D-Plex Small RNA-seq Kit for Illumina

D-Plex Small RNA-seq Library Prep Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-Plex technology to generate small RNA libraries for Illumina sequencing directly from total RNA or enriched small RNA.

The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low inputs, down to 10 pg for small RNA and 100 pg for total RNA. Combined with UMIs, this complete solution ensures a realistic and accurate representation of diverse small RNA species (such as miRNA, piRNA, tRNA, and siRNA) with minimized quantitative bias.

D-Plex Small RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. This new solution has been extensively validated for liquid biopsy, such as from human plasma, making it an important tool for clinically-relevant studies.

D-Plex Small RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Single Indexes were designed and validated to fit this technology and are available separately:

• C05030010 - D-Plex Single Indexes for Illumina - Set A
• C05030011 - D-Plex Single Indexes for Illumina - Set B

Read more about the D-Plex technology or discover the D-Plex Kit compatible with MGI sequencing.

Focused on your target

Catch all kind of small non-coding RNAs with D-Plex Small RNA-seq Kit.

Get rich content

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

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