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Pervasive translation of Xrn1-sensitive unstable long non-coding RNAs in yeast


Andjus S. et al.

Despite being predicted to lack coding potential, cytoplasmic long non-coding (lnc)RNAs can associate with ribosomes. However, the landscape and biological relevance of lncRNAs translation remains poorly studied. In yeast, cytoplasmic Xrn1-sensitive lncRNAs (XUTs) are targeted by the Nonsense-Mediated mRNA Decay (NMD), suggesting a translation-dependent degradation process. Here, we report that XUTs are pervasively translated, which impacts their decay. We show that XUTs globally accumulate upon translation elongation inhibition, but not when initial ribosome loading is impaired. Ribo-Seq confirmed ribosomes binding to XUTs and identified actively translated 5'-proximal small ORFs. Mechanistically, the NMD-sensitivity of XUTs mainly depends on the 3'-untranslated region length. Finally, we show that the peptide resulting from the translation of an NMD-sensitive XUT reporter exists in NMD-competent cells. Our work highlights the role of translation in the post-transcriptional metabolism of XUTs. We propose that XUT-derived peptides could be exposed to the natural selection, while NMD restricts XUTs levels.

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D-Plex small RNA-seq

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Published
March, 2024

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  • Small RNA library preparation with UMI for Illumina
    C05030001
    D-Plex Small RNA-seq Library Prep Kit x24
  • Illumina index primers for D-Plex small RNA library preparation with UMI
    C05030021
    D-Plex Unique Dual Indexes for Illumina - Set A

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