Diagenode

Kits

Exosomes

New! Pure and high quality exosome isolationthe perfect solution

Diagenode offers the best tools to isolate and enrich exosomes from diverse biological fluid samples with zero contamination from vesicules or aggregates. Now, with Diagenode’s standardized and high quality exosome isolation solution, you no longer need to compromise exosome isolation. Based on immunocapture, our ExoIP kits offer the most effective and highly specific exosome isolation.

ExoIP Kit - Benefits

  • Highly pure exosome population: ideal for RNA and non-coding RNA analysis (microRNA) protein analysis, Western blot, ELISA, flow cytometry, and more!
  • Ready to use, high-quality: includes antibody-conjugated beads and optimized BSA-free buffers
  • Sample diversity: serum, plasma, and cell culture supernatant
  • Highly validated with multiple methods: Western blot, electron microscopy, RT-qPCR, dynamic light scattering, and flow cytometry

Validation data

Validation by dynamic light scattering

Validation by electron microscopy

Characterization of vesicle population according their overall size by dynamic light scattering (from a Zetasizer Nano S, Malvern Instruments).
Diagenode’s ExoIP yield to highly pure exosome preparations. The measured size of the vesicle population is in perfect accordance with the theoretical size range expected for exosomes: 30-100 nm.


The immunogold anti-CD9 labeling confirms the highly pure exosome preparations from HeLa Cell culture medium by showing clean exosomes in the samples.
The vesicle highlighted herein is identified as an exosome according to their size and it characteristic morphology but also the precise anti-CD9 tag with the gold nanoparticles.


Validation by Western Blot on cell culture medium

Western Blot results show that internal exosomal proteins Alix, HSP70 and TSG101 and membranous exosomal proteins CD63, CD9 and CD81 are expressed in ExoIP isolated vesicules from HEK 293T culture medium. These data demonstrate that vesicles isolated with the Diagenode’s ExoIP kits are very pure exosomes.


Validation by Western Blot on Serum and Plasma




1. CD9 EoxIP kit
2. CD63 ExoIP kit
3. CD81 ExoIP kit
4. Composite ExoIP Kit


Validation by Western Blot: comparison with competitor kits

Western Blot results show that internal exosomal protein HSP70 is highly expressed in exosomes isolated from culture medium of HEK 293T with ExoIP Kits compared to competitor immunocapture bead kits. WCL= whole cell lysate.


Validation by flow cytometry

Flow analysis performed on isolated exosomes from HEK 293T cell culture supernatant with ExoIP CD9 kit (BD FACS Canto II). The amount of input (ml) and beads are equal in each tested condition.

Flow analysis performed on isolated exosomes from different volumes of HEK 293T cell culture supernatant with ExoIP CD81 kit (BD FACS Canto II). Significant signal is obtained from low sample input. Signal is increasing according to the starting volume, reflecting the important binding capacity.

Example of downstream application data

qPCR application analysis to detect miRNA

Relative miR-21 mRNA expression detected by qPCR on plasma exosomes isolated with ExoIP kits. No detection by ultracentrifugation exosome isolation.

Products

Cat. No.ProductFormatPrice
C28010001 ExoIP Composite Kit
ExoIP Composite Kit consists of immunoprecipitation method using a mixture of magnetic beads coupled with antibodies that recognize CD9, CD63, CD81 and ...
$490.00
C28010003 ExoIP CD63 Kit
ExoIP CD63 kit consists of immunoprecipitation method using magnetic capture beads coupled with antibodies that recognize exosome surface antigen CD63 f...
$490.00
C28010004 ExoIP CD81 Kit
ExoIP CD81 kit consists of immunoprecipitation method using magnetic capture beads coupled with antibodies that recognize exosome surface antigen CD81 for an...
$490.00
C28010002 ExoIP CD9 Kit
ExoIP CD9 kit consists of immunoprecipitation method using magnetic capture beads coupled with antibodies that recognize exosome surface antigen CD9 for an o...
$490.00
C28010005 ExoIP EpCAM Kit
ExoIP EpCAM kit consists of immunoprecipitation method using magnetic capture beads coupled with antibodies that recognize exosome antigen EpCAM fo...
$490.00

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
 See all events

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