D-Plex mRNA-seq Kit for Illumina

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Catalog Number
24 rxns

Product Features

  • An innovative technology with template switching and UMIs
  • Ultra-sensitive methodology with low input capability down to 10 ng
  • High efficiency for greater gene detection and biomarker identification
  • Consistent gene expression profiling, optimal for gene signature definition
  • Precise measurement of strand orientation for anti-sense transcription detection

Download the manual

The D-Plex mRNA-seq Library Preparation Kit is a tool designed for gene expression analysis. The kit utilizes the D-Plex technology to generate directional libraries for Illumina sequencing directly from total RNAs.

The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from low input samples, down to 10 ng of total RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for gene expression analysis and discovery applications in the coding transcriptome.

The D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been developped to capture  an increased number of genes for great discovery efficiency.

The D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:

Greater gene discovery efficiency at low inputs

total RNA kit

D-Plex mRNA-seq kit enables the detection of higher numbers of genes even for low RNA inputs. Increased number of genes detected at 10x coverage is an indicator of greater sensitivity.

High mappability to the coding transcriptome

total RNA diversity

D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.

Consistent, sensitive gene expression profiling

total RNA

Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.

Exceptional data concordance at low inputs

total RNA

D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.


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