_CNY' => '',
			'price_AUD' => '290',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bisulfite-conversion-package-for-RRBS',
			'meta_title' => 'Bisulfite conversion package for RRBS',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
			'modified' => '2019-12-16 10:57:53',
			'created' => '2016-11-18 23:22:25',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '6',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'メチル化DNA結合タンパク質',
			'description' => '<div class="row">
<div class="large-12 columns">MBD方法は、メチル化DNAに対するH6-GST-MBD融合タンパク質の非常に高い親和性に基づいています。 このタンパク質は、N末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）を含有します。 このH6-GST-MBD融合タンパク質を用いて、メチル化CpGを含むDNAを特異的に単離する事が可能です。<br /><br />DiagenodeのMethylCap&reg;キットは、二本鎖DNAの高濃縮と、メチル化CpG密度の関数における微分分画を可能にします。 分画はサンプルの複雑さを軽減し、次世代のシーケンシングを容易にします。 MethylCapアッセイに先立ち、DNAを最初に抽出し、Picoruptorソニケーターを用いて断片化します。<br />
<h3>概要</h3>
<p class="text-center"><br /><img src="https://www.diagenode.com/img/applications/methyl_binding_domain_overview.jpg" /></p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'slug' => 'methylbinding-domain-protein',
			'meta_keywords' => 'Epigenetic,Methylbinding Domain Protein,MBD,DNA methylation,DNA replication,MethylCap,MethylCap assay,',
			'meta_description' => 'Methylbinding Domain Protein(MBD) approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase',
			'meta_title' => 'Epigenetic Methylbinding Domain Protein(MBD) - DNA methylation | Diagenode',
			'modified' => '2019-03-22 12:32:12',
			'created' => '2015-06-02 17:05:42',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<p><br />Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Whole Genome Bisulfite Sequencing (WGBS) with our <a href="https://www.diagenode.com/en/p/premium-wgbs-kit">Premium WGBS Kit</a></li>
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2022-12-02 11:57:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '790',
			'name' => 'Premium WGBS kit',
			'description' => '<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<p><span>Whole Genome Bisulfite Sequencing </span><span>(WGBS) is a single nucleotide resolution technique that allows the user to study DNA methylation sites and their role in gene regulation. The Diagenode&rsquo;s </span><span>Premium WGBS Kit </span><span>is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for the preparation of bisulfite converted library from ChIP&rsquo;d samples in order to perform </span><span>ChIP-Bis-Sequencing</span><span>. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s Premium RRBS Kit (Cat. No.C02030032 and C02030033). </span></p>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Premium_WGBS_manual.pdf',
			'slug' => 'premium-wgbs-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:19:28',
			'created' => '2015-07-09 15:56:04',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '36',
			'name' => 'DNA Methylation Solutions',
			'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Brochure',
			'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
			'slug' => 'dna-methylation-brochure',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 11:21:31',
			'created' => '2015-07-03 16:05:27',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3742',
			'name' => 'Development and epigenetic plasticity of murine Müller glia.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of M&uuml;ller glia. To understand the role of mammalian M&uuml;ller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expression during differentiation of retinal progenitor cells (RPCs) into M&uuml;ller glia. We also analyzed the global epigenetic profile of adult M&uuml;ller glia. We observed significant changes in gene expression during differentiation of RPCs into M&uuml;ller glia in only a small group of genes. We found a high similarity between RPCs and M&uuml;ller glia on the transcriptomic and epigenomic levels. Our findings also indicate that M&uuml;ller glia are epigenetically very close to late-born retinal neurons, but not early-born retinal neurons. Importantly, we found that key genes required for phototransduction were highly methylated. Thus, our data suggest that M&uuml;ller glia are epigenetically very similar to late RPCs. Meanwhile, obstacles for regeneration of the entire mammalian retina from M&uuml;ller glia may consist of repressive chromatin and highly methylated DNA in the promoter regions of many genes required for the development of early-born retinal neurons. In addition, DNA demethylation may be required for proper reprogramming and differentiation of M&uuml;ller glia into rod photoreceptors.</p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>',
			'date' => '2019-07-02',
			'pmid' => 'http://www.pubmed.gov/31276697',
			'doi' => '10.1016/j.bbamcr.2019.06.019',
			'modified' => '2019-08-13 10:50:24',
			'created' => '2019-07-31 13:35:50',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3569',
			'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
			'date' => '2019-03-07',
			'pmid' => 'http://www.pubmed.gov/30846751',
			'doi' => '10.1038/s41598-019-40262-w',
			'modified' => '2019-05-09 17:33:09',
			'created' => '2019-03-21 14:12:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2422',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2424',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2418',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2417',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2421',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2420',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2419',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2423',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030030</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1892" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-premium-bisulfite-kit-40-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030031</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1893" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1893" id="CartAdd/1893Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1893" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-premium-bisulfite-kit-40-rxns" data-reveal-id="cartModal-1893" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-6"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2826" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2826" id="CartAdd/2826Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2826" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 6</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                     '740',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                    '740',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-6" data-reveal-id="cartModal-2826" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 6</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-12"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2827" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2827" id="CartAdd/2827Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2827" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 12</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                     '1260',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                    '1260',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-12" data-reveal-id="cartModal-2827" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 12</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-24"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2828" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2828" id="CartAdd/2828Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2828" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 24</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                     '2265',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                    '2265',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-24" data-reveal-id="cartModal-2828" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 24</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/rrbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">G02020000</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2836" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

    </div>
</div>

<form action="/en/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RWM1TjFTVlNVWUlrQjBQOHRxaFRWUT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
    <div class="row collapse">
        <h2>Service Information</h2>
    </div>

    <div class="small-12 large-12 columns">
        <h4>Which services are you interested in?</h4>
    </div>
    <div class="small-12 large-12 columns">
        <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/>

<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium EPIC Array" id="QuoteEpigenomicsServiceInfiniumEPICArray" /><label for="QuoteEpigenomicsServiceInfiniumEPICArray">Infinium EPIC Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div>
    </div>


    <div class="row collapse">
        <div class="small-12 medium-12 large-3 columns">
            <span class="prefix">Sample species</span>
        </div>
        <div class="small-12 medium-12 large-9 columns">
            <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/>        </div>
    </div>

    <div class="row collapse">
        <div class="small-12 medium-12 large-6 columns">
            <span class="prefix">Total number of samples (including replicates)</span>
        </div>
        <div class="small-12 medium-12 large-6 columns">
            <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/>        </div>
    </div>


<div class="row collapse">
    <h2>Contact Information</h2>
    <div class="small-3 large-2 columns">
        <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-2836').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-2836" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Project</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Describe your project" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha642430804f083"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-2836" class="alert button expand" form="Quote-2836" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-2836').attr('action','javascript:postToPardot(\'' + $('#Quote-2836').attr('action') + '\', 2836)');
    });

    $("#Quote-2836 #submit_btn-2836").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-2836 #submit_btn-2836").attr("disabled", true);
            $("#Quote-2836 #submit_btn-2836").html("Processing...");

            //submit the form
            $("#Quote-2836").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'US') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'CA') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'DE') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else {
        $("#Quote-2836 #state-2836").parent().parent().hide();
        $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
    }

    $("#Quote-2836 #country_selector_quote-2836.selectized").change(function() {
        if (this.value == 'US') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'CA') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'DE') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else {
            $("#Quote-2836 #state-2836").parent().parent().hide();
            $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
        }

