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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small RNA <span>DNBSEQ™</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. </span><span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ™ Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
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<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of your small RNA transcriptome</li>
<li><strong>Optimal performance on clinical samples</strong>: validated with circulating RNAs from liquid biopsies</li>
<li><strong>Easy to use with minimal hands-on time</strong>: one day, one tube protocol</li>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
<p>Successful CUT&Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 µg of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 µg of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small RNA <span>DNBSEQ™</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. </span><span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ™ Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
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<li><strong>Latest innovation in RNA-seq</strong>: unique D-Plex technology offering ligation-free protocol for library preparation</li>
<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of your small RNA transcriptome</li>
<li><strong>Optimal performance on clinical samples</strong>: validated with circulating RNAs from liquid biopsies</li>
<li><strong>Easy to use with minimal hands-on time</strong>: one day, one tube protocol</li>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
<p>Successful CUT&Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 µg of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 µg of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small RNA <span>DNBSEQ™</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. </span><span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ™ Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
</div>
</div>
</div>
</div>
</div>
</div>
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<li><strong>Latest innovation in RNA-seq</strong>: unique D-Plex technology offering ligation-free protocol for library preparation</li>
<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of your small RNA transcriptome</li>
<li><strong>Optimal performance on clinical samples</strong>: validated with circulating RNAs from liquid biopsies</li>
<li><strong>Easy to use with minimal hands-on time</strong>: one day, one tube protocol</li>
<li><strong>Highest sequencing quality</strong>: specifically formatted for MGI DNBSEQ™ sequencers</li>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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'info1' => '<h2 style="text-align: center;">MicroChIP DiaPure columns after ChIP</h2>
<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
<p>Successful CUT&Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 µg of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 µg of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">D-Plex DNBSEQ Barcodes - Set A</a></li>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
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<p><span>D-Plex Small RNA <span>DNBSEQ™</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time. </span><span>The library preparation takes place in a </span><span>single tube</span><span>, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ™ Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
</div>
</div>
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<li><strong>Latest innovation in RNA-seq</strong>: unique D-Plex technology offering ligation-free protocol for library preparation</li>
<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of your small RNA transcriptome</li>
<li><strong>Optimal performance on clinical samples</strong>: validated with circulating RNAs from liquid biopsies</li>
<li><strong>Easy to use with minimal hands-on time</strong>: one day, one tube protocol</li>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
<p>Successful CUT&Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 µg of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 µg of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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×
