Diagenode

D-Plex Total RNA-seq Kit for Illumina




Catalog Number
Format
Price
C05030031
24 rxns
$1,320.00

(Coming soon)

D-Plex Total RNA-seq Library Preparation kit is a tool designed for the study of the whole coding and non-coding transcriptome. The kit is using the D-Plex technology to generate directionnal libraries for Illumina sequencing directly from total RNAs, mRNAs that has already been enriched by poly(A) selection, or RNAs that has already been depleted of rRNAs.

The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts, down to 50 pg for total RNAs, mRNAs or rRNA-depleted RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for low-abundance or rare transcripts from challenging samples.

D-Plex Total RNA-seq Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been extensively validated for both intact and highly degraded RNA samples, including that derived from FFPE preparations.

D-Plex Total RNA-seq Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:

  • Product features
  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    Small RNA seq Bioinformatics pipeline

  •  資料
  •  Safety sheets
    D-Plex Total RNA-seq Kit for Illumina SDS GB en Download
    D-Plex Total RNA-seq Kit for Illumina SDS US en Download
    D-Plex Total RNA-seq Kit for Illumina SDS BE nl Download
    D-Plex Total RNA-seq Kit for Illumina SDS BE fr Download
    D-Plex Total RNA-seq Kit for Illumina SDS FR fr Download
    D-Plex Total RNA-seq Kit for Illumina SDS ES es Download
    D-Plex Total RNA-seq Kit for Illumina SDS DE de Download
    D-Plex Total RNA-seq Kit for Illumina SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Total RNA-seq Kit for Illumina (Diagenode Cat# C05030031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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    Light at night has strong effects on physiology and behavior of mammals. It affects mood in humans, which is exploited as light therapy, and has been shown to reset the circadian clock in the suprachiasmatic nuclei (SCN). This resetting is paramount to align physiological and biochemical timing to the environmental ...

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    Photoreceptor cell death and inflammation are known to occur progressively in retinal degenerative diseases such as age-related macular degeneration (AMD). However, the molecular mechanisms underlying these biological processes are largely unknown. Extracellular vesicles (EV) are essential mediators of cell-to-cell ...

    The ribosomal protein S1-dependent standby site in mRNA consists ofa single-stranded region and a 5' structure element.
    Romilly Cédric et al.
    In bacteria, stable RNA structures that sequester ribosome-binding sites (RBS) impair translation initiation, and thus protein output. In some cases, ribosome standby can overcome inhibition by structure: 30S subunits bind sequence-nonspecifically to a single-stranded region and, on breathing of the inhibitory struc...

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