Diagenode

D-Plex Small RNA-seq Kit x24 for Illumina




目录号
格式
价格
C05030001
24 rxns
$1,320.00

D-Plex Small RNA-seq library prep kit for Illumina

Download the manual

Diagenode’s latest innovation in RNA-seq, D-Plex, is based on a template-switching technology that delivers a ligation-free method for RNA library preparation, ensuring realistic representation of diverse small RNA species (such as miRNA, piRNA, tRNA, and siRNA) with minimized bias. This solution allows you to produce Illumina®-compatible DNA libraries for NGS from low input amounts of RNA with minimized PhiX spike-in quantity for the most representative results.

The D-Plex small RNA solution was optimized for 10 pg - 10 ng enriched small RNA (or 100 pg - 100 ng total RNA) and contains our latest TSO (Template-Switch Oligonucleotides) technology including UMIs. The kit is provided with our new bioinformatics tool especially developed in parallel to get the best of your data.

  • Read more
    • Truest representation - UMIs with ligation-free protocol ensures the most representative and unbiased data
    • Easy - One-tube, one-day protocol Lowest inputs - Down to 10 pg enriched small RNA or 100 pg total RNA starting amount
    • Clinical sample compatibility - Tested with RNA from biofluids
    • Highest sequencing quality - Minimized PhiX quantity
    • Enhanced data - Our new bioinformatics pipeline provided in the manual was optimized and developed specifically for getting the best out of your D-Plex data including UMI processing

    miRMaster : your bioinformatics tool for miRNA

    Diagenode has collaborated with Andreas Keller and Tobias Fehlmann to develop a new bioinformatics pipeline in order to allow novel miRNA prediction.
    miRMaster is a comprehensive analysis online free tool for NGS miRNA samples.

Focused on your target

small RNA sequencing kit

D-Plex: the highest yield technology to detect every kind of small RNA simultaneously (miRNA, snRNA, piRNA, …)

Get rich content

small RNA diversity

One day to enrich your sample with a large variety of small RNAs in one tube. Identify your reads accurately with UMIs.

Robust technology

small non coding RNA

Independent of your input amount -- go down to 10 pg small RNA as a starting amount.

Raise your quality output

small RNA library preparation for Illumina

Get the best of your data -- use our designed bioinformatics pipeline and guidelines. We provide everything you need to greatly simplify your analysis.

[ To check figures in details, click on "Figure" in the menu below ]

  • Indexes

  • Data analysis

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    For D-Plex UDI library construction:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

    For D-Plex SI library construction:

  • Figures

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  文档
  •  Safety sheets
    D-Plex Small RNA-seq Kit x24 for Illumina SDS GB en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS US en Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE nl Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS FR fr Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS BE fr Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS ES es Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS JP ja Download
    D-Plex Small RNA-seq Kit x24 for Illumina SDS DE de Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex Small RNA-seq Kit x24 for Illumina (Diagenode Cat# C05030001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Neutral sphingomyelinase 2 inhibition attenuates extracellular vesicle release and improves neurobehavioral deficits in murine HIV
    Xiaolei Zhu et al.
    People living with HIV (PLH) have significantly higher rates of cognitive impairment (CI) and major depressive disorder (MDD) versus the general population. The enzyme neutral sphingomyelinase 2 (nSMase2) is involved in the biogenesis of ceramide and extracellular vesicl...

    MGcount: a total RNA-seq quantification tool to address multi-mappingand multi-overlapping alignments ambiguity in non-coding transcripts
    Hita Andrea, Brocart Gilles, Fernandez Ana, Rehmsmeier Marc, Alemany Anna, Schvartzman Sol
    Background Total-RNA sequencing (total-RNA-seq) allows the simultaneous study of both the coding and the non-coding transcriptome. Yet, computational pipelines have traditionally focused on particular biotypes, making assumptions that are not fullfilled by total-RNA-seq datasets. Transcripts from distinct RNA biotyp...

    Single cell quantification of ribosome occupancy in early mouse development
    Tori Tonn et al.
    Technological limitations precluded transcriptome-wide analyses of translation at single cell resolution. To solve this challenge, we developed a novel microfluidic isotachophoresis approach, named RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP), and characterized translation in single oocytes and embryos during ...

