Diagenode

NGS Library preparation

RNA library preparation

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This technology offers a new RNA-sequencing solution, optimized and specifically formatted for both Illumina and MGI sequencing platforms.

Using D-Plex technology is optimal for studying non-coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. Library preparation enables diverse and novel transcript detection, ensuring realistic representation of the transcriptome with minimized bias. The unique molecular identifier (UMI) sequence enables accurate bioinformatic identification and removal of PCR duplicates from amplified libraries.

Our new D-Plex RNA-seq kits provide streamlined workflows with easy-to-follow protocols. The library preparation takes place in a single tube, greatly increasing efficiency. This ensures high technical reliability and reproducibility. Our D-Plex RNA-seq kits have been extensively validated for clinically relevant samples such as liquid biopsy samples. The D-Plex technology is optimal for epigenetic biomarker discovery.

Why the new D-Plex technology benefit RNA research?

  • Ultrasensitive, ligation-free RNA-seq library preparation
  • Ultra-low input requirements: down to 10 pg for small RNAs, 100 pg for total RNAs
  • High library complexity enabling detection of novel and diverse transcripts
  • Improved quantification accuracy using UMIs
  • Optimal performance on clinical samples, such as liquid biopsies
  • One-day, one-tube workflow with minimal hands-on time
  • Compatible with both Illumina and MGI sequencing platforms

“We work with extremely limited quantities of small RNAs. In our hands, D-Plex had the highest yield compared to many other approaches. Inclusion of UMIs is essential for our small RNA analysis”

Can Cenik, PhD
The University of Texas at Austin

Cell-Free transcriptome

Cat. No.ProductFormatPrice
C05030040 D-Plex cell-free RNA-seq Kit
Full-spectrum extracellular RNA profiling for non-invasive biomarker discovery Cell-free RNA (cfRNA) in biofluids is an exceptionally dynamic and informatio...
24 rxns $1,545.00
C05030045 D-Plex UDI for cell-free RNA-seq - Set A
D-Plex UDI for cell-free RNA-seq - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexing with the D-Pl...
24 rxns $375.00
C05030046 D-Plex UDI for cell-free RNA-seq - Set B
D-Plex UDI for cell-free RNA-seq - Set B includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexing with the...
24 rxns $375.00

Small non-coding transcriptome - Illumina sequencing platform

Cat. No.ProductFormatPrice
C05030001 D-Plex Small RNA-seq Kit for Illumina
Product Features Ultra-low input capability, down to 10 pg for small RNAs and 100 pg for total RNA samples High library complexity providing novel and ...
24 rxns $1,530.00
C05030021 D-Plex Unique Dual Indexes for Illumina - Set A
D-Plex Unique Dual Indexes Module - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiple...
24 rxns $375.00
C05030022 D-Plex Unique Dual Indexes for Illumina - Set B
D-Plex Unique Dual Indexes Module - Set B includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiple...
24 rxns $375.00
C05030023 D-Plex Unique Dual Indexes for Illumina - Set C
D-Plex Unique Dual Indexes Module - Set C includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiple...
24 rxns $375.00
C05030024 D-Plex Unique Dual Indexes for Illumina - Set D
D-Plex Unique Dual Indexes Module - Set D includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiple...
24 rxns $375.00

Small non-coding transcriptome - MGI sequencing platform

Cat. No.ProductFormatPrice
C05030060 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set A
D-Plex DNBSEQ Barcodes - Set A includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA DNBSEQTM Kit. ...
24 rxns $360.00
C05030061 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B
D-Plex DNBSEQ Barcodes - Set B includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA...
24 rxns $360.00
C05030051 D-Plex Small RNA DNBSEQ™ Kit for MGI Sequencing
Download the manual D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-...
24 rxns $1,555.00

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