    });
</script>
<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="rrbs-service" data-reveal-id="quoteModal-2836" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">RRBS Service (Reduced Representation Bisulfite ...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/bisulfite-conversion-package-for-RRBS"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030035</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2853" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2853" id="CartAdd/2853Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2853" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Bisulfite conversion reagent for RRBS</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                     '115',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                    '115',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="bisulfite-conversion-package-for-RRBS" data-reveal-id="cartModal-2853" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Bisulfite conversion reagent for RRBS</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '2853',
	'antibody_id' => null,
	'name' => 'Bisulfite conversion reagent for RRBS',
	'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '8 rxns',
	'catalog_number' => 'C02030035',
	'old_catalog_number' => '',
	'sf_code' => 'C02030035-',
	'type' => 'REF',
	'search_order' => '01-undefined',
	'price_EUR' => '115',
	'price_USD' => '115',
	'price_GBP' => '110',
	'price_JPY' => '20900',
	'price_CNY' => '',
	'price_AUD' => '290',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'bisulfite-conversion-package-for-RRBS',
	'meta_title' => 'Bisulfite conversion package for RRBS',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
	'modified' => '2019-12-16 10:57:53',
	'created' => '2016-11-18 23:22:25',
	'ProductsRelated' => array(
		'id' => '4332',
		'product_id' => '2648',
		'related_id' => '2853'
	),
	'Image' => array()
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '36',
	'name' => 'DNA Methylation Solutions',
	'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
	'slug' => 'dna-methylation-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 11:21:31',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '2827',
		'product_id' => '2648',
		'document_id' => '36'
	)
)
$sds = array(
	'id' => '2423',
	'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
	'language' => 'ja',
	'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
	'countries' => 'JP',
	'modified' => '2022-08-29 16:40:29',
	'created' => '2022-08-29 16:40:29',
	'ProductsSafetySheet' => array(
		'id' => '4119',
		'product_id' => '2648',
		'safety_sheet_id' => '2423'
	)
)
$publication = array(
	'id' => '3569',
	'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
	'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
	'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
	'date' => '2019-03-07',
	'pmid' => 'http://www.pubmed.gov/30846751',
	'doi' => '10.1038/s41598-019-40262-w',
	'modified' => '2019-05-09 17:33:09',
	'created' => '2019-03-21 14:12:08',
	'ProductsPublication' => array(
		'id' => '3335',
		'product_id' => '2648',
		'publication_id' => '3569'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/30846751" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
	'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2648',
			'antibody_id' => null,
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
			'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030034',
			'old_catalog_number' => '',
			'sf_code' => 'C02030034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '815',
			'price_USD' => '835',
			'price_GBP' => '745',
			'price_JPY' => '142400',
			'price_CNY' => '',
			'price_AUD' => '2090',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-kit',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-05-27 15:36:29',
			'created' => '2015-07-09 15:03:28',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			),
			(int) 8 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine'
$meta_description = 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.'
$meta_title = 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2648',
		'antibody_id' => null,
		'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
		'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '8 rxns',
		'catalog_number' => 'C02030034',
		'old_catalog_number' => '',
		'sf_code' => 'C02030034-',
		'type' => 'RFR',
		'search_order' => '04-undefined',
		'price_EUR' => '815',
		'price_USD' => '835',
		'price_GBP' => '745',
		'price_JPY' => '142400',
		'price_CNY' => '',
		'price_AUD' => '2090',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => true,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'premium-wgbs-kit',
		'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
		'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
		'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
		'modified' => '2020-05-27 15:36:29',
		'created' => '2015-07-09 15:03:28',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1892',
			'antibody_id' => null,
			'name' => 'Premium Bisulfite kit',
			'description' => '<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C02030030',
			'old_catalog_number' => '',
			'sf_code' => 'C02030030-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-bisulfite-kit-50-rxns',
			'meta_title' => 'Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Premium Bisulfite kit',
			'modified' => '2021-12-08 09:03:59',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2826',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 6',
			'description' => '<p style="text-align: justify;"><span>Theses 6 different indexes are designed for the <a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span><span></span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combines ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C05010032',
			'old_catalog_number' => '',
			'sf_code' => 'C05010032-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '740',
			'price_GBP' => '655',
			'price_JPY' => '129500',
			'price_CNY' => '',
			'price_AUD' => '1850',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-6',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 48 rxns | Diagenode',
			'meta_keywords' => '6 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 6 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:18',
			'created' => '2016-08-18 11:41:52',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 5 => array(
			'id' => '2827',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 12',
			'description' => '<p style="text-align: justify;"><span>Theses 12 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '96 rxns',
			'catalog_number' => 'C05010033',
			'old_catalog_number' => '',
			'sf_code' => 'C05010033-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '1260',
			'price_USD' => '1260',
			'price_GBP' => '1085',
			'price_JPY' => '220100',
			'price_CNY' => '',
			'price_AUD' => '3150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-12',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 96 rxns | Diagenode',
			'meta_keywords' => '12 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 12 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:02',
			'created' => '2016-08-18 11:49:42',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 6 => array(
			'id' => '2828',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 24',
			'description' => '<p style="text-align: justify;"><span>Theses 24 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '192 rxns',
			'catalog_number' => 'C05010034',
			'old_catalog_number' => '',
			'sf_code' => 'C05010034-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '2265',
			'price_USD' => '2265',
			'price_GBP' => '2050',
			'price_JPY' => '394300',
			'price_CNY' => '',
			'price_AUD' => '5665',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-24',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 192 rxns | Diagenode',
			'meta_keywords' => '24 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 24 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:34',
			'created' => '2016-08-18 11:49:49',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 7 => array(
			'id' => '2836',
			'antibody_id' => null,
			'name' => 'RRBS受託サービス (Reduced Representation Bisulfite Sequencing)',
			'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p>
<div class="extra-spaced">
<p>メチル化アレイとWGBS技術の長所を有するReduced representation bisulfite sequencing (RRBS) 技術は、あらゆる脊椎動物種で柔軟に使用できるため、大規模コホートの研究においても正確で費用対効果の高いアッセイを行えます。制限MspI酵素（CCGGターゲットサイト）を使用してゲノムを切断し、次にサイズを選択することで、濃縮されたDNAはプロモーターやCpGアイランドを含む関連するターゲットCpGリッチ領域を表します。またRRBS技術はゲノムの一部分を濃縮してメチル化解析を行うことによってシーケンス量を減らす手法でカバレッジが非常に高く、CpGメチル化解析に適しています。（ヒトサンプルで最大700万CpGが検出）。</p>
<h2>Genome-scale DNA methylation analysis</h2>
<ul class="square">
<li>Robust and cost-effective solution with&nbsp;reliable results</li>
<li>High coverage - up to 7 million CpGs detected in human samples</li>
<li>Single nucleotide resolution</li>
<li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li>
<li>Suitable for&nbsp;any vertebrate species</li>
</ul>
<h2>Complete end-to-end service</h2>
<ul class="square">
<li>From&nbsp;DNA QC to standard bioinformatics&nbsp;</li>
<li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2&nbsp;</a>widely adopted</li>
<li>As little as 25 ng&nbsp;for&nbsp;any vertebrate species</li>
<li>Differentially methylated site analysis&nbsp;</li>
</ul>
</div>
<div class="extra-spaced">
<h2></h2>
</div>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label1' => '詳細',
			'info1' => '<div class="row">
<div class="small-6 columns">
<h4>RRBS Service includes:</h4>
<table style="width: 905px;">
<tbody>
<tr>
<td style="width: 264px;"><strong>QC of the genomic DNA</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Measurement of DNA concentration&nbsp;</li>
<li>Assessment of DNA quality</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>MspI digestion</li>
<li>Library preparation (ends preparation, adaptor ligation)</li>
<li>Size selection</li>
<li>Sample pooling</li>
<li>Bisulfite conversion</li>
<li>Library amplification and clean-up</li>
<li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Deep sequencing</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li>
<li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li>
<li>7&nbsp;million CpGs (on average) for human samples</li>
<li>7-11x CpG coverage&nbsp;(on average) for human samples</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'バイオインフォマティック解析',
			'info2' => '<div class="row">
<div class="small-6 columns">
<table style="width: 895px;">
<thead>
<tr>
<th style="width: 262px;">
<h4><strong>Analysis</strong></h4>
</th>
<th style="width: 624px;">
<h4><strong>Features</strong></h4>
</th>
</tr>
</thead>
<tbody>
<tr>
<td style="width: 262px;"><strong>Standard</strong></td>
<td style="width: 624px;">
<ul>
<li>FASTQ raw data</li>
<li>FASTQC quality control insights</li>
<li>Alignment of bisulfite sequencing data against reference genome</li>
<li>Methylation calling and extraction</li>
<li>Summary statistics</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;"><strong>Differential&nbsp;methylation analysis<br /></strong></td>
<td style="width: 624px;">
<ul>
<li>Methylation level analysis</li>
<li>Differentially Methylated CpGs (DMCs) analysis</li>
<li>Differentially Methylated Regions (DMRs) analysis</li>
<li>Annotation of DMCs and DMRs for genomic regions (exons, introns, &hellip;)</li>
<li>Clustering analysis</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Gene ontology terms analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Enrichment analysis on gene associated with DMCs and DMRs</li>
<li>Get functional insights</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Pathway analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li>
<li>Get mechanistic insights</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '',
			'catalog_number' => 'G02020000',
			'old_catalog_number' => '',
			'sf_code' => '',
			'type' => 'ACC',
			'search_order' => '',
			'price_EUR' => '/',
			'price_USD' => '/',
			'price_GBP' => '/',
			'price_JPY' => '42800',
			'price_CNY' => '/',
			'price_AUD' => '/',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => true,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rrbs-service',
			'meta_title' => 'Reduced Representation Bisulfite Sequencing（RRBS）サービス| Diagenode',
			'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation',
			'meta_description' => 'Diagenode社のプレミアムRRBS（還元重亜硫酸塩シークエンシング）キットは、ほぼ90％のアライメント率、410万CpGのカバー率、重亜硫酸塩転換率はすべてのサンプルで約99.5％',
			'modified' => '2022-04-08 08:31:12',
			'created' => '2016-09-21 16:54:44',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 8 => array(
			'id' => '2853',
			'antibody_id' => null,
			'name' => 'Bisulfite conversion reagent for RRBS',
			'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030035',
			'old_catalog_number' => '',
			'sf_code' => 'C02030035-',
			'type' => 'REF',
			'search_order' => '01-undefined',
			'price_EUR' => '115',
			'price_USD' => '115',
			'price_GBP' => '110',
			'price_JPY' => '20900',
			'price_CNY' => '',
			'price_AUD' => '290',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bisulfite-conversion-package-for-RRBS',
			'meta_title' => 'Bisulfite conversion package for RRBS',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
			'modified' => '2019-12-16 10:57:53',
			'created' => '2016-11-18 23:22:25',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '6',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'メチル化DNA結合タンパク質',
			'description' => '<div class="row">
<div class="large-12 columns">MBD方法は、メチル化DNAに対するH6-GST-MBD融合タンパク質の非常に高い親和性に基づいています。 このタンパク質は、N末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）を含有します。 このH6-GST-MBD融合タンパク質を用いて、メチル化CpGを含むDNAを特異的に単離する事が可能です。<br /><br />DiagenodeのMethylCap&reg;キットは、二本鎖DNAの高濃縮と、メチル化CpG密度の関数における微分分画を可能にします。 分画はサンプルの複雑さを軽減し、次世代のシーケンシングを容易にします。 MethylCapアッセイに先立ち、DNAを最初に抽出し、Picoruptorソニケーターを用いて断片化します。<br />
<h3>概要</h3>
<p class="text-center"><br /><img src="https://www.diagenode.com/img/applications/methyl_binding_domain_overview.jpg" /></p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'slug' => 'methylbinding-domain-protein',
			'meta_keywords' => 'Epigenetic,Methylbinding Domain Protein,MBD,DNA methylation,DNA replication,MethylCap,MethylCap assay,',
			'meta_description' => 'Methylbinding Domain Protein(MBD) approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase',
			'meta_title' => 'Epigenetic Methylbinding Domain Protein(MBD) - DNA methylation | Diagenode',
			'modified' => '2019-03-22 12:32:12',
			'created' => '2015-06-02 17:05:42',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<p><br />Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Whole Genome Bisulfite Sequencing (WGBS) with our <a href="https://www.diagenode.com/en/p/premium-wgbs-kit">Premium WGBS Kit</a></li>
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2022-12-02 11:57:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '790',
			'name' => 'Premium WGBS kit',
			'description' => '<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<p><span>Whole Genome Bisulfite Sequencing </span><span>(WGBS) is a single nucleotide resolution technique that allows the user to study DNA methylation sites and their role in gene regulation. The Diagenode&rsquo;s </span><span>Premium WGBS Kit </span><span>is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for the preparation of bisulfite converted library from ChIP&rsquo;d samples in order to perform </span><span>ChIP-Bis-Sequencing</span><span>. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s Premium RRBS Kit (Cat. No.C02030032 and C02030033). </span></p>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Premium_WGBS_manual.pdf',
			'slug' => 'premium-wgbs-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:19:28',
			'created' => '2015-07-09 15:56:04',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '36',
			'name' => 'DNA Methylation Solutions',
			'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Brochure',
			'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
			'slug' => 'dna-methylation-brochure',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 11:21:31',
			'created' => '2015-07-03 16:05:27',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3742',
			'name' => 'Development and epigenetic plasticity of murine Müller glia.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of M&uuml;ller glia. To understand the role of mammalian M&uuml;ller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expression during differentiation of retinal progenitor cells (RPCs) into M&uuml;ller glia. We also analyzed the global epigenetic profile of adult M&uuml;ller glia. We observed significant changes in gene expression during differentiation of RPCs into M&uuml;ller glia in only a small group of genes. We found a high similarity between RPCs and M&uuml;ller glia on the transcriptomic and epigenomic levels. Our findings also indicate that M&uuml;ller glia are epigenetically very close to late-born retinal neurons, but not early-born retinal neurons. Importantly, we found that key genes required for phototransduction were highly methylated. Thus, our data suggest that M&uuml;ller glia are epigenetically very similar to late RPCs. Meanwhile, obstacles for regeneration of the entire mammalian retina from M&uuml;ller glia may consist of repressive chromatin and highly methylated DNA in the promoter regions of many genes required for the development of early-born retinal neurons. In addition, DNA demethylation may be required for proper reprogramming and differentiation of M&uuml;ller glia into rod photoreceptors.</p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>',
			'date' => '2019-07-02',
			'pmid' => 'http://www.pubmed.gov/31276697',
			'doi' => '10.1016/j.bbamcr.2019.06.019',
			'modified' => '2019-08-13 10:50:24',
			'created' => '2019-07-31 13:35:50',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3569',
			'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
			'date' => '2019-03-07',
			'pmid' => 'http://www.pubmed.gov/30846751',
			'doi' => '10.1038/s41598-019-40262-w',
			'modified' => '2019-05-09 17:33:09',
			'created' => '2019-03-21 14:12:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2422',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2424',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2418',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2417',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2421',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2420',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2419',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2423',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030030</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1892" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-premium-bisulfite-kit-40-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030031</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1893" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1893" id="CartAdd/1893Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1893" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-premium-bisulfite-kit-40-rxns" data-reveal-id="cartModal-1893" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-6"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2826" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2826" id="CartAdd/2826Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2826" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 6</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                     '740',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                    '740',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-6" data-reveal-id="cartModal-2826" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 6</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-12"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2827" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2827" id="CartAdd/2827Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2827" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 12</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                     '1260',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                    '1260',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-12" data-reveal-id="cartModal-2827" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 12</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-24"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2828" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2828" id="CartAdd/2828Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2828" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 24</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                     '2265',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                    '2265',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-24" data-reveal-id="cartModal-2828" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 24</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/rrbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">G02020000</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2836" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

