Diagenode

D-Plex Small RNA-seq Library Prep Kit x24

カタログ番号
フォーマット
価格
C05030001
24 rxns
$1,295.00

D-Plex Small RNA-seq library prep kit for Illumina

Diagenodeの最新のRNA-seqイノベーションD-Plexは、RNAライブラリー調製の際にライゲーション不要な方法を提供するtemplate-switching技術に基づいています。多様なsmall RNA種(miRNA、piRNA、tRNA、 siRNA)に対応し、バイアスを最小化。 このソリューションにより、NGS用Illumina®互換のDNAライブラリーを低インプット量から作成し、最小限のPhiXスパイクイン量で、最も代表的な結果が期待できます。

D-Plex small RNAソリューションは、10 pg〜10 ngの濃縮small RNA(またはトータルRNA100 pg〜100 ng)向けに最適化されており、UMIを含む最新のTSO(Template-Switch Oligonucleotides)テクノロジーが含まれています。 このキットは、データの最大限活用を目的として、本キットと同時開発された新しいバイオインフォマティクスツールもご覧ください。

Focused on your target

D-Plex: the highest yield technology to detect every kind of small RNA simultaneously (miRNA, snRNA, piRNA, …)

Get rich content

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

[ To check figures in details, click on "Figure" in the menu below ]

  • インデックス

    Indexes are not included in the kit, you can choose between two compatible sets of 24 Single Indexes (SI):

  • データ解析

    Bioinformatics guidelines

    A specific pipeline and script have been developed with the integration of UMI and our template-switch oligo. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex construct, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  • フィギュア

    Small non-coding RNAs

    Figure 1. Catch all kind of small non coding RNAs with D-Plex Small RNA-seq Kit.

    Biotyping

    Figure 2. Biotyping. Relative abundance of each small RNA type captured in the library out of the total amount of small RNA. (RNA transcripts annotated in Ensembl).

    Low inputs correlation

    Figure 3. Correlation between 2 different starting amounts using D-Plex Small RNA-seq Kit. 10 ng and 10 pg of small RNA were isolated for ncRNA detection at TPM ≥2. r = 0.97 (red line). Ideal correlation is represented by the blue line.

    Sequencing output quality

    Figure 4. D-Plex validated on NovaSeq system (100 nt SE reads) using different sample types.
    (A)
    1 ng RNA isolated from rat plasma.
    (B)
    100 pg RNA isolated from human serum.

  •  資料
    D-Plex Small RNA-seq Kit - User manual MANUAL
    Get the small RNA-sequencing user manual to know more about the D-Plex protocol by click...
    Download
    D-Plex Small RNA-seq library preparation solution FLYER
    True and accurate RNA-seq: Utilize the power of UMIs without ligation
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Small RNA-seq Library Prep Kit x24 (Diagenode Cat# C05030001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  関連商品

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  • EpiChrom
    Umea Sweden
    Feb 27-Feb 28, 2020
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