The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your non-plant MeDIP experiments (Methylated DNA Immunoprecipitation) carried out with Diagenode’s MagMeDIP qPCR and Auto MagMeDIP qPCR Kit. These controls are made from A. thaliana BAC.
CAUTION : However these controls are compatible with (Auto) MagMeDIP qPCR Kit, these are not the same as the ones directly provided with the kit. Please always use controls with their respective primers.
Diagenode strongly recommends using this: DNA Methylation control package (Diagenode Cat# C02040012). Click here to copy to clipboard.
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Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA. Shen SY, Burgener JM, Bratman SV, De Carvalho DD Circulating cell-free DNA (cfDNA) comprises small DNA fragments derived from normal and tumor tissue that are released into the bloodstream. Recently, methylation profiling of cfDNA as a liquid biopsy tool has been gaining prominence due to the presence of tissue-specific markers in cfDNA. We have previously reporte...
DNA methylation and genetic degeneration of the Y chromosome in the dioecious plant Silene latifolia. Rodríguez Lorenzo JL, Hobza R, Vyskot B BACKGROUND: S. latifolia is a model organism for the study of sex chromosome evolution in plants. Its sex chromosomes include large regions in which recombination became gradually suppressed. The regions tend to expand over time resulting in the formation of evolutionary strata. Non-recombination and later accumulat...
Protection of CpG islands from DNA methylation is DNA-encoded and evolutionarily conserved Long HK, King HW, Patient RK, Odom DT, Klose RJ DNA methylation is a repressive epigenetic modification that covers vertebrate genomes. Regions known as CpG islands (CGIs), which are refractory to DNA methylation, are often associated with gene promoters and play central roles in gene regulation. Yet how CGIs in their normal genomic context evade the DNA methylat...
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