    </div>
</div>

<form action="/en/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RWM1TjFTVlNVWUlrQjBQOHRxaFRWUT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
    <div class="row collapse">
        <h2>Service Information</h2>
    </div>

    <div class="small-12 large-12 columns">
        <h4>Which services are you interested in?</h4>
    </div>
    <div class="small-12 large-12 columns">
        <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/>

<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium EPIC Array" id="QuoteEpigenomicsServiceInfiniumEPICArray" /><label for="QuoteEpigenomicsServiceInfiniumEPICArray">Infinium EPIC Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div>
    </div>


    <div class="row collapse">
        <div class="small-12 medium-12 large-3 columns">
            <span class="prefix">Sample species</span>
        </div>
        <div class="small-12 medium-12 large-9 columns">
            <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/>        </div>
    </div>

    <div class="row collapse">
        <div class="small-12 medium-12 large-6 columns">
            <span class="prefix">Total number of samples (including replicates)</span>
        </div>
        <div class="small-12 medium-12 large-6 columns">
            <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/>        </div>
    </div>


<div class="row collapse">
    <h2>Contact Information</h2>
    <div class="small-3 large-2 columns">
        <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-2836').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-2836" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Project</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Describe your project" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha642430804f083"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-2836" class="alert button expand" form="Quote-2836" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-2836').attr('action','javascript:postToPardot(\'' + $('#Quote-2836').attr('action') + '\', 2836)');
    });

    $("#Quote-2836 #submit_btn-2836").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-2836 #submit_btn-2836").attr("disabled", true);
            $("#Quote-2836 #submit_btn-2836").html("Processing...");

            //submit the form
            $("#Quote-2836").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'US') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'CA') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'DE') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else {
        $("#Quote-2836 #state-2836").parent().parent().hide();
        $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
    }

    $("#Quote-2836 #country_selector_quote-2836.selectized").change(function() {
        if (this.value == 'US') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'CA') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'DE') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else {
            $("#Quote-2836 #state-2836").parent().parent().hide();
            $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
        }

