Diagenode

D-Plex Small RNA-seq Kit x24 for Illumina

Catalog Number
Format
Price
C05030001
24 rxns
$1,295.00

D-Plex Small RNA-seq library prep kit for Illumina

Diagenode’s latest innovation in RNA-seq, D-Plex, is based on a template-switching technology that delivers a ligation-free method for RNA library preparation, ensuring realistic representation of diverse small RNA species (such as miRNA, piRNA, tRNA, and siRNA) with minimized bias. This solution allows you to produce Illumina®-compatible DNA libraries for NGS from low input amounts of RNA with minimized PhiX spike-in quantity for the most representative results.

The D-Plex small RNA solution was optimized for 10 pg - 10 ng enriched small RNA (or 100 pg - 100 ng total RNA) and contains our latest TSO (Template-Switch Oligonucleotides) technology including UMIs. The kit is provided with our new bioinformatics tool especially developed in parallel to get the best of your data.

  • Read more
    • Truest representation - UMIs with ligation-free protocol ensures the most representative and unbiased data
    • Easy - One-tube, one-day protocol Lowest inputs - Down to 10 pg enriched small RNA or 100 pg total RNA starting amount
    • Clinical sample compatibility - Tested with RNA from biofluids
    • Highest sequencing quality - Minimized PhiX quantity
    • Enhanced data - Our new bioinformatics pipeline provided in the manual was optimized and developed specifically for getting the best out of your D-Plex data including UMI processing

    miRMaster tool for miRNA

    Diagenode has collaborated with Andreas Keller and Tobias Fehlmann to develop a new bioinformatics pipeline in order to allow novel miRNA prediction.
    miRMaster is a comprehensive analysis online free tool for NGS miRNA samples.

Download the manual

Focused on your target

small RNA sequencing kit

D-Plex: the highest yield technology to detect every kind of small RNA simultaneously (miRNA, snRNA, piRNA, …)

Get rich content

small RNA diversity

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

small non coding RNA

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

small RNA library preparation for Illumina

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

[ To check figures in details, click on "Figure" in the menu below ]

  • Indexes

    Indexes are not included in the kit, you can choose between two compatible sets of 24 Single Indexes (SI):

  • Data analysis

    Bioinformatics guidelines

    A specific pipeline and script have been developed with the integration of UMI and our template-switch oligo. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex construct, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  • Figures

    Small non-coding RNAs

    Figure 1. Catch all kind of small non coding RNAs with D-Plex Small RNA-seq Kit.

    Biotyping

    Figure 2. Biotyping. Relative abundance of each small RNA type captured in the library out of the total amount of small RNA. (RNA transcripts annotated in Ensembl).

    Low inputs correlation

    Figure 3. Correlation between 2 different starting amounts using D-Plex Small RNA-seq Kit. 10 ng and 10 pg of small RNA were isolated for ncRNA detection at TPM ≥2. r = 0.97 (red line). Ideal correlation is represented by the blue line.

    Sequencing output quality

    Figure 4. D-Plex validated on NovaSeq system (100 nt SE reads) using different sample types.
    (A)
    1 ng RNA isolated from rat plasma.
    (B)
    100 pg RNA isolated from human serum.

  •  Documents
    D-Plex Small RNA-seq Kit - User manual MANUAL
    Get the small RNA-sequencing user manual to know more about the D-Plex protocol by click...
    Download
    D-Plex Small RNA-seq library preparation solution FLYER
    True and accurate RNA-seq: Utilize the power of UMIs without ligation
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Small RNA-seq Kit x24 for Illumina (Diagenode Cat# C05030001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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