D-Plex Small RNA-seq Kit x24 for Illumina

Diagenode’s latest innovation in RNA-seq, D-Plex, is based on a template-switching technology that delivers a ligation-free method for RNA library preparation, ensuring realistic representation of diverse small RNA species (such as miRNA, piRNA, tRNA, and siRNA) with minimized bias. This solution allows you to produce Illumina®-compatible DNA libraries for NGS from low input amounts of RNA with minimized PhiX spike-in quantity for the most representative results.

The D-Plex small RNA solution was optimized for 10 pg - 10 ng enriched small RNA (or 100 pg - 100 ng total RNA) and contains our latest TSO (Template-Switch Oligonucleotides) technology including UMIs. The kit is provided with our new bioinformatics tool especially developed in parallel to get the best of your data.

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  • Truest representation - UMIs with ligation-free protocol ensures the most representative and unbiased data
  • Easy - One-tube, one-day protocol Lowest inputs - Down to 10 pg enriched small RNA or 100 pg total RNA starting amount
  • Clinical sample compatibility - Tested with RNA from biofluids
  • Highest sequencing quality - Minimized PhiX quantity
  • Enhanced data - Our new bioinformatics pipeline provided in the manual was optimized and developed specifically for getting the best out of your D-Plex data including UMI processing

  miRMaster tool for miRNA

  

  Diagenode has collaborated with Andreas Keller and Tobias Fehlmann to develop a new bioinformatics pipeline in order to allow novel miRNA prediction.
  miRMaster is a comprehensive analysis online free tool for NGS miRNA samples.

Focused on your target

D-Plex: the highest yield technology to detect every kind of small RNA simultaneously (miRNA, snRNA, piRNA, …)

Get rich content

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

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