    });
</script>
<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="rrbs-service" data-reveal-id="quoteModal-2836" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">RRBS Service (Reduced Representation Bisulfite ...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/bisulfite-conversion-package-for-RRBS"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030035</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2853" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2853" id="CartAdd/2853Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2853" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Bisulfite conversion reagent for RRBS</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                     '115',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                    '115',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="bisulfite-conversion-package-for-RRBS" data-reveal-id="cartModal-2853" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Bisulfite conversion reagent for RRBS</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '2853',
	'antibody_id' => null,
	'name' => 'Bisulfite conversion reagent for RRBS',
	'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '8 rxns',
	'catalog_number' => 'C02030035',
	'old_catalog_number' => '',
	'sf_code' => 'C02030035-',
	'type' => 'REF',
	'search_order' => '01-undefined',
	'price_EUR' => '115',
	'price_USD' => '115',
	'price_GBP' => '110',
	'price_JPY' => '20900',
	'price_CNY' => '',
	'price_AUD' => '290',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'bisulfite-conversion-package-for-RRBS',
	'meta_title' => 'Bisulfite conversion package for RRBS',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
	'modified' => '2019-12-16 10:57:53',
	'created' => '2016-11-18 23:22:25',
	'ProductsRelated' => array(
		'id' => '4332',
		'product_id' => '2648',
		'related_id' => '2853'
	),
	'Image' => array()
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '36',
	'name' => 'DNA Methylation Solutions',
	'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
	'slug' => 'dna-methylation-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 11:21:31',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '2827',
		'product_id' => '2648',
		'document_id' => '36'
	)
)
$sds = array(
	'id' => '2423',
	'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
	'language' => 'ja',
	'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
	'countries' => 'JP',
	'modified' => '2022-08-29 16:40:29',
	'created' => '2022-08-29 16:40:29',
	'ProductsSafetySheet' => array(
		'id' => '4119',
		'product_id' => '2648',
		'safety_sheet_id' => '2423'
	)
)
$publication = array(
	'id' => '3569',
	'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
	'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
	'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
	'date' => '2019-03-07',
	'pmid' => 'http://www.pubmed.gov/30846751',
	'doi' => '10.1038/s41598-019-40262-w',
	'modified' => '2019-05-09 17:33:09',
	'created' => '2019-03-21 14:12:08',
	'ProductsPublication' => array(
		'id' => '3335',
		'product_id' => '2648',
		'publication_id' => '3569'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/30846751" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
	'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2648',
			'antibody_id' => null,
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
			'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030034',
			'old_catalog_number' => '',
			'sf_code' => 'C02030034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '815',
			'price_USD' => '835',
			'price_GBP' => '745',
			'price_JPY' => '142400',
			'price_CNY' => '',
			'price_AUD' => '2090',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-kit',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-05-27 15:36:29',
			'created' => '2015-07-09 15:03:28',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			),
			(int) 8 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine'
$meta_description = 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.'
$meta_title = 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2648',
		'antibody_id' => null,
		'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
		'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '8 rxns',
		'catalog_number' => 'C02030034',
		'old_catalog_number' => '',
		'sf_code' => 'C02030034-',
		'type' => 'RFR',
		'search_order' => '04-undefined',
		'price_EUR' => '815',
		'price_USD' => '835',
		'price_GBP' => '745',
		'price_JPY' => '142400',
		'price_CNY' => '',
		'price_AUD' => '2090',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => true,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'premium-wgbs-kit',
		'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
		'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
		'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
		'modified' => '2020-05-27 15:36:29',
		'created' => '2015-07-09 15:03:28',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1892',
			'antibody_id' => null,
			'name' => 'Premium Bisulfite kit',
			'description' => '<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C02030030',
			'old_catalog_number' => '',
			'sf_code' => 'C02030030-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-bisulfite-kit-50-rxns',
			'meta_title' => 'Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Premium Bisulfite kit',
			'modified' => '2021-12-08 09:03:59',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '96 rxns',
			'catalog_number' => 'C02030033',
			'old_catalog_number' => '',
			'sf_code' => 'C02030033-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '4415',
			'price_USD' => '4385',
			'price_GBP' => '4085',
			'price_JPY' => '769000',
			'price_CNY' => '',
			'price_AUD' => '10965',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x96-96-rxns',
			'meta_title' => 'Premium RRBS Kit | Diagenode ',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries to efficiently analyze DNA methylation at the single nucleotide level. More cost effective than Whole Genome Bisulfite Sequencing (WGBS) and with greater coverage than microarray-based methods.',
			'modified' => '2022-12-05 11:22:21',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2826',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 6',
			'description' => '<p style="text-align: justify;"><span>Theses 6 different indexes are designed for the <a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span><span></span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combines ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '48 rxns',
			'catalog_number' => 'C05010032',
			'old_catalog_number' => '',
			'sf_code' => 'C05010032-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '740',
			'price_USD' => '740',
			'price_GBP' => '655',
			'price_JPY' => '129500',
			'price_CNY' => '',
			'price_AUD' => '1850',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-6',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 48 rxns | Diagenode',
			'meta_keywords' => '6 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 6 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:18',
			'created' => '2016-08-18 11:41:52',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 5 => array(
			'id' => '2827',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 12',
			'description' => '<p style="text-align: justify;"><span>Theses 12 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '96 rxns',
			'catalog_number' => 'C05010033',
			'old_catalog_number' => '',
			'sf_code' => 'C05010033-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '1260',
			'price_USD' => '1260',
			'price_GBP' => '1085',
			'price_JPY' => '220100',
			'price_CNY' => '',
			'price_AUD' => '3150',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-12',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 96 rxns | Diagenode',
			'meta_keywords' => '12 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 12 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:02',
			'created' => '2016-08-18 11:49:42',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 6 => array(
			'id' => '2828',
			'antibody_id' => null,
			'name' => 'Premium WGBS Indexes - 24',
			'description' => '<p style="text-align: justify;"><span>Theses 24 different indexes are designed for the </span><a href="../p/premium-wgbs-kit">Premium Whole Genome Bisulfite Sequencing (WGBS) Kit</a><span> and allow for preparing single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. They have&nbsp;also been validated for bisulfite- converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed indexes and master mix needed for genome-wide bisulfite sequencing to study&nbsp;DNA methylation sites and their role in gene regulation.</span></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong> - Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong> - Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong><br /> A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '192 rxns',
			'catalog_number' => 'C05010034',
			'old_catalog_number' => '',
			'sf_code' => 'C05010034-',
			'type' => 'FRE',
			'search_order' => '04-undefined',
			'price_EUR' => '2265',
			'price_USD' => '2265',
			'price_GBP' => '2050',
			'price_JPY' => '394300',
			'price_CNY' => '',
			'price_AUD' => '5665',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-Indexes-24',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS)  Indexes - 192 rxns | Diagenode',
			'meta_keywords' => '24 indexes multiplexing DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) indexes allow multiplexing of up to 24 samples. Genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-08-28 15:29:34',
			'created' => '2016-08-18 11:49:49',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 7 => array(
			'id' => '2836',
			'antibody_id' => null,
			'name' => 'RRBS受託サービス (Reduced Representation Bisulfite Sequencing)',
			'description' => '<p><a href="https://www.diagenode.com/en/quotes/quote?id=2836"><img alt="RRBS Service" src="https://www.diagenode.com/img/banners/b-page-promo-rrbs.png" /></a></p>
<div class="extra-spaced">
<p>メチル化アレイとWGBS技術の長所を有するReduced representation bisulfite sequencing (RRBS) 技術は、あらゆる脊椎動物種で柔軟に使用できるため、大規模コホートの研究においても正確で費用対効果の高いアッセイを行えます。制限MspI酵素（CCGGターゲットサイト）を使用してゲノムを切断し、次にサイズを選択することで、濃縮されたDNAはプロモーターやCpGアイランドを含む関連するターゲットCpGリッチ領域を表します。またRRBS技術はゲノムの一部分を濃縮してメチル化解析を行うことによってシーケンス量を減らす手法でカバレッジが非常に高く、CpGメチル化解析に適しています。（ヒトサンプルで最大700万CpGが検出）。</p>
<h2>Genome-scale DNA methylation analysis</h2>
<ul class="square">
<li>Robust and cost-effective solution with&nbsp;reliable results</li>
<li>High coverage - up to 7 million CpGs detected in human samples</li>
<li>Single nucleotide resolution</li>
<li>Detection of methylation patterns in CpG rich regions including promoters and CpG islands</li>
<li>Suitable for&nbsp;any vertebrate species</li>
</ul>
<h2>Complete end-to-end service</h2>
<ul class="square">
<li>From&nbsp;DNA QC to standard bioinformatics&nbsp;</li>
<li>Leveraging our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS kit V2&nbsp;</a>widely adopted</li>
<li>As little as 25 ng&nbsp;for&nbsp;any vertebrate species</li>
<li>Differentially methylated site analysis&nbsp;</li>
</ul>
</div>
<div class="extra-spaced">
<h2></h2>
</div>
<p><span><i class="fa fa-arrow-circle-right"></i> </span><a href="https://www.diagenode.com/en/categories/dna-methylation-profiling-services">See our other DNA Methylation Profiling Services</a></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label1' => '詳細',
			'info1' => '<div class="row">
<div class="small-6 columns">
<h4>RRBS Service includes:</h4>
<table style="width: 905px;">
<tbody>
<tr>
<td style="width: 264px;"><strong>QC of the genomic DNA</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Measurement of DNA concentration&nbsp;</li>
<li>Assessment of DNA quality</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Preparation of RRBS libraries</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>MspI digestion</li>
<li>Library preparation (ends preparation, adaptor ligation)</li>
<li>Size selection</li>
<li>Sample pooling</li>
<li>Bisulfite conversion</li>
<li>Library amplification and clean-up</li>
<li>QC of the RRBS library pool (DNA concentration, analysis of the pool profile)</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 264px;"><strong>Deep sequencing</strong></td>
<td style="width: 636px;">
<ul style="list-style-type: circle;">
<li>Samples are sequenced on an Illumina platform, paired-end reads, read length 50 bp (PE50)</li>
<li>40 million raw reads (on average) per sample when pooling 10 samples/lane</li>
<li>7&nbsp;million CpGs (on average) for human samples</li>
<li>7-11x CpG coverage&nbsp;(on average) for human samples</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'バイオインフォマティック解析',
			'info2' => '<div class="row">
<div class="small-6 columns">
<table style="width: 895px;">
<thead>
<tr>
<th style="width: 262px;">
<h4><strong>Analysis</strong></h4>
</th>
<th style="width: 624px;">
<h4><strong>Features</strong></h4>
</th>
</tr>
</thead>
<tbody>
<tr>
<td style="width: 262px;"><strong>Standard</strong></td>
<td style="width: 624px;">
<ul>
<li>FASTQ raw data</li>
<li>FASTQC quality control insights</li>
<li>Alignment of bisulfite sequencing data against reference genome</li>
<li>Methylation calling and extraction</li>
<li>Summary statistics</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;"><strong>Differential&nbsp;methylation analysis<br /></strong></td>
<td style="width: 624px;">
<ul>
<li>Methylation level analysis</li>
<li>Differentially Methylated CpGs (DMCs) analysis</li>
<li>Differentially Methylated Regions (DMRs) analysis</li>
<li>Annotation of DMCs and DMRs for genomic regions (exons, introns, &hellip;)</li>
<li>Clustering analysis</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Gene ontology terms analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Enrichment analysis on gene associated with DMCs and DMRs</li>
<li>Get functional insights</li>
</ul>
</td>
</tr>
<tr>
<td style="width: 262px;">
<p><strong>Pathway analysis</strong></p>
</td>
<td style="width: 624px;">
<ul>
<li>Identification of biological pathways in which genes associated with DMCs and DMRs may be over-represented (or under-represented)</li>
<li>Get mechanistic insights</li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label3' => '',
			'info3' => '<p></p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '',
			'catalog_number' => 'G02020000',
			'old_catalog_number' => '',
			'sf_code' => '',
			'type' => 'ACC',
			'search_order' => '',
			'price_EUR' => '/',
			'price_USD' => '/',
			'price_GBP' => '/',
			'price_JPY' => '42800',
			'price_CNY' => '/',
			'price_AUD' => '/',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => true,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => true,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'rrbs-service',
			'meta_title' => 'Reduced Representation Bisulfite Sequencing（RRBS）サービス| Diagenode',
			'meta_keywords' => 'RRBS,Reduced representation bisulfite sequencing,DNA methylation',
			'meta_description' => 'Diagenode社のプレミアムRRBS（還元重亜硫酸塩シークエンシング）キットは、ほぼ90％のアライメント率、410万CpGのカバー率、重亜硫酸塩転換率はすべてのサンプルで約99.5％',
			'modified' => '2022-04-08 08:31:12',
			'created' => '2016-09-21 16:54:44',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 8 => array(
			'id' => '2853',
			'antibody_id' => null,
			'name' => 'Bisulfite conversion reagent for RRBS',
			'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030035',
			'old_catalog_number' => '',
			'sf_code' => 'C02030035-',
			'type' => 'REF',
			'search_order' => '01-undefined',
			'price_EUR' => '115',
			'price_USD' => '115',
			'price_GBP' => '110',
			'price_JPY' => '20900',
			'price_CNY' => '',
			'price_AUD' => '290',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'bisulfite-conversion-package-for-RRBS',
			'meta_title' => 'Bisulfite conversion package for RRBS',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
			'modified' => '2019-12-16 10:57:53',
			'created' => '2016-11-18 23:22:25',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		)
	),
	'Application' => array(
		(int) 0 => array(
			'id' => '5',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'Bisulfite conversion',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">Bisulfite modification of DNA is the most commonly used, "<strong>gold standard</strong>" method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. T<span style="font-weight: 400;">his technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level.</span></div>
<div style="text-align: justify;" class="large-12 columns"></div>
<div style="text-align: justify;" class="large-12 columns">Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
<h3>How it works</h3>
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figure 1).
<p class="text-center"><img src="https://www.diagenode.com/img/applications/bisulfite.png" /><br />Figure 1: Overview of bisulfite conversion of DNA</p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'dna-bisulfite-conversion',
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies. Since bisulfite treatment introduces specific changes in the DNA sequence depending on the methylation status of individual cytosine residues',
			'meta_title' => 'Bisulfite sequencing(Bis-Seq) - Bisulfite conversion - DNA Methylation | Diagenode',
			'modified' => '2018-03-14 14:48:31',
			'created' => '2015-02-17 14:48:32',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1',
			'position' => '9',
			'parent_id' => null,
			'name' => 'DNA Methylation',
			'description' => '<div class="row">
<div class="large-12 columns">
