Diagenode

ChIP cross-link Gold

The first step of a ChIP assay is the cross-linking in order to fix the cells. Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. Formaldehyde rapidly permeates the cell membranes and enables a fast cross-linking of closely associated proteins in intact cells. However, formaldehyde is usually not effective to cross-link proteins that are not directly bound to the DNA. For example, chromatin interactions with inducible transcription factors or with cofactors that interact with DNA through protein-protein interactions are not well preserve with formaldehyde. So, for higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChIP cross-link Gold. This reagent is to use in combination with formaldehyde. The protocol involves a sequential fixation. A first protein-protein fixation by the ChIP cross-link Gold followed by protein-DNA fixation by formaldehyde.

Format: supplied as a 250 x concentrated solution
Shipping conditions: shipped at -20°C
Storage conditions: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.

Related products

  • default alt
    C01019027
    ChIP Cross-link Gold

Events

  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics