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D-Plex Small RNA-seq Kit for Illumina




Catalog Number
Format
Price
C05030001
24 rxns
$1,295.00

Product Features

  • Ultra-low input capability, down to 10 pg for small RNAs or circulating RNAs
  • Higher library complexity providing novel and diverse transcript detection
  • Improved quantification accuracy through the use of UMIs
  • Optimal performance on liquid biopsy samples such as plasma, serum, and urine
  • Easy to use with minimal hands-on time: one day, one tube protocol

Download the manual

Note: The D-Plex Small RNA-seq Kit manual describes now the protocol for both UDI and SI library constructions.

D-Plex Small RNA-seq Library Prep Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-Plex technology to generate small RNA libraries for Illumina sequencing directly from total RNAs or enriched small RNAs.

The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. Combined with unique molecular identifiers (UMI), this complete solution ensures a realistic and accurate representation of diverse small RNA species (such as microRNAs, piRNAs, tRNAs, and siRNAs) with minimized quantitative bias.

D-Plex Small RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been extensively validated for liquid biopsy, such as from human plasma, making it an important tool for clinically-relevant studies.

D-Plex Small RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Indexes were designed and validated to fit the D-Plex technology and are available separately:

For D-Plex UDI library construction:

For D-Plex SI library construction:

D-Plex is also available for MGI sequencing, check here!

Focused on biomarker research?

Get rich content

small RNA diversity

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

With a robust technology

small non coding RNA

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

And raise your quality output

small RNA library preparation for Illumina

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

Need help for your bioinformatics analysis of miRNA?

Diagenode has collaborated with Andreas Keller and Tobias Fehlmann to develop a new bioinformatics pipeline for the prediction of novel miRNAs.

Interested in exosome research? 

Diagenode has developed specific tools for the most efficient exosome capture from biofluids (e.g. plasma, serum, urine, ...). Discover our exosome isolation strategy here!

  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    For D-Plex UDI library construction:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

    For D-Plex SI library construction:

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  Documents
  •  Safety sheets
    D-Plex Small RNA-seq Kit x24 for Illumina SDS US en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS GB en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE fr Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS FR fr Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS ES es Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS DE de Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS JP ja Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE nl Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Small RNA-seq Kit for Illumina (Diagenode Cat# C05030001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Vesicle-bound regulatory RNAs are associated with tissue aging
    F. Kern, T. Kuhn, N. Ludwig, M. Simon, L. Gröger, N. Fabis, A. Salhab, T. Fehlmann, O. Hahn, A. Engel, M. Koch, J. Koehler, K. Winek, H. Soreq, G. Fuhrmann, T. Wyss-Coray, E. Meese, M. W. Laschke and A. Keller
    Previous work on murine models and human demonstrated global as well as tissue-specific molecular aging trajectories in solid tissues and body fluids1–8. Extracellular vesicles like exosomes play a crucial role in communication and information exchange in between such systemic factors and solid tissues9,10. We...

    miRMaster 2.0: multi-species non-coding RNA sequencing analyses at scale
    Tobias Fehlmann, Fabian Kern, Omar Laham, Christina Backes, Jeffrey Solomon, Pascal Hirsch, Carsten Volz, Rolf Müller, Andreas Keller
    Analyzing all features of small non-coding RNA sequencing data can be demanding and challenging. To facilitate this process, we developed miRMaster. After the analysis of over 125 000 human samples and 1.5 trillion human small RNA reads over 4 years, we present miRMaster 2 with a wide range of updates and new featur...

    Genes with 5′ terminal oligopyrimidine tracts preferentially escapeglobal suppression of translation by the SARS-CoV-2 NSP1 protein
    Shilpa, Rao and Ian, Hoskins and P., Daniela Garcia and Tori, Tonn andHakan, Ozadam and Elif, Sarinay Cenik and ProfileCan, Cenik
    Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, NSP1, for shutting down host translation. Despite this, it is currently unknown how viral pr...

    Web-based NGS data analysis using miRMaster: a large-scale meta-analysis of human miRNAs
    Tobias Fehlmann, Christina Backes, Mustafa Kahraman, Jan Haas, Nicole Ludwig, Andreas E. Posch, Maximilian L. Würstle, Matthias Hübenthal, Andre Franke, Benjamin Meder, Eckart Meese, Andreas Keller
    The analysis of small RNA NGS data together with the discovery of new small RNAs is among the foremost challenges in life science. For the analysis of raw high-throughput sequencing data we implemented the fast, accurate and comprehensive web-based tool miRMaster. Our toolbox provides a wide range of modules for qua...

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    Nov 12, 2021
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