<div style="text-align: justify;" class="small-12 medium-8 large-8 columns">
<h2>Complete solutions for DNA methylation studies</h2>
<p>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements.</p>
</div>
<div class="small-12 medium-4 large-4 columns text-center"><a href="../landing-pages/dna-methylation-grant-applications"><img src="https://www.diagenode.com/img/banners/banner-dna-grant.png" alt="" /></a></div>
<div style="text-align: justify;" class="small-12 medium-12 large-12 columns">
<p>DNA methylation was the first discovered epigenetic mark and is the most widely studied topic in epigenetics. <em>In vivo</em>, DNA is methylated following DNA replication and is involved in a number of biological processes including the regulation of imprinted genes, X chromosome inactivation. and tumor suppressor gene silencing in cancer cells. Methylation often occurs in cytosine-guanine rich regions of DNA (CpG islands), which are commonly upstream of promoter regions.</p>
</div>
<div class="small-12 medium-12 large-12 columns"><br /><br />
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#dnamethyl"><i class="fa fa-caret-right"></i> Learn more</a>
<div id="dnamethyl" class="content">5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.
<p class="text-center"><img src="https://www.diagenode.com/img/categories/kits_dna/dna_methylation_variants.jpg" /></p>
</div>
</li>
</ul>
<br />
<h2>Main DNA methylation technologies</h2>
<p style="text-align: justify;">Overview of the <span style="font-weight: 400;">three main approaches for studying DNA methylation.</span></p>
<div class="row">
<ol>
<li style="font-weight: 400;"><span style="font-weight: 400;">Chemical modification with bisulfite &ndash; Bisulfite conversion</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Enrichment of methylated DNA (including MeDIP and MBD)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Treatment with methylation-sensitive or dependent restriction enzymes</span></li>
</ol>
<p><span style="font-weight: 400;">&nbsp;</span></p>
<div class="row">
<table>
<thead>
<tr>
<th></th>
<th>Description</th>
<th width="350">Features</th>
</tr>
</thead>
<tbody>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td><span style="font-weight: 400;">Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Single nucleotide resolution</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Quantitative analysis - methylation rate (%)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Gold standard and well studied</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><b>Methylated DNA enrichment</b></td>
<td><span style="font-weight: 400;">(Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Resolution depends on the fragment size of the enriched methylated DNA (300 bp)</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Qualitative analysis</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Compatible with automation</span></li>
</ul>
</td>
</tr>
<tr>
<td><strong>Restriction enzyme-based digestion</strong></td>
<td><span style="font-weight: 400;">Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.</span></td>
<td>
<ul style="list-style-type: circle;">
<li style="font-weight: 400;"><span style="font-weight: 400;">Determination of methylation status is limited by the enzyme recognition site</span></li>
<li style="font-weight: 400;"><span style="font-weight: 400;">Easy to use</span></li>
</ul>
</td>
</tr>
</tbody>
</table>
</div>
</div>
<div class="row"></div>
</div>
</div>
<div class="large-12 columns"></div>
</div>',
			'in_footer' => true,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'slug' => 'epigenetics-dna-methylation',
			'meta_keywords' => 'Epigenetics, DNA Methylation,5-hmC monoclonal antibody,hMeDIP,Bisulfite conversion,Methylated DNA immunoprecipitation',
			'meta_description' => 'Complete, optimized solutions for analyzing DNA methylation manually or on our automated system.',
			'meta_title' => 'DNA Methylation - Bisulfite sequencing - Epigenetics | Diagenode',
			'modified' => '2019-03-25 10:07:27',
			'created' => '2015-05-03 13:47:53',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '6',
			'position' => '10',
			'parent_id' => '1',
			'name' => 'メチル化DNA結合タンパク質',
			'description' => '<div class="row">
<div class="large-12 columns">MBD方法は、メチル化DNAに対するH6-GST-MBD融合タンパク質の非常に高い親和性に基づいています。 このタンパク質は、N末端His6タグを含むグルタチオン-S-トランスフェラーゼ（GST）とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン（MBD）を含有します。 このH6-GST-MBD融合タンパク質を用いて、メチル化CpGを含むDNAを特異的に単離する事が可能です。<br /><br />DiagenodeのMethylCap&reg;キットは、二本鎖DNAの高濃縮と、メチル化CpG密度の関数における微分分画を可能にします。 分画はサンプルの複雑さを軽減し、次世代のシーケンシングを容易にします。 MethylCapアッセイに先立ち、DNAを最初に抽出し、Picoruptorソニケーターを用いて断片化します。<br />
<h3>概要</h3>
<p class="text-center"><br /><img src="https://www.diagenode.com/img/applications/methyl_binding_domain_overview.jpg" /></p>
</div>
</div>',
			'in_footer' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'slug' => 'methylbinding-domain-protein',
			'meta_keywords' => 'Epigenetic,Methylbinding Domain Protein,MBD,DNA methylation,DNA replication,MethylCap,MethylCap assay,',
			'meta_description' => 'Methylbinding Domain Protein(MBD) approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase',
			'meta_title' => 'Epigenetic Methylbinding Domain Protein(MBD) - DNA methylation | Diagenode',
			'modified' => '2019-03-22 12:32:12',
			'created' => '2015-06-02 17:05:42',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '54',
			'position' => '1',
			'parent_id' => '12',
			'name' => 'Bisulfite conversion',
			'description' => '<p><br />Sodium bisulfite conversion of genomic DNA is the most commonly used&nbsp;method for DNA methylation studies providing <strong>single nucleotide resolution</strong>. It enables <span>to differentiate and detect unmethylated versus methylated cytosines.&nbsp;This procedure can then be followed either by <strong>PCR amplification</strong> or&nbsp;<strong>next generation sequencing</strong> to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.</span></p>
<h2>How it works</h2>
<p style="text-align: left;">Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged.&nbsp;<span>The DNA is then amplified by PCR where the uracils are converted to thymines.&nbsp;</span></p>
<p style="text-align: center;"><span></span></p>
<p><img src="https://www.diagenode.com/img/categories/bisulfite-conversion/bisulfite-conversion-acgautac.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<h2>Advantages</h2>
<ul class="nobullet" style="font-size: 19px;">
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;</strong><strong>Single nucleotide</strong> resolution</li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;Gene-specific&nbsp;</strong>and&nbsp;<strong>genome-wide</strong><span>&nbsp;analyses</span></li>
<li><i class="fa fa-arrow-circle-right"></i><strong>&nbsp;NGS</strong><span>&nbsp;</span>compatible</li>
</ul>
<h2>Downstream analysis techniques</h2>
<ul class="square">
<li>Whole Genome Bisulfite Sequencing (WGBS) with our <a href="https://www.diagenode.com/en/p/premium-wgbs-kit">Premium WGBS Kit</a></li>
<li>Reduced Representation Bisulfite Sequencing (RRBS) with our <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS Kit&nbsp;V2</a></li>
<li>Bisulfite conversion with our <a href="https://www.diagenode.com/en/p/premium-bisulfite-kit-50-rxns">Premium Bisulfite Kit</a> followed by qPCR, Sanger, Pyrosequencing</li>
</ul>
<p></p>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => true,
			'hide' => false,
			'all_format' => false,
			'is_antibody' => false,
			'slug' => 'bisulfite-conversion',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Bisulfite conversion,bisulfite sequencing,DNA methylation,Epigenetics ,next-generation sequencing',
			'meta_description' => 'Bisulfitre conversion is the gold standard method for DNA methylation studies at a single base pair resolution. Prepare your libraries for bisulfite sequencing with one of our Premium kits.',
			'meta_title' => 'DNA Methylation - Bisulfite conversion - Epigenetics | Diagenode ',
			'modified' => '2022-12-02 11:57:50',
			'created' => '2015-07-08 09:42:42',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '790',
			'name' => 'Premium WGBS kit',
			'description' => '<div class="page" title="Page 4">
<div class="layoutArea">
<div class="column">
<p><span>Whole Genome Bisulfite Sequencing </span><span>(WGBS) is a single nucleotide resolution technique that allows the user to study DNA methylation sites and their role in gene regulation. The Diagenode&rsquo;s </span><span>Premium WGBS Kit </span><span>is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for the preparation of bisulfite converted library from ChIP&rsquo;d samples in order to perform </span><span>ChIP-Bis-Sequencing</span><span>. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s Premium RRBS Kit (Cat. No.C02030032 and C02030033). </span></p>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Manual',
			'url' => 'files/products/kits/Premium_WGBS_manual.pdf',
			'slug' => 'premium-wgbs-manual',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 12:19:28',
			'created' => '2015-07-09 15:56:04',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '36',
			'name' => 'DNA Methylation Solutions',
			'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
			'image_id' => null,
			'type' => 'Brochure',
			'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
			'slug' => 'dna-methylation-brochure',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2015-09-01 11:21:31',
			'created' => '2015-07-03 16:05:27',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '1778',
			'name' => 'product/kits/methyl-kit-icon.png',
			'alt' => 'Methylation kit icon',
			'modified' => '2019-04-23 15:17:01',
			'created' => '2018-03-15 15:52:12',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(
		(int) 0 => array(
			'id' => '3742',
			'name' => 'Development and epigenetic plasticity of murine Müller glia.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The ability to regenerate the entire retina and restore lost sight after injury is found in some species and relies mostly on the epigenetic plasticity of M&uuml;ller glia. To understand the role of mammalian M&uuml;ller glia as a source of progenitors for retinal regeneration, we investigated changes in gene expression during differentiation of retinal progenitor cells (RPCs) into M&uuml;ller glia. We also analyzed the global epigenetic profile of adult M&uuml;ller glia. We observed significant changes in gene expression during differentiation of RPCs into M&uuml;ller glia in only a small group of genes. We found a high similarity between RPCs and M&uuml;ller glia on the transcriptomic and epigenomic levels. Our findings also indicate that M&uuml;ller glia are epigenetically very close to late-born retinal neurons, but not early-born retinal neurons. Importantly, we found that key genes required for phototransduction were highly methylated. Thus, our data suggest that M&uuml;ller glia are epigenetically very similar to late RPCs. Meanwhile, obstacles for regeneration of the entire mammalian retina from M&uuml;ller glia may consist of repressive chromatin and highly methylated DNA in the promoter regions of many genes required for the development of early-born retinal neurons. In addition, DNA demethylation may be required for proper reprogramming and differentiation of M&uuml;ller glia into rod photoreceptors.</p>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>
<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>',
			'date' => '2019-07-02',
			'pmid' => 'http://www.pubmed.gov/31276697',
			'doi' => '10.1016/j.bbamcr.2019.06.019',
			'modified' => '2019-08-13 10:50:24',
			'created' => '2019-07-31 13:35:50',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '3569',
			'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
			'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
			'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
			'date' => '2019-03-07',
			'pmid' => 'http://www.pubmed.gov/30846751',
			'doi' => '10.1038/s41598-019-40262-w',
			'modified' => '2019-05-09 17:33:09',
			'created' => '2019-03-21 14:12:08',
			'ProductsPublication' => array(
				[maximum depth reached]
			)
		)
	),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '2422',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-GB-en-1_0.pdf',
			'countries' => 'GB',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '2424',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-US-en-1_0.pdf',
			'countries' => 'US',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '2418',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-nl-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '2417',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-BE-fr-1_0.pdf',
			'countries' => 'BE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '2421',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-FR-fr-1_0.pdf',
			'countries' => 'FR',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '2420',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-ES-es-1_0.pdf',
			'countries' => 'ES',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '2419',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-DE-de-1_0.pdf',
			'countries' => 'DE',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '2423',
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
			'countries' => 'JP',
			'modified' => '2022-08-29 16:40:29',
			'created' => '2022-08-29 16:40:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-bisulfite-kit-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030030</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1892" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1892" id="CartAdd/1892Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1892" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium Bisulfite kit',
                    'C02030030',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-bisulfite-kit-50-rxns" data-reveal-id="cartModal-1892" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/auto-premium-bisulfite-kit-40-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030031</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1893" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1893" id="CartAdd/1893Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1893" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto Premium Bisulfite kit</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                     '225',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Auto Premium Bisulfite kit',
                    'C02030031',
                    '225',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-premium-bisulfite-kit-40-rxns" data-reveal-id="cartModal-1893" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Auto Premium Bisulfite kit</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x24-24-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (24 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-rrbs-kit-x96-96-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium RRBS kit (96 rxns) - replaced by Premiu...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-6"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010032</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2826" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2826" id="CartAdd/2826Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2826" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 6</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                     '740',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 6',
                    'C05010032',
                    '740',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-6" data-reveal-id="cartModal-2826" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 6</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-12"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010033</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2827" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2827" id="CartAdd/2827Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2827" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 12</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                     '1260',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 12',
                    'C05010033',
                    '1260',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-12" data-reveal-id="cartModal-2827" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 12</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/premium-wgbs-Indexes-24"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C05010034</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2828" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2828" id="CartAdd/2828Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2828" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Premium WGBS Indexes - 24</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                     '2265',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Premium WGBS Indexes - 24',
                    'C05010034',
                    '2265',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="premium-wgbs-Indexes-24" data-reveal-id="cartModal-2828" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Premium WGBS Indexes - 24</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/rrbs-service"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">G02020000</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-2836" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>our epigenomics services</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>

