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- Ultra-low input capability, down to 10 pg for small RNAs or circulating RNAs
- Higher library complexity providing novel and diverse transcript detection
- Improved quantification accuracy through the use of UMIs
- Optimal performance on liquid biopsy samples such as plasma, serum, and urine
- Easy to use with minimal hands-on time: one day, one tube protocol
Note: The D-Plex Small RNA-seq Kit manual describes now the protocol for both UDI and SI library constructions.
D-Plex Small RNA-seq Library Prep Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-Plex technology to generate small RNA libraries for Illumina sequencing directly from total RNAs or enriched small RNAs.
The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. Combined with unique molecular identifiers (UMI), this complete solution ensures a realistic and accurate representation of diverse small RNA species (such as microRNAs, piRNAs, tRNAs, and siRNAs) with minimized quantitative bias.
D-Plex Small RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been extensively validated for liquid biopsy, such as from human plasma, making it an important tool for clinically-relevant studies.
D-Plex Small RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Indexes were designed and validated to fit the D-Plex technology and are available separately:
For D-Plex UDI library construction:
For D-Plex SI library construction:
D-Plex is also available for MGI sequencing, check here!
Focused on biomarker research?
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One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.
With a robust technology
Independent of your input amount -- go down to 10 pg small RNA as a starting amount.
And raise your quality output
Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.
Need help for your bioinformatics analysis of miRNA?
Diagenode has collaborated with Andreas Keller and Tobias Fehlmann to develop a new bioinformatics pipeline for the prediction of novel miRNAs.
Interested in exosome research?
Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). Discover our exosome isolation strategy here!