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D-Plex Small RNA-seq Kit for Illumina




Catalog Number
Format
Price
C05030001
24 rxns
$1,295.00

Small RNA-seq library prep kit for Illumina

Download the manual

Note: The D-Plex Small RNA-seq Kit manual describes now the protocol for both UDI and SI library constructions.

D-Plex Small RNA-seq Library Prep Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-Plex technology to generate small RNA libraries for Illumina sequencing directly from total RNAs or enriched small RNAs.

The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. Combined with unique molecular identifiers (UMI), this complete solution ensures a realistic and accurate representation of diverse small RNA species (such as microRNAs, piRNAs, tRNAs, and siRNAs) with minimized quantitative bias.

D-Plex Small RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been extensively validated for liquid biopsy, such as from human plasma, making it an important tool for clinically-relevant studies.

D-Plex Small RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Indexes were designed and validated to fit the D-Plex technology and are available separately:

For D-Plex UDI library construction:

For D-Plex SI library construction:

D-Plex is also available for MGI sequencing, check here!

Focused on your target

small RNA sequencing kit

Catch all kind of small non-coding RNAs with D-Plex Small RNA-seq Kit.

Get rich content

small RNA diversity

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

small non coding RNA

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

small RNA library preparation for Illumina

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

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  • Product features
    • Truest representation: a ligation-free protocol with UMI ensures the most representative and unbiased data
    • Ultra-low input capability: down to 10 pg for small RNAs and 100 pg for total RNAs
    • Higher library complexity: obtain a complete view of your small RNA transcriptome
    • Optimal performance on clinical samples: validated with circulating RNAs from liquid biopsies
    • Easy to use with minimal hands-on time: one day, one tube protocol
    • Highest sequencing quality: requires minimized PhiX spike-in quantity for Illumina sequencing

  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    For D-Plex UDI library construction:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

    For D-Plex SI library construction:

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  Documents
    D-Plex Small RNA-seq Kit - User manual MANUAL
    Get the small RNA-sequencing user manual to know more about the D-Plex protocol by click...
    Download
    D-Plex Small RNA-seq library preparation solution FLYER
    True and accurate RNA-seq: Utilize the power of UMIs without ligation
    Download
  •  Safety sheets
    D-Plex Small RNA-seq Kit x24 for Illumina SDS US en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS GB en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS ES es Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS DE de Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS JP ja Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE nl Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE fr Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS FR fr Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Small RNA-seq Kit for Illumina (Diagenode Cat# C05030001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Genes with 5′ terminal oligopyrimidine tracts preferentially escapeglobal suppression of translation by the SARS-CoV-2 NSP1 protein
    Shilpa, Rao and Ian, Hoskins and P., Daniela Garcia and Tori, Tonn andHakan, Ozadam and Elif, Sarinay Cenik and ProfileCan, Cenik
    Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, NSP1, for shutting down host translation. Despite this, it is currently unknown how viral pr...

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