    </div>
</div>

<form action="/en/quotes/quote?id=2836" id="Quote-2836" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="2836" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="RWM1TjFTVlNVWUlrQjBQOHRxaFRWUT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="rrbs-service" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
    <div class="row collapse">
        <h2>Service Information</h2>
    </div>

    <div class="small-12 large-12 columns">
        <h4>Which services are you interested in?</h4>
    </div>
    <div class="small-12 large-12 columns">
        <input type="hidden" name="data[Quote][epigenomics_service]" value="" id="QuoteEpigenomicsService"/>

<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ChIP-seq" id="QuoteEpigenomicsServiceChIPSeq" /><label for="QuoteEpigenomicsServiceChIPSeq">ChIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="ATAC-seq" id="QuoteEpigenomicsServiceATACSeq" /><label for="QuoteEpigenomicsServiceATACSeq">ATAC-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RRBS" id="QuoteEpigenomicsServiceRRBS" /><label for="QuoteEpigenomicsServiceRRBS">RRBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="WGBS" id="QuoteEpigenomicsServiceWGBS" /><label for="QuoteEpigenomicsServiceWGBS">WGBS</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="MeDIP-seq" id="QuoteEpigenomicsServiceMeDIPSeq" /><label for="QuoteEpigenomicsServiceMeDIPSeq">MeDIP-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Targeted DNA methylation analysis" id="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis" /><label for="QuoteEpigenomicsServiceTargetedDNAMethylationAnalysis">Targeted DNA methylation analysis</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium EPIC Array" id="QuoteEpigenomicsServiceInfiniumEPICArray" /><label for="QuoteEpigenomicsServiceInfiniumEPICArray">Infinium EPIC Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Infinium Mouse Methylation Array" id="QuoteEpigenomicsServiceInfiniumMouseMethylationArray" /><label for="QuoteEpigenomicsServiceInfiniumMouseMethylationArray">Infinium Mouse Methylation Array</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="RNA-seq" id="QuoteEpigenomicsServiceRNASeq" /><label for="QuoteEpigenomicsServiceRNASeq">RNA-seq</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Bioinformatics" id="QuoteEpigenomicsServiceBioinformatics" /><label for="QuoteEpigenomicsServiceBioinformatics">Bioinformatics</label></div>
<div class="checkbox"><input type="checkbox" name="data[Quote][epigenomics_service][]" value="Data mining" id="QuoteEpigenomicsServiceDataMining" /><label for="QuoteEpigenomicsServiceDataMining">Data mining</label></div>
    </div>


    <div class="row collapse">
        <div class="small-12 medium-12 large-3 columns">
            <span class="prefix">Sample species</span>
        </div>
        <div class="small-12 medium-12 large-9 columns">
            <input name="data[Quote][sample_species]" maxlength="510" type="text" id="QuoteSampleSpecies"/>        </div>
    </div>

    <div class="row collapse">
        <div class="small-12 medium-12 large-6 columns">
            <span class="prefix">Total number of samples (including replicates)</span>
        </div>
        <div class="small-12 medium-12 large-6 columns">
            <input name="data[Quote][number_samples]" maxlength="255" type="text" id="QuoteNumberSamples"/>        </div>
    </div>


<div class="row collapse">
    <h2>Contact Information</h2>
    <div class="small-3 large-2 columns">
        <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Phone number</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">City</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/>    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-2836">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d&#039;Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People&#039;s Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People&#039;s Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
    $('#country_selector_quote-2836').selectize();
</script><br />
    </div>
</div>
<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">State</span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][state]" id="state-2836" maxlength="3" type="text"/><br />
    </div>
</div>

<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/>    </div>
</div>
<div class="row collapse" id="email_v">
    <div class="small-3 large-2 columns">
        <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
    </div>
    <div class="small-9 large-10 columns">
        <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/>    </div>
</div>


<div class="row collapse">
    <div class="small-3 large-2 columns">
        <span class="prefix">Project</span>
    </div>
    <div class="small-9 large-10 columns">
        <textarea name="data[Quote][comment]" placeholder="Describe your project" cols="30" rows="6" id="QuoteComment"></textarea>    </div>
</div>

<!------------SERVICES PARTICULAR FORM START---------------->


<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->


<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <div class="recaptcha"><div id="recaptcha642430804f083"></div></div>    </div>
</div>
<br />

<div class="row collapse">
    <div class="small-3 large-2 columns">
    </div>
    <div class="small-9 large-10 columns">
        <button id="submit_btn-2836" class="alert button expand" form="Quote-2836" type="submit">Contact me</button>    </div>
</div>
</form><script>
    var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';

    function postToPardot(formAction, id) {
        $('#pardot-form-handler').load(function(){
            $('#Quote-' + id).attr('action', formAction);
            $('#Quote-' + id).submit();
        });
        $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
        
    }
    $(document).ready(function() {
        $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
		$('#Quote-2836').attr('action','javascript:postToPardot(\'' + $('#Quote-2836').attr('action') + '\', 2836)');
    });

    $("#Quote-2836 #submit_btn-2836").click(function (e) {
            
        if($(this).closest('form')[0].checkValidity()){
            e.preventDefault();
            
            //disable the submit button
            $("#Quote-2836 #submit_btn-2836").attr("disabled", true);
            $("#Quote-2836 #submit_btn-2836").html("Processing...");

            //submit the form
            $("#Quote-2836").submit();
        } 
    })
</script>
<script>
    if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'US') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'CA') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else if ($("#Quote-2836 #country_selector_quote-2836.selectized").val() == 'DE') {
        var val = $("#state-2836").val();
        $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
        if (val.length == 2) {
            $("#state-2836").val(val);
        }
        $("#state-2836").parent().parent().show();
    } else {
        $("#Quote-2836 #state-2836").parent().parent().hide();
        $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
    }

    $("#Quote-2836 #country_selector_quote-2836.selectized").change(function() {
        if (this.value == 'US') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'CA') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else if (this.value == 'DE') {
            $("#Quote-2836 #state-2836").replaceWith('<select name="data[Quote][state]" id="state-2836" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
            $("#Quote-2836 #state-2836").parent().parent().show();
        } else {
            $("#Quote-2836 #state-2836").parent().parent().hide();
            $("#Quote-2836 #state-2836").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-2836" value="">');
        }

    });
</script>
<a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: QUOTE MODAL --><a href="#" id="rrbs-service" data-reveal-id="quoteModal-2836" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">RRBS Service (Reduced Representation Bisulfite ...</h6>
        </div>

    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/en/p/bisulfite-conversion-package-for-RRBS"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C02030035</span>
            </div>
            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2853" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2853" id="CartAdd/2853Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2853" id="CartProductId"/>
<div class="row">
    <div class="small-12 medium-12 large-12 columns">
        
        <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Bisulfite conversion reagent for RRBS</strong>  to my shopping cart.</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                     '115',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('Bisulfite conversion reagent for RRBS',
                    'C02030035',
                    '115',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="bisulfite-conversion-package-for-RRBS" data-reveal-id="cartModal-2853" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">Bisulfite conversion reagent for RRBS</h6>
        </div>

