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D-Plex mRNA-seq Kit for Illumina

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Catalog Number
24 rxns

Download the manual

D-Plex mRNA-seq Library Preparation Kit is a tool designed for gene expression analysis. The kit is using the D-Plex technology to generate directionnal libraries for Illumina sequencing directly from total RNAs.

The D-Plex technology utilizes two innovative ligation-free mechanisms - poly(A) tailing and template switching - to produce sequencing libraries from low input samples, down to 10 ng of total RNAs. Combined with unique molecular identifiers (UMI), this complete solution delivers a high-sensitivity detection method for gene expression analysis and discovery applications in the coding transcriptome.

D-Plex mRNA-seq Kit offers a time-saving protocol that can be completed within 6 hours and requires minimal hands-on time. The library preparation takes place in a single tube, increasing the efficiency tremendously. This new solution has been developped to capture  an increased number of genes for great discovery efficiency.

D-Plex mRNA-seq Kit includes all buffers and enzymes necessary for mRNA capture and library preparation. Specific D-Plex Unique Dual Indexes were designed and validated to fit the D-Plex technology and are available separately:

Greater mRNA variant discovery efficiency at low inputs

total RNA kit

D-Plex mRNA-seq kit enables great transcript detection even for low RNA inputs. Increased number of transcripts detected at 10x coverage is an indicator of greater sensitivity.

High mappability to the coding transcriptome

total RNA diversity

D-Plex mRNA-seq libraries capture efficiently coding exons and introns present in the sample of interest.

Consistent, sensitive gene expression profiling

total RNA

Gene expression profiling obtained with D-Plex mRNA-seq kit is conserved across a broad dynamic range of inputs, especially for the lowest RNA inputs.

Exceptional data concordance at low inputs

total RNA

D-Plex mRNA-seq kit produces quality data with high data concordance between low and high RNA inputs. This result demonstrates that D-Plex is an ideal solution for precious samples with limited starting material.

  • Product features
    • Enjoy an innovative technology with template switching and UMI
    • Get high sensitivity data even on picogram inputs and challenging samples
    • Capture an increased number of genes for great discovery efficiency
    • Obatin precise measurement of strand orientation for anti-sense transcription detection
    • Enjoy a simple, easy, single day protocol
  • Indexes

    Specific D-Plex indexes were designed and validated to fit the D-Plex technology for Illumina sequencing and are not included in the kit. They can be bought separately according to your needs. Please choose the format that suits you best among the compatible references to:

    The use of UDI is highly recommended to mitigate errors introduced by read misassignment, including index hopping frequently observed with patterned flow cells such as Illumina’s NovaSeq system.

  • Data analysis

    A specific bioinformatics pipeline has been developed to process the special sequences present in the D-Plex construct, namely the UMI, the A-tail, and the template switch motif. All guidelines and free softwares are shared in the user manual. Subject to the compatibility of D-Plex constructs, other specific pipelines can be used.

    small RNA sequencing bioinformatics pipeline

  •  Documents
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: D-Plex mRNA-seq Kit for Illumina (Diagenode Cat# C05030033). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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