Diagenode

Diagenode’s  new RNA-sequencing solutions utilize the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5-ends for cDNA synthesis. The Diagenode kits generate ready-to-sequence libraries from RNA inputs as low as 10 picograms in just few hours. Unlike competing solutions, our CATS RNA-seq  kits exhibit higher library efficiency versus ligation-based methods. The CATS method also allows for accurate identification of a greater number of non-coding RNAs, providing higher complexity from better template capture and minimal bias due to lower amplification requirements.

  • Ligation-free  assay - increased efficiency and minimum bias
  • Libraries ready to sequence in only 5 hours
  • User-friendly: reduced hands-on  time with fewer steps than competing methods
  • Low inputs down to 10 picograms
  • Retaining strand specificity of origin

 

How to choose your RNA-seq library kit?

Note: input refers to isolated small RNA, rRNA depleted or poly(A) selected RNA

CATS Small RNA seq-Kit CATS Total RNA seq-Kit CATS RNA seq-Kit CATS mRNA seq-Kit

CATS for Small RNA White Paper:

Low input RNA-seq library preparation provides higher small non-coding RNA diversity and greatly reduced hands-on time

Prior to RNA sequencing, the preparation of suitable libraries for the NGS platform is required . The library preparation typically requires the conversion of the template RNA into cDNA and the fusion of adapters to both ends before PCR amplification.For ligation-based techniques, the addition of the adapters by RNA ligase is heavily biased because of its uneven affinity towards the different template sequences. In addition, these techniques necessitate large amounts of RNA input.

This white paper discusses the straightforward, cost-effective, highly efficient “Capture and Amplification by Tailing and Switching” (CATS) to easily generate ready-to-sequence NGS libraries from picogram quantities of RNA.

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Products

Cat. No.ProductFormatPrice
C05010043-24 CATS mRNA-seq Kit v2 (with polyA selection)
Diagenode’s CATS mRNA-seq Kit (with polyA selection) utilizes the innovative “capture and amplification by tailing and switching” (CATS), a...
24 rxns $1,395.00
C05010040-24 CATS Small RNA-seq Kit
Diagenode’s CATS Small RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free met...
24 rxns $1,360.00
C05010042-24 CATS Total RNA-seq Kit v2 (with rRNA depletion)
Diagenode’s CATS Total RNA-seq Kit (with rRNA depletion) utilizes the innovative “capture and amplification by tailing and switching” (CATS...
24 rxns $2,450.00
C05010041-24 CATS RNA-seq Kit v2
Diagenode’s CATS RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free method to...
24 rxns $1,360.00

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    Copenhagen, Denmark
    May 27-May 30, 2017
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