    </div>
</li>
'
$related = array(
	'id' => '2853',
	'antibody_id' => null,
	'name' => 'Bisulfite conversion reagent for RRBS',
	'description' => '<p style="text-align: justify;"><span>This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions.</span></p>
<p style="text-align: justify;"><span>Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the&nbsp;</span><strong>single nucleotide level</strong><span>&nbsp;without the higher costs associated with whole genome bisulfite sequencing. By cutting the genome using the restriction MspI enzyme (CCGG target sites) followed by size selection, DNA is enriched to represent CpG-rich regions (including CpG islands), in which DNA methylation marks are typically found. Thus, RRBS provides a&nbsp;</span><strong>cost-effective</strong><span>&nbsp;method for analyzing DNA methylation by reducing the part of the genome that actually needs to be sequenced and focusing on relevant CpG islands.</span></p>',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '8 rxns',
	'catalog_number' => 'C02030035',
	'old_catalog_number' => '',
	'sf_code' => 'C02030035-',
	'type' => 'REF',
	'search_order' => '01-undefined',
	'price_EUR' => '115',
	'price_USD' => '115',
	'price_GBP' => '110',
	'price_JPY' => '20900',
	'price_CNY' => '',
	'price_AUD' => '290',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'bisulfite-conversion-package-for-RRBS',
	'meta_title' => 'Bisulfite conversion package for RRBS',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'Bisulfite conversion package for RRBS is compatible with the Premium RRBS kit and allows for performing additional bisulfite conversion reactions.',
	'modified' => '2019-12-16 10:57:53',
	'created' => '2016-11-18 23:22:25',
	'ProductsRelated' => array(
		'id' => '4332',
		'product_id' => '2648',
		'related_id' => '2853'
	),
	'Image' => array()
)
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
	'id' => '36',
	'name' => 'DNA Methylation Solutions',
	'description' => '<div class="page" title="Page 2">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>Complete solution for DNA methylation studies! </span></p>
<p><span>Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data between samples and experiments. Diagenode offers sonication instruments, reagent kits, high quality antibodies, and high-throughput automation capability to address all of your specific DNA methylation analysis requirements. </span></p>
</div>
</div>
</div>
</div>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/DNA_Methylation_Brochure.pdf',
	'slug' => 'dna-methylation-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2015-09-01 11:21:31',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '2827',
		'product_id' => '2648',
		'document_id' => '36'
	)
)
$sds = array(
	'id' => '2423',
	'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja',
	'language' => 'ja',
	'url' => 'files/SDS/WGBS/SDS-C02030034-Premium_Whole_Genome_Bisulfite_Sequencing_WGBS_kit-JP-ja-1_0.pdf',
	'countries' => 'JP',
	'modified' => '2022-08-29 16:40:29',
	'created' => '2022-08-29 16:40:29',
	'ProductsSafetySheet' => array(
		'id' => '4119',
		'product_id' => '2648',
		'safety_sheet_id' => '2423'
	)
)
$publication = array(
	'id' => '3569',
	'name' => 'The epigenetic basis for the impaired ability of adult murine retinal pigment epithelium cells to regenerate retinal tissue.',
	'authors' => 'Dvoriantchikova G, Seemungal RJ, Ivanov D',
	'description' => '<p>The epigenetic plasticity of amphibian retinal pigment epithelium (RPE) allows them to regenerate the entire retina, a trait known to be absent in mammals. In this study, we investigated the epigenetic plasticity of adult murine RPE to identify possible mechanisms that prevent mammalian RPE from regenerating retinal tissue. RPE were analyzed using microarray, ChIP-seq, and whole-genome bisulfite sequencing approaches. We found that the majority of key genes required for progenitor phenotypes were in a permissive chromatin state and unmethylated in RPE. We observed that the majority of non-photoreceptor genes had promoters in a repressive chromatin state, but these promoters were in unmethylated or low-methylated regions. Meanwhile, the majority of promoters for photoreceptor genes were found in a permissive chromatin state, but were highly-methylated. Methylome states of photoreceptor-related genes in adult RPE and embryonic retina (which mostly contain progenitors) were very similar. However, promoters of these genes were demethylated and activated during retinal development. Our data suggest that, epigenetically, adult murine RPE cells are a progenitor-like cell type. Most likely two mechanisms prevent adult RPE from reprogramming and differentiating into retinal neurons: 1) repressive chromatin in the promoter regions of non-photoreceptor retinal neuron genes; 2) highly-methylated promoters of photoreceptor-related genes.</p>',
	'date' => '2019-03-07',
	'pmid' => 'http://www.pubmed.gov/30846751',
	'doi' => '10.1038/s41598-019-40262-w',
	'modified' => '2019-05-09 17:33:09',
	'created' => '2019-03-21 14:12:08',
	'ProductsPublication' => array(
		'id' => '3335',
		'product_id' => '2648',
		'publication_id' => '3569'
	)
)
$externalLink = ' <a href="http://www.pubmed.gov/30846751" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
	'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
	'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
	'product' => array(
		'Product' => array(
			'id' => '2648',
			'antibody_id' => null,
			'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
			'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '8 rxns',
			'catalog_number' => 'C02030034',
			'old_catalog_number' => '',
			'sf_code' => 'C02030034-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '815',
			'price_USD' => '835',
			'price_GBP' => '745',
			'price_JPY' => '142400',
			'price_CNY' => '',
			'price_AUD' => '2090',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-wgbs-kit',
			'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
			'modified' => '2020-05-27 15:36:29',
			'created' => '2015-07-09 15:03:28',
			'locale' => 'eng'
		),
		'Antibody' => array(
			'host' => '*****',
			'id' => null,
			'name' => null,
			'description' => null,
			'clonality' => null,
			'isotype' => null,
			'lot' => null,
			'concentration' => null,
			'reactivity' => null,
			'type' => null,
			'purity' => null,
			'classification' => null,
			'application_table' => null,
			'storage_conditions' => null,
			'storage_buffer' => null,
			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
			'modified' => null,
			'created' => null,
			'select_label' => null
		),
		'Slave' => array(),
		'Group' => array(),
		'Related' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			),
			(int) 8 => array(
				[maximum depth reached]
			)
		),
		'Application' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			)
		),
		'Category' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Document' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Feature' => array(),
		'Image' => array(
			(int) 0 => array(
				[maximum depth reached]
			)
		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			)
		),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
			),
			(int) 2 => array(
				[maximum depth reached]
			),
			(int) 3 => array(
				[maximum depth reached]
			),
			(int) 4 => array(
				[maximum depth reached]
			),
			(int) 5 => array(
				[maximum depth reached]
			),
			(int) 6 => array(
				[maximum depth reached]
			),
			(int) 7 => array(
				[maximum depth reached]
			)
		)
	)
)
$language = 'en'
$meta_keywords = 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine'
$meta_description = 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.'
$meta_title = 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode'
$product = array(
	'Product' => array(
		'id' => '2648',
		'antibody_id' => null,
		'name' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit',
		'description' => '<ul>
<li><strong>Genome-wide screening for DNA methylation</strong><span>&nbsp;</span>- Library Preparation of bisulfite-converted DNA from whole genomes</li>
<li><strong>Compatible with ChIP-Bis-Seq</strong><span>&nbsp;</span>- Combining ChIP and DNA methylation profiles in one assay</li>
<li><strong>Complete kit</strong><span>&nbsp;</span>&ndash; Bisulfite conversion reagents and library preparation reagents</li>
</ul>
<p style="text-align: justify;"><span>The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina&reg; platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode&rsquo;s&nbsp;</span><a href="../p/premium-rrbs-kit-x24-24-rxns">Premium RRBS Kit</a><span>.</span></p>
<p style="text-align: justify;"><span>Looking for multiplexing? <a href="../p/premium-wgbs-Indexes-12">Check out our Premium WGBS indexes</a></span></p>
<h3 style="text-align: justify;"><span>Characteristics</span></h3>
<p><img src="https://www.diagenode.com/img/product/kits/WGBS_RRBS_comparison.png" alt="Diagenode_WGBS_RRBS_Comparison" width="1903" height="776" /></p>
<p><em><strong>Figure 1: Coverage of Diagenode&rsquo;s Premium WGBS Kit (A) and Premium RRBS Kit (B).</strong></em><br /><em>WGBS was performed using the Premium Whole Genome Bisulfite Sequencing (WGBS) kit.&nbsp;RRBS was performed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) kit. For both, after sequencing, reads were aligned on the mm10 reference genome. Visualization of the alignment bam files on Integrative Genome Viewer (IGV) shows excellent coverage&nbsp;(A) of the whole genome using WGBS and&nbsp;(B) of the CpGs areas using RRBS.</em></p>
<p></p>
<p><img src="https://www.diagenode.com/img/product/kits/premium-wgbs-kit-nup210_cpg.jpg" alt="ChIP-Bis-sequencing results" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><em><strong>Figure&nbsp;2: ChIP-Bis-sequencing results obtained with the Diagenode&rsquo;s Premium WGBS Kit</strong></em><br /><em>A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode&rsquo;s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP&rsquo;d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.</em></p>',
		'label1' => '',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '8 rxns',
		'catalog_number' => 'C02030034',
		'old_catalog_number' => '',
		'sf_code' => 'C02030034-',
		'type' => 'RFR',
		'search_order' => '04-undefined',
		'price_EUR' => '815',
		'price_USD' => '835',
		'price_GBP' => '745',
		'price_JPY' => '142400',
		'price_CNY' => '',
		'price_AUD' => '2090',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => true,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'premium-wgbs-kit',
		'meta_title' => 'Premium Whole Genome Bisulfite Sequencing (WGBS) kit | Diagenode',
		'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq ChIP-Bis-Seq WGBS whole genome bisulfite sequencing bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
		'meta_description' => 'The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit analyzes genome-wide DNA methylation at single nulceotide level.',
		'modified' => '2020-05-27 15:36:29',
		'created' => '2015-07-09 15:03:28',
		'locale' => 'eng'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => null,
		'name' => null,
		'description' => null,
		'clonality' => null,
		'isotype' => null,
		'lot' => null,
		'concentration' => null,
		'reactivity' => null,
		'type' => null,
		'purity' => null,
		'classification' => null,
		'application_table' => null,
		'storage_conditions' => null,
		'storage_buffer' => null,
		'precautions' => null,
		'uniprot_acc' => null,
		'slug' => null,
		'meta_keywords' => null,
		'meta_description' => null,
		'modified' => null,
		'created' => null,
		'select_label' => null
	),
	'Slave' => array(),
	'Group' => array(),
