Diagenode

iDeal Library Preparation Kit x24 (incl. Index Primer Set 1)

Catalog Number
Format
Price
C05010020
24 rxns
$925.00

The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-generation sequencing, with input amounts down to 5 ng. Our kit offers a simple and fast workflow, high yields, and ready-to-sequence DNA on the Illumina platform. The kit is highly recommended for high input ChIP-seq library preparation, MeDIP-seq library preparation, genomic DNA sequencing. For library preparation of DNA inputs down to 50 pg, our MicroPlex Library Preparation Kit is an optimal choice.

The kit iDeal Library Preparation contains 12 sigle indexes (Set 1). Additional set of 12 single indexes is available separately (Index Primer Set 2, Cat. No. C05010021).

  • Characteristics

    High library yields

    High library yields
    5 ng of input DNA were used for each library prep. A. E. coli genomic DNA libraries. 1: Ladder; 2: Library prepared using iDeal Library Preparation™ Kit.
    B. FFPE sample. Human lung tumor genomic DNA 1: Library prepared using the iDeal Library Preparation™ Kit). 2: Input DNA (degraded FFPE genomic DNA).

    How it works

    iDeal Library preparation kit: how it works

  •  Protocols
    iDeal Library Preparation kit - IP-Star® compact
    The “iDeal Library Preparation” protocol on the IP-Star® is using the standard &l...
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  •  Documents
    iDeal Library Preparation kit x24 (incl. Index Primer Set 1) MANUAL
    The iDeal Library Preparation Kit reliably converts DNA into indexed libraries for next-g...
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    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: iDeal Library Preparation Kit x24 (incl. Index Primer Set 1) (Diagenode Cat# C05010020). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The Wnt-Driven Mll1 Epigenome Regulates Salivary Gland and Head and Neck Cancer.
    Zhu Q, Fang L, Heuberger J, Kranz A, Schipper J, Scheckenbach K, Vidal RO, Sunaga-Franze DY, Müller M, Wulf-Goldenberg A, Sauer S, Birchmeier W
    We identified a regulatory system that acts downstream of Wnt/β-catenin signaling in salivary gland and head and neck carcinomas. We show in a mouse tumor model of K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations that tumor-propagating cells exhibit increased Mll1 activi...

    Genome-wide measurement of local nucleosome array regularity and spacing by nanopore sequencing.
    Baldi S, Krebs S, Blum H, Becker PB
    The nature of chromatin as regular succession of nucleosomes has gained iconic status. However, since most nucleosomes in metazoans are poorly positioned it is unknown to which extent bulk genomic nucleosome repeat length reflects the regularity and spacing of nucleosome arrays at individual loci. We describe a new ...

    MeDIP combined with in-solution targeted enrichment followed by NGS: Inter-individual methylation variability of fetal-specific biomarkers and their implementation in a proof of concept study for NIPT.
    Keravnou A, Ioannides M, Loizides C, Tsangaras K, Achilleos A, Mina P, Kypri E, Hadjidaniel MD, Neofytou M, Kyriacou S, Sismani C, Koumbaris G, Patsalis PC
    DNA methylation is the most characterized epigenetic process exhibiting stochastic variation across different tissues and individuals. In non-invasive prenatal testing (NIPT) fetal specific methylated regions can potentially be used as biomarkers for the accurate detection of fetal aneuploidies. The aim of this stud...

    Chromosome contacts in activated T cells identify autoimmune disease candidate genes
    Burren OS et al.
    BACKGROUND: Autoimmune disease-associated variants are preferentially found in regulatory regions in immune cells, particularly CD4+ T cells. Linking such regulatory regions to gene promoters in disease-relevant cell contexts facilitates identification of candidate disease genes. RESULTS: Within 4 h, act...

    DNA Replication Is Required for Circadian Clock Function by Regulating Rhythmic Nucleosome Composition
    Liu X. et al.
    Although the coupling between circadian and cell cycles allows circadian clocks to gate cell division and DNA replication in many organisms, circadian clocks were thought to function independently of cell cycle. Here, we show that DNA replication is required for circadian clock function in Neurospora. Genetic and ph...

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