Tagmentation Buffer (1x)

Catalog Number
1 ml

Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions. It can be used in combination with Diagenode Tagmentase (Tn5 transposase) on low volume samples (for example DNA or chromatin immobilized on magnetic beads), because it does not require any dilution. This is the reagent used in Diagenode ChIPmentation solutions.

  • Examples of use

    ChIPmentation experiments:

    • After Immunoprecipitation, the magnetic beads binding the antibody/chromatin complex are washed and can then be incubated with the following mix for 1 reaction :
    Tagmentation Buffer (1x) 29 µl
    Tagmentase loaded 1 µl

    *  The quantity of chromatin per reaction will depend on the ChIPmentation experimental design. Successful tagmentation with the proposed protocol has been performed on chromatin from 5,000 to 4,000,000 cells per reaction.

    • The reaction is then incubated 10 minutes at 37°C.
    • The tagmentation reaction can then be stopped on ice with addition of a buffer containing SDS (0.1%).
  •  Documents
  •  Safety sheets
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Tagmentation Buffer (1x) (Diagenode Cat# C01019042-1). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo
    Bestas B. et al.
    Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strategies for mapping off-target effects have emerged, but they suffer from limited sensitivity. To i...


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