	'Related' => array(
		(int) 0 => array(
			'id' => '1892',
			'antibody_id' => null,
			'name' => 'Premium Bisulfite kit',
			'description' => '<p style="text-align: center;"><strong>Make your Bisulfite conversion now in only 60 minutes !</strong></p>
<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => '',
			'info1' => '',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '50 rxns',
			'catalog_number' => 'C02030030',
			'old_catalog_number' => '',
			'sf_code' => 'C02030030-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => false,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-bisulfite-kit-50-rxns',
			'meta_title' => 'Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Premium Bisulfite kit',
			'modified' => '2021-12-08 09:03:59',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '1893',
			'antibody_id' => null,
			'name' => 'Auto Premium Bisulfite kit',
			'description' => '<p>Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p><strong>Bisulfite Conversion efficiency in TERTBS1 and CTS56 genomic regions.</strong></p>
<p>The figure shows the conversion efficiency in TERTBS1 and CTS56 genomic regions when using 1 &mu;g, 500ng and 100 ng of genomic DNA. Between 1 and 5 clones were analyzed for the different amounts of genomic DNA</p>
<p><img src="https://www.diagenode.com/img/product/kits/auto-premium-bisulfite-clone.png" alt="Clone" /></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '40 rxns',
			'catalog_number' => 'C02030031',
			'old_catalog_number' => '',
			'sf_code' => 'C02030031-',
			'type' => 'REF',
			'search_order' => '04-undefined',
			'price_EUR' => '240',
			'price_USD' => '225',
			'price_GBP' => '220',
			'price_JPY' => '41900',
			'price_CNY' => '',
			'price_AUD' => '565',
			'country' => 'ALL',
			'except_countries' => 'Japan',
			'quote' => false,
			'in_stock' => false,
			'featured' => false,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'auto-premium-bisulfite-kit-40-rxns',
			'meta_title' => 'Auto Premium Bisulfite kit',
			'meta_keywords' => '',
			'meta_description' => 'Auto Premium Bisulfite kit',
			'modified' => '2019-12-16 10:05:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1894',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (24 rxns) - replaced by Premium RRBS kit v2 C02030036',
			'description' => '<div class="extra-spaced"><center></center>The kit Premium RRBS (24 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-V2-x24">Premium RRBS v2 (24 rxns)</a><span>.</span>&nbsp;</div>
<div class="extra-spaced">For the information about the format 96 rxns - check out <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-x96-96-rxns">Premium RRBS (96 rxns)</a>.&nbsp;</div>
<div class="extra-spaced"></div>
<p style="text-align: justify;"></p>',
			'label1' => '',
			'info1' => '',
			'label2' => 'Nature Methods',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard  c1"><a href="#auth-1" class="name"><span class="fn">Anne-Cl&eacute;mence Veillard</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
</ul>
<p>Nature Methods 13 (2016)</p>
<p>Published online <time datetime="2016-01-28">28 January 2016 </time></p>
<p></p>
<p><a href="http://www.nature.com/nmeth/journal/v13/n2/full/nmeth.f.391.html" class="alert button"><span style="font-weight: 400;">CHECK OUT THE NATURE METHODS PAPER</span></a></p>',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C02030032',
			'old_catalog_number' => '',
			'sf_code' => 'C02030032-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '1560',
			'price_USD' => '1640',
			'price_GBP' => '1445',
			'price_JPY' => '272000',
			'price_CNY' => '',
			'price_AUD' => '4100',
			'country' => 'ALL',
			'except_countries' => 'None',
			'quote' => false,
			'in_stock' => true,
			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '0000-00-00',
			'slug' => 'premium-rrbs-kit-x24-24-rxns',
			'meta_title' => 'Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit x24 | Diagenode',
			'meta_keywords' => 'DNA methylation,methylation epigenetics methylation Bisulfite conversion Bisulfite-seq Bis-seq RRBS Reduced Representation Bisulfite Sequencing reduced representation bisulfite DNA methylation sequencing 5-mC 5-methylcytosine',
			'meta_description' => 'Premium RRBS kit analyzes DNA methylation at the single nucleotide level for 4 million CpGs.Reduced Representation Bisulfite Sequencing is fast and affordable.',
			'modified' => '2022-06-02 14:31:26',
			'created' => '2015-06-29 14:08:20',
			'ProductsRelated' => array(
				[maximum depth reached]
			),
			'Image' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1895',
			'antibody_id' => null,
			'name' => 'Premium RRBS kit (96 rxns) - replaced by Premium RRBS v2 x96 C02030037',
			'description' => '<div class="extra-spaced"><span>The kit Premium RRBS (96 rxns) has been replaced by an upgraded version: <a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a></span><span>. Please note, that the adapters are not included in the kit and must be purchased separately. For more information please check out:&nbsp;&nbsp;<a href="https://www.diagenode.com/en/p/premium-rrbs-kit-v2-x96">Premium RRBS v2 (96 rxns)</a>.</span></div>
<div class="extra-spaced"><center></center></div>
<p></p>',
			'label1' => 'Characteristics',
			'info1' => '<ul class="accordion" data-accordion="">
<li><strong>Superior coverage</strong> &ndash; 4 million CpGs</li>
<li><strong>High throughput</strong> - 96 samples processed in one experiment</li>
<li><strong>Cost-efficient</strong> &ndash; Multiplex up to 6 samples/sequencing lane</li>
<li><strong>Validated</strong> with FFPE, cancer, and low-input samples</li>
<li><strong>High efficiency and minimal bias</strong> - Low DNA degradation and reduced amplification</li>
<li><strong>Complete kit</strong> &ndash; Bisulfite conversion reagents, MspI enzyme, library preparation reagents including barcodes, and spike-in controls</li>
<li class="accordion-navigation" style="list-style-type: circle; display: list-item;"><strong>Already tested on various species </strong>&ndash; human, mouse, rat, pig, cow, dog, zebrafish, Daphnia <a href="#species" style="color: #13b29c; background-color: transparent; display: inline; padding: 0;">and more</a> <span class="content" id="species">cichlid fish (Astatotilapia calliptera), mossy frog, yellow-bellied slider, dice snake, zebra finch, humboldt penguin, leaf bird, buzzard, vulturine guinea fowe, parma kangaroo, cheetah, mouflon</span></li>
</ul>
<p>&nbsp;</p>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-1.png" alt="Chr Shearing" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns"><br />
<p><b>Superior&nbsp;coverage</b></p>
<p>Comparison of CpG coverage between competing technologies.</p>
<p><strong><em><small>They love it! </small></em></strong><br /><em><small>The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples. <br /><strong>Paul Datlinger and Christoph Bock, </strong><strong>CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria </strong></small></em></p>
<p><em>&nbsp;</em></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/kits/rrbs-figure-2.jpg" alt="dna methylation" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><b>Accurate determination of DNA methylation level</b></p>
Excellent results were obtained using Diagenode&rsquo;s Premium RRBS kit: almost 90% alignment rate, 4.1 million CpGs covered and bisulfite conversion rates around 99.5% for all samples. DNA methylation percentages in the region of IGF2 obtained with the Diagenode&rsquo;s Premium RRBS Kit.&nbsp;Two human cell lines were analyzed: Gm12878 and MCF7. The MCF7 cell line was studied in duplicates. Each peak represents the DNA methylation percentage at one CpG. The methylated CpGs are shown in red and the unmethylated CpGs in grey.
<p><br /><br /></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/landing-pages/rrbs_how_it_works.jpg" alt="rrbs how it works" style="display: block; margin-left: auto; margin-right: auto;" /><br /><br /></p>
</div>
<div class="small-6 columns">
<p><b>How it works</b></p>
By cutting the genome using the <strong>restriction enzyme MspI</strong> (CCGG target sites) followed by size selection, DNA is enriched to represent <strong>CpG-rich genomic regions</strong> (including CpG islands, CpG island shores, enhancers, and other gene-regulatory elements), which are particularly relevant for epigenetic regulation. Similar to exome-sequencing for mutation discovery, the RRBS protocol enriches for some of the most interesting target regions and thereby achieves a reduction in sequencing cost of a factor of 10-20 compared to whole genome bisulfite sequencing.</div>
</div>
<table width="1039">
<tbody>
<tr></tr>
<tr>
<td width="281">&nbsp;</td>
<td width="379">
<h4>Premium RRBS Kit</h4>
</td>
<td width="379">
<h5>Illumina&reg; RRBS protocol</h5>
</td>
</tr>
<tr>
<td><strong>DNA input</strong></td>
<td>100 ng</td>
<td>2-5 &micro;g</td>
</tr>
<tr>
<td><strong>Multiplexing</strong></td>
<td>Pooling of 6 samples (one HiSeq lane)</td>
<td>no guidelines</td>
</tr>
<tr>
<td><strong>Bisulfite conversion</strong></td>
<td>One bisulfite reaction per pool (6 samples)</td>
<td>One bisulfite reaction per sample</td>
</tr>
<tr>
<td><strong># purification steps</strong></td>
<td>2</td>
<td>7</td>
</tr>
<tr>
<td><strong>Key features</strong></td>
<td width="379">low input - cost-effective - optimized for high-throughput - complete kit</td>
<td width="379">high input - not optimized for high-throughput - reagents from different providers</td>
</tr>
</tbody>
</table>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label2' => 'NATURE METHODS',
			'info2' => '<p><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">NATURE METHODS</span></p>
<p></p>
<h1 class="advertising-feature"><strong>Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage</strong></h1>
<ul class="citation dates">
<li style="text-align: left;" class="vcard last-author"><a href="#auth-5" class="name"><span class="fn">Christoph Bock</span></a><sup><a href="#a2">2</a></sup><sup href="#affil-auth">, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-4" class="name"><span class="fn">Sharon Squazzo</span></a><sup><a href="#a3">3</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-3" class="name"><span class="fn">Miklos Laczik</span></a><sup><a href="#a1">1</a></sup><sup>, </sup></li>
<li style="text-align: left;" class="vcard"><a href="#auth-2" class="name"><span class="fn">Paul Datlinger</span></a><sup><a href="#a2">2</a></sup><sup>, </sup></li&

Premium WGBS kit MANUAL Whole Genome Bisulfite Sequencing (WGBS) is a single nucleotide resolution technique that a...	Download
DNA Methylation Solutions BROCHURE Complete solution for DNA methylation studies! Whether you are experienced or new to th...	Download

Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS GB en	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS US en	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE nl	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS BE fr	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS FR fr	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS ES es	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS DE de	Download
Premium Whole Genome Bisulfite Sequencing (WGBS) kit SDS JP ja	Download

Premium Whole Genome Bisulfite Sequencing (WGBS) kit

Characteristics

How to properly cite this product in your work

Premium Bisulfite kit

Auto Premium Bisulfite kit

Premium RRBS kit (24 rxns) - replaced by Premiu...

Premium RRBS kit (96 rxns) - replaced by Premiu...

Premium WGBS Indexes - 6

Premium WGBS Indexes - 12

Premium WGBS Indexes - 24

RRBS Service (Reduced Representation Bisulfite ...

Bisulfite conversion reagent for RRBS