Diagenode

Library preparation

Library preparation for RNA-seq

Diagenode’s  new RNA-sequencing solutions utilize the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to produce DNA libraries for next generation sequencing from low input amounts of RNA. CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5-ends for cDNA synthesis. The Diagenode kits generate ready-to-sequence libraries from RNA inputs as low as 10 picograms in just few hours. Unlike competing solutions, our CATS RNA-seq  kits exhibit higher library efficiency versus ligation-based methods. The CATS method also allows for accurate identification of a greater number of non-coding RNAs, providing higher complexity from better template capture and minimal bias due to lower amplification requirements.

  • Ligation-free  assay - increased efficiency and minimum bias
  • Libraries ready to sequence in only 5 hours
  • User-friendly: reduced hands-on  time with fewer steps than competing methods
  • Low inputs down to 10 picograms
  • Retaining strand specificity of origin

 

How to choose your RNA-seq library kit?

Note: input refers to isolated small RNA, rRNA depleted or poly(A) selected RNA

CATS Small RNA seq-Kit CATS Total RNA seq-Kit CATS RNA seq-Kit CATS mRNA seq-Kit

Products

Cat. No.ProductFormatPrice
C05010043 CATS mRNA-seq Kit (with polyA selection)
Diagenode’s CATS mRNA-seq Kit (with polyA selection) utilizes the innovative “capture and amplification by tailing and switching” (CATS), a...
24 rxns $1,395.00
C05010040 CATS Small RNA-seq Kit
Diagenode’s CATS Small RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free met...
24 rxns $1,360.00
C05010042 CATS Total RNA-seq Kit (with rRNA depletion)
Diagenode’s CATS Total RNA-seq Kit (with rRNA depletion) utilizes the innovative “capture and amplification by tailing and switching” (CATS...
24 rxns $2,450.00
C05010041 CATS RNA-seq Kit
Diagenode’s CATS RNA-seq Kit utilizes the innovative “capture and amplification by tailing and switching” (CATS), a ligation-free method to...
24 rxns $1,360.00

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