    Functional microRNA targetome undergoes degeneration-induced shift in the retina
    Chu-Tan JA et al.
    Background: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with argonaute 2 (AGO2) - a key member of the RNA Induced Silencing Complex...

    Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors
    Mørup N. et al.
    Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small RNAs in SP can be used to diagnose men with TGCTs or the precursor le...

    Vesicle-bound regulatory RNAs are associated with tissue aging
    F. Kern, T. Kuhn, N. Ludwig, M. Simon, L. Gröger, N. Fabis, A. Salhab, T. Fehlmann, O. Hahn, A. Engel, M. Koch, J. Koehler, K. Winek, H. Soreq, G. Fuhrmann, T. Wyss-Coray, E. Meese, M. W. Laschke and A. Keller
    Previous work on murine models and human demonstrated global as well as tissue-specific molecular aging trajectories in solid tissues and body fluids1–8. Extracellular vesicles like exosomes play a crucial role in communication and information exchange in between such systemic factors and solid tissues9,10. We...

    miRMaster 2.0: multi-species non-coding RNA sequencing analyses at scale
    Tobias Fehlmann, Fabian Kern, Omar Laham, Christina Backes, Jeffrey Solomon, Pascal Hirsch, Carsten Volz, Rolf Müller, Andreas Keller
    Analyzing all features of small non-coding RNA sequencing data can be demanding and challenging. To facilitate this process, we developed miRMaster. After the analysis of over 125 000 human samples and 1.5 trillion human small RNA reads over 4 years, we present miRMaster 2 with a wide range of updates and new featur...

    Genes with 5′ terminal oligopyrimidine tracts preferentially escapeglobal suppression of translation by the SARS-CoV-2 NSP1 protein
    Shilpa, Rao and Ian, Hoskins and P., Daniela Garcia and Tori, Tonn andHakan, Ozadam and Elif, Sarinay Cenik and ProfileCan, Cenik
    Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, NSP1, for shutting down host translation. Despite this, it is currently unknown how viral pr...

    Repeat RNAs associate with replication forks and post-replicative DNA.
    Gylling HM, Gonzalez-Aguilera C, Smith MA, Kaczorowski DC, Groth A, Lund AH
    Non-coding RNA has a proven ability to direct and regulate chromatin modifications by acting as scaffolds between DNA and histone-modifying complexes. However, it is unknown if ncRNA plays any role in DNA replication and epigenome maintenance, including histone eviction and re-instalment of histone-modifications aft...

    The sncRNA Zoo: a repository for circulating small noncoding RNAs in animals.
    Fehlmann T, Backes C, Pirritano M, Laufer T, Galata V, Kern F, Kahraman M, Gasparoni G, Ludwig N, Lenhof HP, Gregersen HA, Francke R, Meese E, Simon M, Keller A
    The repertoire of small noncoding RNAs (sncRNAs), particularly miRNAs, in animals is considered to be evolutionarily conserved. Studies on sncRNAs are often largely based on homology-based information, relying on genomic sequence similarity and excluding actual expression data. To obtain information on sncRNA expres...

    NGS analysis of total small non coding RNAs from low input RNA from dried blood sampling
    Marcello Pirritano, Tobias Fehlmann, Thomas Laufer, Nicole Ludwig, Gilles Gasparoni, Yongping Li, Eckart Meese, Andreas Keller, and Martin Simon
    Circulating miRNAs are favored for biomarker candidates as they can reflect tissue specific miRNA dysregulation in disease contexts. Moreover, they have additional advantages that they can be monitored in a minimal invasive manner. Blood-borne miRNAs are therefore currently characterized to identify, describe and va...

    Web-based NGS data analysis using miRMaster: a large-scale meta-analysis of human miRNAs
    Tobias Fehlmann, Christina Backes, Mustafa Kahraman, Jan Haas, Nicole Ludwig, Andreas E. Posch, Maximilian L. Würstle, Matthias Hübenthal, Andre Franke, Benjamin Meder, Eckart Meese, Andreas Keller
    The analysis of small RNA NGS data together with the discovery of new small RNAs is among the foremost challenges in life science. For the analysis of raw high-throughput sequencing data we implemented the fast, accurate and comprehensive web-based tool miRMaster. Our toolbox provides a wide range of modules for qua...

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