- After Immunoprecipitation, the magnetic beads binding the antibody/chromatin complex are washed and can then be incubated with the following mix for 1 reaction :
|Tagmentation Buffer (1x)||29 µl|
|Tagmentase loaded||1 µl|
* The quantity of chromatin per reaction will depend on the ChIPmentation experimental design. Successful tagmentation with the proposed protocol has been performed on chromatin from 5,000 to 4,000,000 cells per reaction.
- The reaction is then incubated 10 minutes at 37°C.
- The tagmentation reaction can then be stopped on ice with addition of a buffer containing SDS (0.1%).